Neuroprotective Mechanism of Buyang Huanwu Tang on Experimental Autoimmune Encephalomyelitis Mice

Jian-chun LIU; Hong-zhen ZHANG; Wen-juan GUO; Zhi CHAI; Jie-zhong YU; Jing-wen YU; Bao-guo XIAO; Cun-gen MA

doi:10.13422/j.cnki.syfjx.20191340

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Neuroprotective Mechanism of Buyang Huanwu Tang on Experimental Autoimmune Encephalomyelitis Mice

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- Neuroprotective Mechanism of Buyang Huanwu Tang on Experimental Autoimmune Encephalomyelitis Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 14, Pages: 55-61(2019)
- 作者机构：
  
  1.山西中医药大学　神经生物学研究中心，晋中　 030619；
  2.大同大学　脑科学研究所，山西　大同　 037009；
  3.复旦大学　附属华山医院　神经病学研究所，上海　 200025
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191340
  CLC： R289; R246.6; R392; R322.81
- Published：20 July 2019，
  
  Published Online：19 March 2019，
  
  Received：16 November 2018，
- 稿件说明：
扫描看全文
Jian-chun LIU, Hong-zhen ZHANG, Wen-juan GUO, et al. Neuroprotective Mechanism of Buyang Huanwu Tang on Experimental Autoimmune Encephalomyelitis Mice. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):55-61(2019)
DOI：

Jian-chun LIU, Hong-zhen ZHANG, Wen-juan GUO, et al. Neuroprotective Mechanism of Buyang Huanwu Tang on Experimental Autoimmune Encephalomyelitis Mice. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):55-61(2019) DOI： 10.13422/j.cnki.syfjx.20191340.

摘要

目的：

探讨补阳还五汤(BYHWT)在实验性自身免疫性脑脊髓炎(EAE)发展的不同阶段的神经保护作用和机制。

方法：

采用髓鞘少突胶质细胞糖蛋白35-55多肽(MOG

35-55

)诱导36只C57BL/6雌性小鼠建立实验性自身免疫性脑脊髓炎(EAE)模型，随机分为EAE 9，17，28 d组以及BYHWT 9，17，28 d组。BYHWT各组于免疫后第3天开始灌胃给药(50 g·kg

－1

·d

－1

)，EAE组以相同方式等体积给予生理盐水，每天1次，连续至免疫后9，17，28 d。采用国际通用的五级临床症状评分观察BYHWT对EAE小鼠的干预作用；采集小鼠脊髓标本，进行苏木素-伊红(HE)染色和固蓝(LFB)染色观察BYHWT的神经保护作用；采用蛋白免疫印迹法(Western blot)检测脊髓神经营养因子(BDNF)和生长相关蛋白-43(GAP-43)等的表达。

结果：

补阳还五汤治疗后可明显抑制脊髓炎细胞浸润，减轻髓鞘脱失；与EAE组比较，BYHWT各给药组Nogo-A在脊髓表达显著下调(

0.01)，与EAE 17，28 d组比较，BYHWT 17 d组和28 d组BDNF在脊髓中表达明显上调(

0.05，

0.01)；与EAE 9，17 d组比较，BYHWT 9，17 d组GAP-43在脊髓中表达显著上调(

0.01)。

结论：

补阳还五汤可以通过上调NTFs类物质的表达，下调神经抑制因子的表达，改善局部神经生长微环境而发挥神经保护的作用。

Abstract

Objective:

To explore the neuroprotective effect and mechanism of Buyang Huanwu Tang (BYHWT) on experimental autoimmune encephalomyelitis (EAE) at different stages.

Method:

The 36 female C57BL/6 mice were immunized subcutaneously with myelin oligodendrocyte glycoprotein peptides (MOG

35-55

)

then randomly divided into 9

28 d EAE control group. Each BYHWT group was orally given drugs on the 3

day after immunization (50 g·kg

-1

·d

-1

)

and EAE control group was given the same volume of normal saline in the same way once a day for 9

17 and 28 d after immunization. The effect of BYHWT on EAE mice was observed with internationally accepted clinical score. Brain and spinal cord specimens were collected at 9

17 and 28 d after immunization. The neuroprotective effect of BYHWT was observed by hematoxylin-eosin(HE)staining and solid blue staining (LFB). The expressions of BDNF and GAP-43 in spinal cord and brain were detected by Western blot.

Result:

After treatment

BYHWT can significantly inhibit myelitis cell infiltration and alleviate myelin loss. Compared with EAE group

the expression of Nogo-A in the spinal cord of each BYHWT group was significantly down-regulated (

0.01)

and the expression of BDNF in the spinal cord was significantly up-regulated (

0.05

0.01) in the BYHWT group 17 and 28 d group compared with EAE group 17 and 28 d group. Compared with EAE 9

17 d group

GAP-43 expression was significantly up-regulated in the spinal cord of BYHWT 9

17 d group (

0.01).

Conclusion:

BYHWT can improve the local nerve growth microenvironment and promote the expression of NTFs

reduce the expressions of neuroinhibitory factors

and play a role in neuroprotection.

关键词

补阳还五汤多发性硬化脑源性神经营养因子生长相关蛋白-43Nogo-A蛋白神经生长微环境

Keywords

Buyang Huanwu Tangexperimental autoimmune encephalomyelitisbrain-derived neurotrophic factorgrowth-related protein-43Nogo-A proteinnerve growth microenvironment

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