Isolation and Identification of Chemical Constituents from Ethanol Extract of Camptosorus sibiricus

Ling-yue LYU; Dai-zhou ZHANG; Fang WANG; Qing-wen JIA; Da-lian LIANG; Min LI; Wei ZHANG

doi:10.13422/j.cnki.syfjx.20191413

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Isolation and Identification of Chemical Constituents from Ethanol Extract of Camptosorus sibiricus

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- Isolation and Identification of Chemical Constituents from Ethanol Extract of Camptosorus sibiricus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 14, Pages: 168-172(2019)
- 作者机构：
  
  1.山东中医药大学，济南　 250355；
  2.山东省药学科学院，济南　 250101；
  3.青岛市中医院，山东　青岛　 266033
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191413
  CLC： R284.2; R289; R22; R2-031
- Published：20 July 2019，
  
  Published Online：04 April 2019，
  
  Received：30 October 2018，
- 稿件说明：
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Ling-yue LYU, Dai-zhou ZHANG, Fang WANG, et al. Isolation and Identification of Chemical Constituents from Ethanol Extract of Camptosorus sibiricus. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):168-172(2019)
DOI：

Ling-yue LYU, Dai-zhou ZHANG, Fang WANG, et al. Isolation and Identification of Chemical Constituents from Ethanol Extract of Camptosorus sibiricus. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):168-172(2019) DOI： 10.13422/j.cnki.syfjx.20191413.

摘要

目的：

研究过山蕨

Camptosorus sibiricus

全草95%乙醇提取物的化学成分，并测定其抗氧化活性。

方法：

采用正相硅胶，小孔树脂(MCI)，LH-20型羟丙基葡聚糖凝胶(Sephadex LH-20)，制备薄层色谱和反相HPLC等多种色谱技术进行分离纯化，通过波谱数据分析，结合文献鉴定了化合物的结构。

结果：

共分离得到11个化合物，分别鉴定为香豆酸(1)，香豆酸 4-

-吡喃葡萄糖苷(2)，顺式对香豆酸4-

-吡喃葡萄糖苷(3)，阿魏酸4-

-吡喃葡萄糖苷(4)，咖啡酸甲酯(5)，阿魏酸甲酯(6)，丁香酸(7)，丁香酸-4-

-吡喃葡萄糖苷(8)，原儿茶醛(9)，香草醛(10)，丁香醛(11)。

结论：

化合物3～11均为首次从过山蕨属中分离得到。对从过山蕨乙醇提取物中分离的11个化合物采用类超氧化物歧化酶(SOD-like)活性测试法进行了抗氧化活性研究，结果显示化合物7，9～11均有一定活性，四者的半抑制浓度(IC

)分别为16.70，11.70，12.23，13.52 μmol·L

－1

；在浓度1.0×10

－4

mol·L

－1

下，其他化合物均未表现出活性。

Abstract

Objective:

To study the chemical constituents in 95%ethanol extract of the whole plant of

Camptosorus sibiricus

and determine its antioxidant activity.

Method:

Compounds were isolated by a combination of various chromato-graphic techniques

including column chromatography over silica gel and Sephadex LH-20 and reversed-phase HPLC. Their structures were identified on the basis of physicochemical properties and spectral data reported in the literature.

Result:

Eleven compounds were identified as

trans

-coumaric acid (1)

trans

-coumaric acid 4-

-glucoside (2)

cis

-coumaric acid 4-

-glucoside (3)

(

)-ferulic acid-4-

-glucoside (4)

caffeic acid methyl ester (5)

ferulic acid methyl ester (6)

syringic acid (7)

syringic acid-4-

-glucopyranoside (8)

protocatechualdehyde (9)

vanillain (10) and syringaldehyde (11)

respectively.

Conclusion:

Compounds 3-11 are isolated from the genus

Camptosorus

for the first time. In the

in vitro

SOD-like activity assays

compounds 7

9-11 show an antioxidant activity with half maximal inhibitory concentration(IC

)values of 16.70

11.70

12.23 and 13.52 μmol·L

-1

respectively.

