痰热清注射液与抗生素对多重耐药铜绿假单胞菌外排泵的作用

李悦怡; 李贤煜; 杨伟峰; 王毅; 何忠梅

doi:10.13422/j.cnki.syfjx.20201406

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痰热清注射液与抗生素对多重耐药铜绿假单胞菌外排泵的作用

药理 | 更新时间：2020-09-09

- 痰热清注射液与抗生素对多重耐药铜绿假单胞菌外排泵的作用
- Effect of Tanreqing Injection and Antibiotics on Multi-drug Resistant Pseudomonas aeruginosa Efflux Pump
- 中国实验方剂学杂志 2020年26卷第14期页码：92-98
- 作者机构：
  
  1.吉林农业大学，长春 130000
  2.中国中医科学院，北京 100700
- 作者简介：
  
  李悦怡，在读硕士，从事中药抗菌及中药药理研究工作，E-mail：1668710281@qq.com
  王毅，研究员，博士生导师，从事中西医微生物免疫学研究工作，Tel：010-64019508，E-mail：wangyi02@tsinghua.org.cn
  何忠梅，教授，硕士研究生导师，从事中药有效成分、作用机制研究及产品开发工作，E-mail：heather78@126.com
- 基金信息：
  
  财政部基本科研业务项目(Y201701)
- DOI：10.13422/j.cnki.syfjx.20201406
  中图分类号： R2-0;R22;R285.5;R289
- 纸质出版日期：2020-07-20，
  
  网络出版日期：2020-05-07，
- 稿件说明：
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李悦怡,李贤煜,杨伟峰等.痰热清注射液与抗生素对多重耐药铜绿假单胞菌外排泵的作用[J].中国实验方剂学杂志,2020,26(14):92-98.

LI Yue-yi,LI Xian-yu,YANG Wei-feng,et al.Effect of Tanreqing Injection and Antibiotics on Multi-drug Resistant Pseudomonas aeruginosa Efflux Pump[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):92-98.
李悦怡,李贤煜,杨伟峰等.痰热清注射液与抗生素对多重耐药铜绿假单胞菌外排泵的作用[J].中国实验方剂学杂志,2020,26(14):92-98. DOI： 10.13422/j.cnki.syfjx.20201406.

LI Yue-yi,LI Xian-yu,YANG Wei-feng,et al.Effect of Tanreqing Injection and Antibiotics on Multi-drug Resistant Pseudomonas aeruginosa Efflux Pump[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):92-98. DOI： 10.13422/j.cnki.syfjx.20201406.

摘要

目的

研究痰热清与临床常用抗生素对多重耐药铜绿假单胞菌（multidrug resistant

Pseudomonas aeruginosa

MDR-PA)的相互作用，以及对细菌外排泵的作用研究。

方法

利用抗生素对细菌进行药敏实验。纸片扩散法(Kirby-Bauer

K-B)结合外排泵抑制剂（50 mg·L

-1

）检测抑菌圈直径以及实时荧光定量聚合酶链式反应（Real-time PCR）检测外排泵基因表达量筛选外排泵阳性菌株。KB法观察痰热清（终质量浓度3 g·L

-1

）与抗生素对抑菌圈直径的变化。菌株与痰热清、抗生素亚抑菌浓度共培养进行Real-time PCR检测。KB法观察痰热清持续作用外排泵阳性菌后对抑菌环直径的影响。

结果

经鉴定筛选得到两株MDR-PA外排泵阳性菌株。痰热清与阿洛西林、氨曲南、美罗培南、头孢他啶、头孢哌酮、舒普深均有协同抗菌作用。在抑制细菌外排泵基因表达水平方面，4种药物按作用效果强弱比较为头孢哌酮>痰热清>头孢他啶>舒普深。痰热清持续作用外排泵阳性菌株后与头孢他啶、头孢哌酮、舒普深联合用药疗效较好。

结论

痰热清与头孢他啶，头孢哌酮，舒普深可通过下调细菌外排泵基因表达，使细菌耐药性减弱，降低抗生素临床使用剂量，从而起到抑菌作用。

Abstract

Objective

To study the interaction between Tanreqing injection and commonly used antibiotics against multi-drug resistant

Pseudomonas aeruginosa

(MDR-PA) and the effect on bacterial efflux pump.

Method

Antibiotic susceptibility test was performed with bacteria. Paper diffusion method (Kirby-Bauer

KB) combined with efflux pump inhibitor (50 mg·L

-1

) was used to measure the diameter of the inhibition zone

and real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) was used to detect gene expression of efflux pump Positive efflux pump strain. KB method was used to observe the changes of Tanreqing (final concentration 3 g·L

-1

) and antibiotics on the diameter of the zone of inhibition. Strains were co-cultured with Tanreqing and antibiotic sub-inhibitory concentrations for Real-time PCR detection. KB method was used to observe the effect of Tanreqing on the diameter of bacteriostatic ring after the continuous use of efflux pump-positive bacteria.

Result

Two MDR-PA efflux pump-positive strains were identified and screened. Tanreqing has synergistic antibacterial effect with aloxicillin

aztreonam

meropenem

ceftazidime

cefoperazone

and Shupushen. In inhibiting the expression levels of bacterial efflux pump genes

the four drugs were compared by the effect: cefoperazone>Tanreqing>ceftazidime>Shupushen. After Tanreqing continued to act on efflux pump-positive strains

it could have a better effect in combination with ceftazidime

cefoperazone

and Shupushen.

Conclusion

Tanreqing

ceftazidime

cefoperazone

and Shupushen can reduce the drug resistance of bacteria by down-regulating the expressions of bacterial efflux pump genes

and reducing the clinical dose of antibiotics

and thus play a bacteriostatic effect.

关键词

痰热清注射液多重耐药铜绿假单胞菌外排泵

Keywords

Tanreqingmulti-drug resistant Pseudomonas aeruginosaefflux pump

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