饮片巴戟天、巴戟肉和盐巴戟天主要化学成分的含量差异分析

余意; 康利平; 郝庆秀; 周利; 杨健; 朱龙波; 胡明华; 马方励; 郭兰萍

doi:10.13422/j.cnki.syfjx.20202063

您当前的位置：

首页 >

文章列表页 >

饮片巴戟天、巴戟肉和盐巴戟天主要化学成分的含量差异分析

药学基础 | 更新时间：2021-12-03

- 饮片巴戟天、巴戟肉和盐巴戟天主要化学成分的含量差异分析
- Analysis on Contents of Main Constituents in Prepared Morindae Officinalis Radix，Morindae Officinalis Radix Processed with Steaming and Salt
- 中国实验方剂学杂志 2021年27卷第2期页码：146-152
- 作者机构：
  
  1.无限极（中国）有限公司，广州 510663
  2.中国中医科学院中药资源中心道地药材国家重点实验室培育基地，无限极中草药品质研究联合实验室，北京 100700
  3.北京中研百草检测认证有限公司，北京 100700
- 作者简介：
  
  余意，博士，工程师，从事中药材及保健食品研究，Tel：020-80734767，E-mail：Yi.Yu@infinitus-int.com
  郝庆秀，博士，副研究员，从事中药质量研究，Tel：010-64087964，E-mail：haoqingxiu@163.com
  郭兰萍，博士，研究员，从事中药资源生态研究，Tel：010-64087856，E-mail：glp01@126.com
- 基金信息：
  
  国家重点研发计划项目(2020YFC1712700;2017YFC1700701);国家自然科学基金重大项目(81891014)
- DOI：10.13422/j.cnki.syfjx.20202063
  中图分类号： R284.2;R289;R22;R2-031
- 纸质出版日期：2021-01-20，
  
  网络出版日期：2020-07-28，
  
  收稿日期：2019-12-06，
- 稿件说明：
扫描看全文
余意,康利平,郝庆秀等.饮片巴戟天、巴戟肉和盐巴戟天主要化学成分的含量差异分析[J].中国实验方剂学杂志,2021,27(02):146-152.

YU Yi,KANG Li-ping,HAO Qing-xiu,et al.Analysis on Contents of Main Constituents in Prepared Morindae Officinalis Radix，Morindae Officinalis Radix Processedwith Steaming and Salt[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(02):146-152.
余意,康利平,郝庆秀等.饮片巴戟天、巴戟肉和盐巴戟天主要化学成分的含量差异分析[J].中国实验方剂学杂志,2021,27(02):146-152. DOI： 10.13422/j.cnki.syfjx.20202063.

YU Yi,KANG Li-ping,HAO Qing-xiu,et al.Analysis on Contents of Main Constituents in Prepared Morindae Officinalis Radix，Morindae Officinalis Radix Processedwith Steaming and Salt[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(02):146-152. DOI： 10.13422/j.cnki.syfjx.20202063.

摘要

目的

考察目前市场上的饮片巴戟天、巴戟肉和盐巴戟天中主要化学成分的含量差异。

方法

在主要产区和国内主流药材市场上，收集饮片巴戟天41批、巴戟肉32批、盐巴戟天10批；在前期对巴戟天药材中糖类成分研究的基础上，利用超高液相色谱飞行时间质谱（UPLC-Q-TOF-MS）技术鉴别饮片巴戟天、巴戟肉和盐巴戟天中的主要的差异成分，利用UPLC对主要成分含量进行测定，并结合热图对三者的主要化学成分的含量进行差异分析。

结果

饮片巴戟天、巴戟肉和盐巴戟天三者的主要成分有显著差别，表现在果糖、葡萄糖、蔗糖以及低聚糖GF

的含量及种类两方面。巴戟肉和盐巴戟天中10种低聚糖GF

（

=2~11）的含量均分别低于饮片巴戟天中相应成分的含量（

0.01），巴戟肉中果糖、葡萄糖、蔗糖的含量均高于巴戟天和盐巴戟天中相应成分的含量，差异极显著（

0.01）。饮片巴戟天、巴戟肉和盐巴戟天中水晶兰苷的质量分数分别为42.6，39.8，32.3 mg·g

-1

，其中巴戟天与盐巴戟天中的含量差异显著（

=0.031）；各批次的巴戟天和盐巴戟天中耐斯糖的质量分数均高于4.0%，高于2015年版《中国药典》（简称“药典”）标准的要求；而巴戟肉中耐斯糖的平均质量分数为14.7 mg·g

-1

，只有少数批次的巴戟肉符合药典要求。

结论

蒸制使巴戟天中大部分的低聚糖转化成了果糖、葡萄糖、蔗糖；盐制使巴戟天中的各低聚糖和水晶兰苷的含量均显著降低，但是，各低聚糖含量降低的程度远小于巴戟肉。巴戟天和盐巴戟天的耐斯糖含量符合药典质量要求，巴戟肉中耐斯糖含量低于药典中限量要求。建议开展巴戟天药典标准的提升和修订，以保持药典的科学性、先进性和适用性，增强药典对巴戟天药材质量控制的导向作用。该研究结果为饮片巴戟天、巴戟肉、盐巴戟天的质量标准修订及其功效物质基础研究提供了参考依据。

Abstract

Objective

To investigate the differences of main components of prepared Morindae Officinalis Radix，Morindae Officinalis Radix processed with steaming and salt.

Method

A total of 83 batches samples were collected in the market， including 41 batches of prepared Morindae Officinalis Radix，32 batches of Morindae Officinalis Radix processed with steaming and 10 batches of Morindae Officinalis Radix processed with salt. The contents of main components were determined with high performance liquid chromatography coupled with four-pole tandem time-of-flight mass spectrometry（UPLC-Q-TOF-MS），and the differences were analyzed.

