Intervention Effect and Mechanism of Qingmei Compound on Acute Gouty Arthritis Based on NLRP3 Pathway

Jingbo WANG; Micun ZHONG; Kun YANG; Panpan ZHU; Xueying TAO; Xiaohui SU; Yueping CHEN; Xiangying KONG

doi:10.13422/j.cnki.syfjx.20221842

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Intervention Effect and Mechanism of Qingmei Compound on Acute Gouty Arthritis Based on NLRP3 Pathway

更新时间：2022-08-11

- Intervention Effect and Mechanism of Qingmei Compound on Acute Gouty Arthritis Based on NLRP3 Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 18, Pages: 70-76(2022)
- 作者机构：
  
  1.广西中医药大学，南宁 530001
  2.中国中医科学院中药研究所，北京 100700
  3.烟台新时代健康产业有限公司，山东烟台 264006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20221842
  CLC： R2-0;R33;R289;R684.3
- Published：20 September 2022，
  
  Published Online：18 July 2022，
  
  Received：12 May 2022，
- 稿件说明：
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王敬博,仲米存,杨坤等.基于NLRP3通路研究青梅复方对急性痛风性关节炎的干预作用及机制[J].中国实验方剂学杂志,2022,28(18):70-76.

WANG Jingbo,ZHONG Micun,YANG Kun,et al.Intervention Effect and Mechanism of Qingmei Compound on Acute Gouty Arthritis Based on NLRP3 Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(18):70-76.
王敬博,仲米存,杨坤等.基于NLRP3通路研究青梅复方对急性痛风性关节炎的干预作用及机制[J].中国实验方剂学杂志,2022,28(18):70-76. DOI： 10.13422/j.cnki.syfjx.20221842.

WANG Jingbo,ZHONG Micun,YANG Kun,et al.Intervention Effect and Mechanism of Qingmei Compound on Acute Gouty Arthritis Based on NLRP3 Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(18):70-76. DOI： 10.13422/j.cnki.syfjx.20221842.

摘要

目的

明确青梅复方对急性痛风性关节炎（AGA）大鼠的治疗作用，初步阐明其作用机制。

方法

将40只雄性SD大鼠按体质量随机分为空白组、模型组、秋水仙碱组（0.3 mg·kg

-1

）、青梅复方低、高剂量组（200、400 mg·kg

-1

），每组8只。模型组及给药组踝关节注射50 g·L

-1

尿酸钠盐（MSU）溶液制备AGA大鼠模型，分别于造模前及造模后6、24、48 h测量大鼠踝关节肿胀指数，采用苏木素-伊红（HE）染色观察AGA大鼠关节组织病理变化，免疫组化检测大鼠关节组织中肿瘤坏死因子-

（TNF-

）、白细胞介素-1

（IL-1

）的表达，蛋白免疫印迹法（Western blot）检测大鼠关节组织中NOD样受体蛋白3（NLRP3）通路及关键蛋白表达。

结果

与空白组比较，模型组大鼠踝关节肿胀指数、滑膜增生及炎性浸润程度显著升高，且踝关节IL-1

、TNF-

、NLRP3蛋白表达及胱天蛋白酶-1（Caspase-1）的剪切体和前体蛋白的比值（Caspase-1 p20/Caspase-1）显著上调（

0.01）。与模型组比较，青梅复方能够明显降低AGA大鼠踝关节肿胀指数，尤其以青梅复方低剂量效果显著（

0.01）；同时青梅复方不仅抑制滑膜增生及炎性浸润（

0.01），且显著下调关节组织中IL-1

、TNF-

、NLRP3蛋白水平及Caspase-1 p20/Caspase-1（

0.01）。

结论

青梅复方能够显著缓解AGA的关节肿胀及炎症浸润，其机制可能与抑制NLRP3信号通路有关。

Abstract

Objective

To investigate the therapeutic effect of Qingmei compound on acute gouty arthritis （AGA） in rats and preliminarily clarify its mechanism.

Method

Forty male SD rats were randomly divided into a blank group， a model group， a colchicine group （0.3 mg·kg

-1

）， and low- and high-dose Qingmei compound groups （200 and 400 mg·kg

-1

）， with eight rats in each group. The AGA model was induced by injecting 50 g·L

-1

monosodium urate （MSU） into the ankle joint of the rats except those in the blank group. The ankle swelling index was measured before and 6， 24， and 48 h after modeling. The pathological changes in the joint tissues of AGA rats were observed by hematoxylin-eosin （HE） staining. The expression of tumor necrosis factor-

（TNF-

） and interleukin-1

（IL-1

） in the joint tissues of rats was detected by immunohistochemistry. The protein expression of NOD-like receptor protein 3 （NLRP3） pathway and key proteins in the joint tissues of rats was detected by Western blot.

Result

Compared with the blank group， the model group showed increased ankle swelling index， synovial hyperplasia， and inflammatory infiltration， and up-regulated expression of IL-1

， TNF-

， and NLRP3 proteins in the ankle joint and the ratio of Caspase-1 shear body to Caspase-1 precursor protein （Caspase-1 p20/Caspase-1）（

0.01）. Compared with the model group， the Qingmei compound groups showed reduced ankle swelling index of AGA rats， especially the low-dose Qingmei compound group （

0.01）. Meanwhile， Qingmei compound inhibited synovial hyperplasia and inflammatory infiltration （

0.01） and reduced the levels of IL-1

， TNF-

， and NLRP3 proteins and Caspase-1 p20/Caspase-1 in joint tissues （

0.01）.

Conclusion

Qingmei Compound can significantly alleviate the joint swelling and inflammatory infiltration of AGA， and its mechanism may be related to the inhibition of the NLRP3 signaling pathway.

关键词

青梅复方急性痛风性关节炎NOD样受体蛋白3（NLRP3）通路大鼠炎症因子

Keywords

Qingmei compoundacute gouty arthritisNOD-like receptor protein 3 （NLRP3） pathwayratinflammatory factor

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