Standard Decoction of Saposhnikoviae Radix Pieces

Wen-zheng CAO; Wen-long SU; Han-yang LI; Yue-ting YU; Xin-xin SU; He-cheng XIA; Hong-mei GAO

doi:10.13422/j.cnki.syfjx.20190814

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Standard Decoction of Saposhnikoviae Radix Pieces

Resource and Quality Evaluation | 更新时间：2021-02-09

- Standard Decoction of Saposhnikoviae Radix Pieces
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 14, Pages: 192-198(2019)
- 作者机构：
  
  长春中医药大学　药学院，长春　 130117
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190814
  CLC： R284.1; R289; R22; R2-031
- Published：20 July 2019，
  
  Published Online：07 January 2019，
  
  Received：28 July 2018，
- 稿件说明：
扫描看全文
Wen-zheng CAO, Wen-long SU, Han-yang LI, et al. Standard Decoction of Saposhnikoviae Radix Pieces. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):192-198(2019)
DOI：

Wen-zheng CAO, Wen-long SU, Han-yang LI, et al. Standard Decoction of Saposhnikoviae Radix Pieces. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):192-198(2019) DOI： 10.13422/j.cnki.syfjx.20190814.

摘要

目的：

制备防风饮片标准汤剂，并进行质量标准的研究，为其临床应用及其他饮片标准汤剂的研究提供科学参考。

方法：

采用传统水煎煮方法，制备11批防风饮片标准汤剂，进行pH，升麻素苷及5-

-甲基维斯阿米醇苷含量的测定，计算其出膏率与转移率，并对测定方法可行性进行考察。建立HPLC指纹图谱分析方法，采用Hypersil ODS2色谱柱(4.6 mm×250 mm，5 μm)，流动相甲醇-进行梯度洗脱，检测波长254 nm，流速1 mL·min

－1

，柱温25 ℃，并进行相似度评价。

结果：

11批防风饮片标准汤剂的pH均为5.5，出膏率在34.3%～46.3%，平均出膏率为41.4%，标准偏差为3.7%；升麻素苷的转移率在66.8%～93.5%，平均转移率为79.4%，标准偏差为12.1%；5-

-甲基维斯阿米醇苷的转移率在70.4%～98.2%，平均转移率为83.4%，标准偏差为10.8%。各项方法学考察结果RSD均符合要求。指纹图谱中确定了9个主要共有峰，其相似度

0.9。

结论：

该研究建立的质量评价方法稳定性、精密度和重复性良好，指纹图谱的相似度高，适用于防风饮片标准汤剂的质量评价，并且可为其他相关制剂的质量评价提供科学依据。

Abstract

Objective:

To prepare standard decoction of Saposhnikoviae Radix pieces

and conduct a study on the quality standards

in order to provide the scientific reference for the study of clinical application and other decoction standard decoctions.

Method:

By the traditional water decocting method

11 batches of standard decoction of Saposhnikoviae Radix pieces were prepared to determine pH

prim-

-glucosylcimifugin and 4′-

-glucosyl-5-

-methylvisamminol content

calculate the paste rate and transfer rate

and investigate the feasibility of the measurement method. An analytical method for HPLC fingerprint was established. Hypersil ODS2 column (4.6 mm×250 mm

5 μm) was adopted and eluted with methanol-water as the mobile phase in a gradient mode. The detection wavelength was 254 nm

the flow rate was 1 mL·min

-1

and the column temperature was 25 ℃. The fingerprint analysis method was used to evaluate the similarity of the 11 batches of standard decoction of Saposhnikoviae Radix pieces.

Result:

Through the determination of 11 batches of standard decoctions of Saposhnikoviae Radix pieces

the average pH value was 5.5

the paste-out rates were between 34.3% and 46.3%

and the average paste-out rate was 41.4%

the standard deviation was 3.7%

the transfer rate of prim-

-glucosylcimifugin was 66.8%-93.5%

the average metastasis rate was 79.4%

the standard deviation was 12.1%

the transfer rate of 4′-

-glucosyl-5-

-methylvisamminol was 70.4%-98.2%

the average transfer rate was 83.40%

the standard deviation of 10.8%. The results of various methodological studies were consistent with the requirements of RSD. The fingerprinting analysis was performed using similarity evaluation software for traditional Chinese medicine fingerprints

and 9 principal common peaks were identified. The similarity was higher than 0.9.

Conclusion:

The quality evaluation method established in this study has a good stability

precision and repeatability. The fingerprints have a high similarity

which is suitable for the quality evaluation of standard decoctions of Saposhnikoviae Radix pieces

and can provide the scientific basis for the quality evaluation of other related preparations.

关键词

防风标准汤剂质量标准指纹图谱

Keywords

Saposhnikoviae Radixstandard decoctionquality standardfingerprint

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