Result

The main components of prepared Morindae Officinalis Radix and Morindae Officinalis Radix processed with salt were fructo-oligosaccharides （GF

），monotropein. The main components of Morindae Officinalis Radix processed with steaming were fructose，glucose，sucrose，and monotropein. The main differences of prepared Morindae Officinalis Radix and

Morindae Officinalis Radix processed with steaming and salt were the contents of fructose，glucose，sucrose and GF2-GF11. The contents of GF2-GF11 in Morindae Officinalis Radix processed with steaming and salt were all lower than those in prepared Morindae Officinalis Radix，with extremely significant differences（

0.01）. The contents of fructose，glucose and sucrose in

Morindae Officinalis Radix processed with steaming were significantly higher than those in prepared Morindae Officinalis Radix. The content of GF3 in each batch was higher than 40.0 mg·g

-1

in prepared Morindae Officinalis Radix and Morindae Officinalis Radix processed with salt，and significantly higher than the limit in

Chinese Pharmacopoeia

. However，there were only a few batches of Morindae Officinalis Radix processed with steaming in line with the requirements of

Chinese Pharmacopoeia

. The contents of monotropein in processing Morindae Officinalis Radix and Morindae Officinalis Radix processed with steaming and salt were 42.6，39.8，32.3 mg·g

-1

，respectively. The content of monotropein in prepared Morindae Officinalis Radix was higher than that in Morindae Officinalis Radix processed with steaming. The content of monotropein in Morindae Officinalis Radix processed with steaming was higher than that in Morindae Officinalis Radix

processed with salt. Compared with the components of GF2-GF11，the effect of processing with steaming process and/or salt on monotropein content was relatively less.

Conclusion

The contents of GF2-GF11 components in prepared Morindae Officinalis Radix were converted into fructose，glucose and sucrose after processing with steaming and/or salt. The results showed that the content limit of Morindae Officinalis Radix processed with steaming needs to be revised in line with the requirements of

Chinese Pharmacopoeia

for the quality control of Morindae Officinalis Radix. The results provide a reference basis for revising the quality standards and studying the pharmacodynamic material basis of prepared Morindae Officinalis Radix，Morindae Officinalis Radix processed with steaming and salt.

关键词

巴戟天巴戟肉盐巴戟天成分含量差异分析

Keywords

prepared Morindae Officinalis RadixMorindae Officinalis Radix processed with steamingMorindae Officinalis Radix processed with saltcomponentcontentdifferential analysis

references

AN L，YANG J C，YIN H，et al.Inulin-type oligosaccharides extracted from yacon produce antidepressant-like effects in behavioral models of depression［J］.Phytother Res，2016，30（12）：1937-1942.

饶鸿宇，陈滔彬，何彦，等.南药巴戟天化学成分与药理研究进展［J］.中南药学，2018，16（11）：1567-1574.

黄世敬，张颖，王彦云.巴戟天抗抑郁研究进展［J］.世界中西医结合杂志，2013，8（7）：744-746.

娄勇军，王佳，黄玉秋，等.巴戟天及其炮制品对肾阳虚大鼠HPA 轴功能的改善作用［J］.中成药，2018，40（11）：2535-2539.

翟旭峰，黄玉秋，史辑，等，盐巴戟对肾阳虚大鼠下丘脑-垂体-甲状腺轴的调节作用［J］.中成药，2017，39（2）：233-237.

CHOI J，LEE K T，CHOI M Y，et al.Antinociceptive anti-inflammatory effect of Monotropein isolated from the root of Morinda officinalis［J］.Biol Pharm Bull，2005，28（10）：1915-1918.

ZHANG J H，XIN H L，XU Y M，et al.Morinda officinalis How-A comprehensive review of traditional uses，phytochemistry and pharmacology［J］.J Ethnopharmacol，2018，213： 230-255.

VARKI A.Biological roles of oligosaccharides：all of the theories are correct［J］.Glycobiology，1993，3（2）：97-130.

QIU Z L，LIU C L，GAO Z L，et al.The inulin-type oligosaccharides extract from Morinda officinalis，a traditional Chinese herb，ameliorated behavioral deficits in an animal model of post-traumatic stress disorder［J］.Metabolic Brain Dise，2016，31（5）：1143-1149.

MENSINK M A，FRIJLINK H W，VAN DER V M K，et al.Inulin，a flexible oligosaccharide II：Review of its pharmaceutical applications［J］.Carbohydr Polym，2015，134：418-428.

JOHNSON H K C，PINNELL L J，WASKOW A M，et al.Short-chain fructo-oligosaccharide and inulin modulate inflammatory responses and microbial communities in Caco2-bbe cells and in a mouse model of intestinal injury［J］.J Nutr，2014，144（11）：1725-1733.

郝庆秀，康利平，朱寿东，等.基于UPLC-QTOF/MSE技术的巴戟天低聚糖成分的快速鉴别研究［J］.中国中药杂志，2018，43（6）：1201-1208.

HAO Q X，NAN T G，ZHOU L，et al.Rapid simultaneous quantification of fructooligosaccharides in Morinda officianalis by ultra‐high performance liquid chromatography［J］.J Sep Sci，2019，42（13）：2222-2230.

陈红裴，占柱，程再兴，等.巴戟天与生巴戟天中水晶兰苷含量对比研究［J］.中医学报，2013，28（10）：1504-1505.

刘瑾，徐吉银，罗进辉，等.不同产地巴戟天中水晶兰苷的含量测定［J］.中成药，2010，32（3）：517-519.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

山豆根中生物碱类成分的毒性及机制研究进展

紫外分光光度法测定骨宝昕肾片中总黄酮的含量

高分离度快速液相色谱法测定不同产地枳实中主要黄酮类成分的含量