五味子木脂素改善衰老小鼠学习记忆能力的机制

郭笑; 叶玉洁; 宋昆; 安丽萍; 盛瑜

doi:10.13422/j.cnki.syfjx.20201226

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五味子木脂素改善衰老小鼠学习记忆能力的机制

药理 | 更新时间：2020-09-09

- 五味子木脂素改善衰老小鼠学习记忆能力的机制
- Mechanism of Schisandrae Chinensis Fructus Lignans in Alleviating Learning and Memory Ability in D-galactose Aging Mice
- 中国实验方剂学杂志 2020年26卷第14期页码：85-91
- 作者机构：
  
  北华大学药学院，吉林吉林 132013
- 作者简介：
  
  郭笑，博士，讲师，从事分子生物学研究，E-mail：395798544@qq.com
  盛瑜，博士，讲师，从事药物分析研究，Tel：0432-64608008，E-mail：383165810@qq.com
- 基金信息：
  
  国家自然科学基金项目(31900918);吉林省教育厅“十三五”科学技术项目(JJKH20180374KJ);吉林省科技发展计划项目(20170311039YY;20180520051JH);吉林省卫生健康青年科技骨干培养计划项目(2019Q020)
- DOI：10.13422/j.cnki.syfjx.20201226
  中图分类号： R22;R242;R2-031;R285.5
- 纸质出版日期：2020-07-20，
  
  网络出版日期：2020-04-15，
- 稿件说明：
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郭笑,叶玉洁,宋昆等.五味子木脂素改善衰老小鼠学习记忆能力的机制[J].中国实验方剂学杂志,2020,26(14):85-91.

GUO Xiao,YE Yu-jie,SONG Kun,et al.Mechanism of Schisandrae Chinensis Fructus Lignans in Alleviating Learning and Memory Ability in D-galactose Aging Mice[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):85-91.
郭笑,叶玉洁,宋昆等.五味子木脂素改善衰老小鼠学习记忆能力的机制[J].中国实验方剂学杂志,2020,26(14):85-91. DOI： 10.13422/j.cnki.syfjx.20201226.

GUO Xiao,YE Yu-jie,SONG Kun,et al.Mechanism of Schisandrae Chinensis Fructus Lignans in Alleviating Learning and Memory Ability in D-galactose Aging Mice[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):85-91. DOI： 10.13422/j.cnki.syfjx.20201226.

摘要

目的

研究五味子木脂素（Schisandrae Chinensis Fructus lignans，SCL）对

-半乳糖（

-galactose，

-gal）所致衰老模型小鼠学习记忆能力的改善作用。

方法

ICR小鼠随机分为4组，即正常组（灌胃蒸馏水，皮下注射生理盐水），模型组（灌胃蒸馏水，皮下注射200 mg·kg

-1

·d

-1

-gal），吡拉西坦组（灌胃吡拉西坦200 mg·kg

-1

·d

-1

，皮下注射

-gal 200 mg·kg

-1

·d

-1

），SCL低剂量组（灌胃SCL 50 mg·kg

-1

·d

-1

，皮下注射

-gal 200 mg·kg

-1

·d

-1

），SCL中剂量组（灌胃SCL 100 mg·kg

-1

·d

-1

，皮下注射

-gal 200 mg·kg

-1

·d

-1

），SCL高剂量组（灌胃SCL 200 mg·kg

-1

·d

-1

，皮下注射

-gal 200 mg·kg

-1

·d

-1

），连续给药10周；通过避暗实验及Morris水迷宫实验观察SCL对

-gal致衰老模型小鼠学习记忆能力的影响；通过化学比色法检测小鼠脑组织中超氧化物歧化酶（superoxide dismutase，SOD）活力及丙二醛（malondialdehyde，MDA）含量；通过聚合酶链式反应（polymerase chain reaction，PCR）检测小鼠脑组织中过氧化物还原酶-6（peroxiredoxin-6，Prdx6），谷胱甘肽过氧化酶1（glutathione peroxidase 1，GSH-Px1）mRNA表达情况；蛋白免疫印迹法（Western blot）检测小鼠组织中Prdx6，GSH-Px1蛋白表达情况。

