Isolation and Identification of Chemical Constituents from Cinnamomi Ramulus

Ling-li ZHU; Zhi-fu AI; Li XU; Yong-liang JIN; Chun-ling LIU; Feng LIU; Hua LIU

doi:10.13422/j.cnki.syfjx.20190712

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Isolation and Identification of Chemical Constituents from Cinnamomi Ramulus

Resource and Quality Evaluation | 更新时间：2021-02-09

- Isolation and Identification of Chemical Constituents from Cinnamomi Ramulus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 14, Pages: 173-178(2019)
- 作者机构：
  
  1.江西中医药大学　药学院，南昌　 330004；
  2.江西中医药大学　研究生院，南昌　 330004；
  3.樟树市人民医院，江西　樟树　 331200；
  4.江西中医药大学　附属医院，南昌　 330006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190712
  CLC： R284.2; R289; R22; R2-031
- Published：20 July 2019，
  
  Published Online：19 December 2018，
  
  Received：21 October 2018，
- 稿件说明：
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Ling-li ZHU, Zhi-fu AI, Li XU, et al. Isolation and Identification of Chemical Constituents from Cinnamomi Ramulus. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):173-178(2019)
DOI：

Ling-li ZHU, Zhi-fu AI, Li XU, et al. Isolation and Identification of Chemical Constituents from Cinnamomi Ramulus. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):173-178(2019) DOI： 10.13422/j.cnki.syfjx.20190712.

摘要

目的：

明确桂枝的活性组分，对75%乙醇提浸膏进行系统化学成分分离与纯化，以期找到抑制necroptosis的目标小分子化合物。

方法：

取干燥桂枝20 kg，粉碎后用75%乙醇冷浸提取，提取4次，7 d 1次，减压回收溶剂得到总浸膏。将所得总浸膏，运用D101大孔吸附树脂柱色谱进行分离，依次用水，30%乙醇，50%乙醇，70%乙醇，90%乙醇洗脱，减压回收溶剂得水部分，30%乙醇部分，50%乙醇部分，70%乙醇部分，90%乙醇部分。对30%乙醇部分，50%乙醇部分联合采用常压硅胶柱色谱，LH-20型羟丙基葡聚糖凝胶(Sephadex LH-20)柱色谱，高压制备色谱，重结晶等方法进行分离纯化，并根据单体化合物理化性质与波谱学数据(

H-NMR，

C-NMR，MS)等进行结构鉴定。

结果和结论：

从桂枝中分离得到13个化合物，分别鉴定为(＋)-丁香脂素(1)，(＋)-南烛木树脂酚(2)，山橘脂酸(3)，裂异落叶松脂醇(4)，ovafolinin B(5)，原儿茶醛(6)，原儿茶酸(7)，丁香醛(8)，香草酸(9)，原儿茶酸乙酯(10)，丁香酸(11)，没食子酸乙酯(12)，2-(3′，4′-二羟苯基)-1，3-胡椒环-5-醛(13)。其中1～5，10～13为首次从该植物中分离得到。

Abstract

Objective:

The research group found in the early stage that the 75%alcohol extract of the Cinnamomi Ramulus had a significant physiological activity in inhibiting necroptosis by screening out the self-built sample library of 100 kinds of traditional Chinese medicines in Jiangxi. To identify the active components and find the target compounds

the 75%alcohol extracts of Cinnamomi Ramulus were isolated and studied systemically in chemistry.

Method:

The 20 kg dry Cinnamomi Ramulus was crushed into coarse powder

and extracted with 75%alcohol for four times

one time every 7 d. Then total extracts were obtained after solvent was recycled under decompression. The extract was separated by D101 macroporous resin column chromatography and eluted by water

30%ethanol

50%ethanol

70%ethanol

90%ethanol

so as to get the corresponding fraction finally. The compounds in the 30%ethanol and 50%ethanol fraction were isolated and purified by chromatography on silica gel

Sephadex LH-20 column and high pressure preparative chromatography

and their structures were determined according to physicochemical properties and spectral analysis.

Result and Conclusion:

Thirteen compounds were isolated and identified as (+ )-syringaresinol (1)

(+ )-lyoniresinol (2)

spicatolignan B (3)

(-)-secoisolariciresinol (4)

ovafolinin B (5)

protocatechualdehyde (6)

protocatechuic acid (7)

syringaldehyde (8)

vanillic acid (9)

ethyl protocatechuate (10)

syringic acid (11)

ethyl gallate (12)

2-(3′

4′-dihydroxyphenyl)-1

3-pepper ring-5-aldehyde (13). Compounds 1-5

10-13 were isolated from this plant for the first time.

关键词

桂枝75%乙醇部位化学成分结构鉴定

Keywords

Cinnamomi Ramulus75%alcohol extractchemical constituentsstructural identification

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