Analysis on Difference of Medicinal Properties Between <italic style="font-style: italic">Atractylodes lancea</italic> and <italic style="font-style: italic">A. chinensis</italic> Based on Correlation of Energy Metabolism and Dryness

Xin ZHAN; Chunlian LIU; Yan WANG; Chengcheng SONG; Xiuli GUO; Shuiqing LI; Yanju LIU

doi:10.13422/j.cnki.syfjx.20220253

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Analysis on Difference of Medicinal Properties Between Atractylodes lancea and A. chinensis Based on Correlation of Energy Metabolism and Dryness

更新时间：2022-08-30

- Analysis on Difference of Medicinal Properties Between Atractylodes lancea and A. chinensis Based on Correlation of Energy Metabolism and Dryness
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 19, Pages: 139-147(2022)
- 作者机构：
  
  湖北中医药大学药学院，湖北省中药炮制工程技术研究中心，武汉 430065
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20220253
  CLC： R22;R28;R96;O657
- Published：05 October 2022，
  
  Published Online：27 January 2022，
  
  Received：11 October 2021，
- 稿件说明：
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詹鑫,刘春莲,王燕等.基于能量代谢与燥性相关性考察茅苍术、北苍术药性差异[J].中国实验方剂学杂志,2022,28(19):139-147.

ZHAN Xin,LIU Chunlian,WANG Yan,et al.Analysis on Difference of Medicinal Properties Between Atractylodes lancea and A. chinensis Based on Correlation of Energy Metabolism and Dryness[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(19):139-147.
詹鑫,刘春莲,王燕等.基于能量代谢与燥性相关性考察茅苍术、北苍术药性差异[J].中国实验方剂学杂志,2022,28(19):139-147. DOI： 10.13422/j.cnki.syfjx.20220253.

ZHAN Xin,LIU Chunlian,WANG Yan,et al.Analysis on Difference of Medicinal Properties Between Atractylodes lancea and A. chinensis Based on Correlation of Energy Metabolism and Dryness[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(19):139-147. DOI： 10.13422/j.cnki.syfjx.20220253.

摘要

目的

探讨苍术燥性与能量代谢的相关性，分析茅苍术、北苍术的药性差异。

方法

将110只健康SD大鼠随机分为11组，分别为正常组、茅苍术挥发油1~5组（S1~S5组，剂量分别为447、473、442、489、496 mg·kg

-1

）和北苍术挥发油1~5组（N1~N5组，剂量分别为197、118、281、222、185 mg·kg

-1

），给药体积均为10 mL·kg

-1

，灌胃给药21 d。通过比较大鼠体质量、饮水量、尿量、全血黏度及颌下腺病理组织切片评估茅苍术、北苍术对大鼠燥性效应的影响，采用免疫组织化学法测定大鼠肾脏中水通道蛋白2（AQP2）的表达，实时荧光定量聚合酶链式反应（Real-time PCR）检测肝脏中细胞色素C氧化酶亚基7A2（COX7A2）、琥珀酸脱氢酶复合体D亚基（SDHD）mRNA的表达量，比色法测定大鼠血浆中琥珀酸脱氢酶（SDH）、乳酸脱氢酶（LDH）和钠离子-钾离子-三磷酸腺苷酶（Na