关键词

过山蕨95%乙醇提取物化学成分结构鉴定抗氧化活性

Keywords

Camptosorus sibiricus95%ethanol extractchemical constituentsstructure identificationantioxidant activity

references

严仲铠，李万林．中国长白山药用植物彩色图志[M]．北京：人民卫生出版社，1997：96．

李宁．过山蕨和山茶种子的化学成分和药理活性研究[D]．沈阳：沈阳药科大学，2006．

ZHANG P, CHENG Y Y, MA Z J, et al. New cycloartane glycosides from Camptosorus sibiricus Rupr. [J].J Asian Nat Prod Res, 2008, 10(11): 1069-1074.

沈阳药学院药学四十一期队．过山蕨的扩张血管作用和毒性[J]．沈阳药学院学报，1977，7(7)：34-37．

沈阳药学院药学四十一期队．过山蕨治疗脉管炎有效部位的实验研究[J]．沈阳药学院学报，1977，7(38)：38-43．

徐绥绪．过山蕨治疗心血管病的研究．5．过山蕨治疗脉管炎有效成分的研究[J]．沈阳药学院学报，1979，36(11)：36-46．

梁大连，程艳玲，董世波，等．过山蕨总黄酮对大鼠血栓闭塞性脉管炎的改善作用[J]．现代药物与临床，2011，26(6)：481-484．

张宝凤，周荣汉，董淑华，等．过山蕨治疗心血管病的研究[J]．沈阳药学院学报，1979，11(29)：29-35．

梁大连，于亮，李宁，等．过山蕨水提物的化学成分[J]．沈阳药科大学学报，2017，34(7)：541-545，552．

郭娟丽，张培成，张智武．油菜花粉的化学成分研究[J]．中国中药杂志，2009，34(10)：1235-1237．

吴慧星，李晓帆，李荣，等．番石榴叶中抗氧化活性成分的研究[J]．中草药，2010，41(10)：1593-1597．

徐璐，李艳茸，李春，等．藏药甘青乌头化学成分研究[J]．中国中药杂志，2013，38(17)：2818-2825．

马双成，毕培曦，黄荣春，等．金银花药材中抗呼吸道病毒感染的咖啡酰奎宁酸类成分的定量研究[J]．药物分析杂志，2005，25(7)：751-755．

Pieters L, Dyck S V, Gao M, et al. Synthesis and biological evaluation of dihydrobenzofuran lignans and related compounds as potential antitumor agents that inhibit tubulin polymerization [J].J Med Chem, 1999, 42: 5475-5481.

CHANG Y C, CHANG R F, WU Y C. The constituents of Lindera glauea [J].J Chin Chem Soc, 2000, 47: 373-380.

Kashiwada Y, Nonaka G I, Nishioka I. Tannins and related compounds. XLV. Rhubarb.(5): isolation and characterization of flavan-3-ol and procyanidin glucosides [J].Chem Pharm Bull, 1986, 34(8): 3208-3222.

赵爱华，赵勤实，李蓉涛，等．肾茶的化学成分研究[J]．云南植物研究，2004，26(5)：563-568．

羊晓东，梅双喜，杨蓉，等．云南兔耳草的化学化学成分研究[J]．天然产物研究与开发，2002，14(6)：1-3．

Schwartz A. Efficient synthesis of 3, 4, 5-trimethoxybenzaldehyde via reissert aldehyde synthesis [J].J Org Chem, 1982, 47(11): 2213-2214.

LI N, LI X, ZHANG Y, et al. Free radical scavengers, antioxidants and aldose reductase inhibitors from Camptosorus sibiricus Rupr [J].Z Naturforsch C, 2008, 63(1/2): 66-68.

王业玲．石见穿化学成分及其生物活性研究[D]．沈阳：沈阳药科大学，2008．

王新玲，王晓梅，胡君萍，等．新疆鼠尾草不同部位不同极性提取物抗氧化活性[J]．中国实验方剂学杂志，2017，23(23)：62-65．

肖凯，冯大伟，杨金丽，等．盔状黄芩中的苯乙醇苷及黄酮苷类化合物分析[J]．中国实验方剂学杂志，2017，23(20)：36-41．

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