结果

行为学实验中，与正常组比较，模型组小鼠避暗错误次数明显增加（

0.05），潜伏期显著减少（

0.01），小鼠穿台次数和目标象限停留时间显著减少（

0.01），可作为建模成功的指标。与模型组比较，吡拉西坦组，SCL中、高剂量组小鼠避暗错误次数明显减少（

0.05，

0.01），潜伏期明显延长（

0.05，

0.01），同时，SCL高剂量组小鼠水迷宫穿台次数和目标象限停留时间显著增加（

0.01）。与正常组比较，模型组小鼠脑组织中的SOD活力显著降低（

0.01），MDA含量增加（

0.05），Prdx6，GSH-Px1 mRNA及蛋白表达量明显下降（

0.05，

0.01）。与模型组比较，吡拉西坦组，SCL低、中、高剂量组小鼠脑组织中SOD活力增高（

0.05），MDA水平降低（

0.05），Prdx6，GSH-Px1 mRNA及蛋白的表达水平显著升高（

0.05，

0.01），说明SCL给药组具有剂量依赖性。

结论

SCL能改善

-gal致衰老模型小鼠学习记忆能力，该作用可能与SCL提高小鼠抗氧化能力及上调小鼠脑组织中Prdx6，GSH-Px1的表达水平有关。

Abstract

Objective

To study the effect of Schisandrae Chinensis Fructus lignans (SCL) on learning and memory ability of

-galactose（

-gal）-induced aging model mice.

Method

ICR mice were randomly divided into 4 groups: normal group (distilled water

subcutaneous injection with normal saline)

model group (distilled water

subcutaneous injection with 200 mg·kg

-1

-gal)

piracetam group (oral administration with 200 mg·kg

-1

piracetam

subcutaneous injection with 200 mg·kg

-1

-gal)

low-dose SCL group (oral administration with 50 mg·kg

-1

·d

-1

SCL

subcutaneous injection with 200 mg·kg

-1

·d

-1

-gal)

medium-dose SCL group (oral administration with 100 mg·kg

-1

·d

-1

SCL

subcutaneous injection with 200 mg·kg

-1

·d

-1

-gal)

high-dose SCL group (oral administration with 200 mg·kg

-1

·d

-1

SCL

subcutaneous injection with 200 mg·kg

-1

·d

-1

-gal). The drugs were administered continuously for 10 weeks. Dark test and Morris water maze test were performed to observe the effect of SCL on the learning and memory ability of

-gal-induced aging mice. The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) in mouse brain tissue were detected by chemical colorimetry. The expressions of peroxiredoxin-6（Prdx6） and glutathione peroxidase 1（GSH-Px1） mRNA in mouse brain tissue were detected by polymerase chain reaction (PCR). The expressions of Prdx6 and GSH-Px1 protein in mouse tissues were detected by Western blot.

Result

In behavioral experiments

compared with normal group

the number of dark avoidance errors in model group significantly increased (

0.05)

the latency was significantly reduced (

0.01)

and the number of mouse passes and the target quadrant residence time were significantly reduced (

0.01)

which can be used as an indicator of successful modeling. Compared with the model group

the number of errors in the piracetam group

and medium and high-dose SCL groups was significantly reduced (

0.05，

0.01)

and the latency was significantly prolonged (

0.05，

0.01). At the same time

the number of water maze passes and the target quadrant retention time in the high-dose SCL group increased significantly (

0.01). The results of biochemical indicators showed that compared with normal group

the SOD activity in brain tissue of model group mice was significantly reduced (

0.01)

while the MDA content was increased (

0.05). Compared with the model group

SOD activity in the brain tissues of piracetam group

and low

medium and high-dose piracetam groups was significantly increased (

0.05)

whereas the level of MDA was reduced (

0.05). The expressions of Prdx6 and GSH-Px1 were significantly increased (

0.05，

0.01)

indicating that the SCL administration group was dose-dependent.

Conclusion

SCL can improve the learning and memory ability of

-gal-induced aging mice

which may be related to the anti-oxidation ability of SCL and the up-regulation of Prdx6 and GSH-Px1 expressions in mouse brain tissue.

关键词

五味子木脂素学习记忆能力谷胱甘肽过氧化酶1过氧化物还原酶-6

Keywords

Schisandrae Chinensis Fructus lignanslearning and memory abilityglutathione peroxidase 1（GSH-Px1）peroxiredoxin-6（Prdx6）

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