-K

-ATPase）的含量。应用变异系数法对茅苍术、北苍术质量进行评价，并运用Pearson相关系数分析苍术燥性与能量代谢之间的相关性。

结果

与正常组比较，茅苍术挥发油组与北苍术挥发油组大鼠饮水量、尿量增加，颌下腺腺泡萎缩；茅苍术挥发油组大鼠全血黏度显著增加（

0.01），COX7A2、SDHD mRNA表达水平和SDH、LDH、Na

-K

-ATPase活性均显著上升（

0.01），肾脏AQP2表达量显著降低（

0.01）；北苍术挥发油组COX7A2 mRNA表达水平、SDH活性、全血黏度明显升高（

0.05，

0.01），肾脏AQP2表达量、SDHD mRNA表达水平、LDH和Na

-K

-ATPase活性的差异则均无统计学意义。经各指标综合评分分析，发现茅苍术挥发油对大鼠产生的燥性影响和能量代谢强于北苍术挥发油，燥性指标和能量代谢指标呈正相关。

结论

两类指标均表明茅苍术药性作用强于北苍术，且能量代谢与苍术燥性密切相关。提示从能量代谢角度评价苍术燥性效应具有一定合理性，可以进一步丰富苍术药性评价指标。

Abstract

Objective

To explore the correlation between dryness and energy metabolism of Atractylodis Rhizoma， and to analyze the difference of medicinal properties between

Atractylodes lancea

and

A. chinensis

Method

A total of 110 healthy SD rats were randomly divided into 11 groups， including normal group， volatile oil of

A. lancea

1-5 group （S1-S5 group， doses of 447， 473， 442， 489， 496 mg·kg

-1

） and volatile oil of

A. chinensis

1-5 group （N1-N5 group， doses of 197， 118， 281， 222， 185 mg·kg

-1

）， the administration volume was 0.01 mL·g

-1

with intragastric administration for 21 days. Dryness effect of

A. lancea

and

A. chinensis

on rats was evaluated by comparing the body weight， drinking water volume， urine volume， whole blood viscosity and pathological sections of submandibular gland stained with hematoxylin-eosin （HE）. The expression of aquaporin 2 （AQP2） in rat kidney was measured by immunohistochemistry， the mRNA expressions of cytochrome C oxidase subunit 7A2 （COX7A2） and succinate dehydrogenase （SDH） complex subunit D （SDHD） in liver tissue were detected by real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）. The contents of SDH， lactate dehydrogenase （LDH） and sodium ion-potassium ion-adenosine triphosphatase （Na

-K

-ATPase） in rat plasma were determined by colorimetry. The quality of

A. lancea

and

A. chinensis

was evaluated by coefficient of variation method， and Pearson correlation coefficient was used to analyze the correlation between dryness and energy metabolism.

Result

Compared with the normal group， the amounts of drinking water and urine in volatile oil of

A. lancea

group and volatile oil of

A. chinensis

group increased， and the submandibular gland acini atrophied， the whole blood viscosity of rats in the volatile oil of

A. lancea

group increased significantly （

0.01）， the expression levels of COX7A2 and SDHD mRNA， the activities of SDH， LDH and Na

-K

-ATPase increased significantly （

0.01）， and the expression of AQP2 in kidney decreased significantly （

0.01）. Compared with the normal group， the expression level of COX7A2 mRNA， SDH activity and whole blood viscosity in the volatile oil of

A. chinensis

group increased （

0.05，

0.01）， the AQP2 and SDH mRNA expression levels， LDH and Na

-K

-ATPase activities had no significant difference. The comprehensive score analysis of each index showed that the effect of volatile oil of

A. lancea

on dryness and energy metabolism was stronger than that of volatile oil of

A. chinensis

， and there was a positive correlation between dryness index and energy metabolism index.

Conclusion

The two indexes show that medicinal properties of

A. lancea

is stronger than that of

A. chinensis

， and energy metabolism is closely related to the dryness of Atractylodis Rhizoma. It is suggested that it is reasonable to evaluate the dryness effect of Atractylodis Rhizoma from the perspective of energy metabolism， which can further enrich the evaluation indexes of medicinal properties.

关键词

苍术燥性效应能量代谢中药药性变异系数法Pearson相关性分析挥发油

Keywords

Atractylodis Rhizomadryness effectenergy metabolismChinese materia medica propertycoefficient of variation methodPearson correlation analysisvolatile oil

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⁰

Analysis on Difference of Medicinal Properties Between Atractylodes lancea and A. chinensis Based on Correlation of Energy Metabolism and Dryness

DOI：10.13422/j.cnki.syfjx.20220253

摘要

Abstract

关键词

Keywords

references