Objective:

2

To establish a supercritical fluid chromatography(SFC) method for separating and purifying costunolide and dehydrocostus lactone in Aucklandiae Radix.

Method:

2

With supercritical carbon dioxide as the mobile phase

the effect of six factors

such as type of chromatographic columns

modifiers and modifiers ratio

flow rate of mobile phase

pressure and temperature

on the separation process of supercritical fluid chromatography were explored. The target components were separated and prepared by semi-preparative supercritical fluid chromatography. High performance liquid chromatography and nuclear magnetic resonance were used to analyze the components and study the thermodynamic regularity of the chromatographic process.

Result:

2

C

18

column (10 mm×250 mm

5 μm) was adopted

with supercritical fluid dioxide as the mobile phase

the ratio of methanol was 0.13%

the flow rate was 12 mL·min

-1

column pressure was 13 MPa

column temperature was 318 ℃

and detection wavelength was 225 nm. The sample was injected for 20 times

crude extract was 4 mg

and each target component was collected according to the chromatogram. Its purity was determined to be more than 99%by HPLC

and its structure was determined as costunolide and dehydrocostus lactone by NMR. Under this condition

the SFC separation process was normal-phase chromatography.

Conclusion:

2

The method can be used to prepare effective components of Aucklandiae Radix with a high purity and low solvent residue.