Objective:

2

To explore the effect of Zingiberis Rhizoma Recens(ZRR) on hemorrhoids in mice and rats.

Method:

2

Sixty SPF-grade SD rats were divided into blank group

model group

Ma Yinglong Shexiang hemorrhoid ointment group (7.5 g·kg

-1

)

and large and small-dose ZRR paste groups (10

5 g·kg

-1

). ZRR paste was applied in Yongquan acupoint to observe the effect of 0.05 mL injection with 75% acetic acid solution on hemorrhoids induced by subcutaneous anus in rats

the levels of interleukin-1

β

(IL-1

β

)

interleukin-6 (IL-6)

tumor necrosis factor-

α

(TNF-

α

)

nitric oxide (NO) in the serum were detected by enzyme linked immunosorbent assay(ELISA)

and the rectal histopathology was detected by hematoxylin-eosin(HE). Sixty SPF-grade KM mice were divided into blank group

model group

MA Ying-long Shexiang hemorrhoid ointment group (7.5 g·kg

-1

)

and large and small-dose ginger essential oil groups (0.06

0.03 mL

three times a day). ZRR paste was applied in crissum to observe the effect of injection of 20% acetic acid solution 0.05 mL (maintaining for 1 min) on hemorrhoids in mice induced by anus. The degree of local swelling ulcer around the anus and score was observed

levels of IL-1

β

TNF-

α

in the serum were determined by ELISA

and the rectal histopathology was detected by HE staining.

Result:

2

In the experimental study on treating hemorrhoids with ZRR paste applied on Yongquan point of rats

compared with normal group

serum levels of IL-1

β

IL-6

TNF-

α

NO in model group were significantly higher (

P

<

0.01). The pathological injury of rectum was serious. Compared with model group

serum levels of IL-1

β

IL-6

TNF-

α

NO were decreased in each administration group (

P

<

0.05

P

<

0.01)

and the degree of rectal tissue injury was relieved (

P

<

0.01). In the experiment of external application of ZRR essential oil around the anus for treating hemorrhoids

compared with normal group

perianal swelling and severe ulcer injury were observed in the model group (

P

<

0.01). The serum levels of IL-1

β

TNF-

α

in model group were significantly higher (

P

<

0.01)

and the pathological injury of rectum was serious. Compared with model group

each administration group showed the alleviation in perianal pathological conditions (

P

<

0.01)

the reduction in serum IL-1

β

TNF-

α

levels (

P

<

0.01)

and the relief in the extent of rectal tissue injury (

P

<

0.01).

Conclusion:

2

External application of ZRR can effectively inhibit perianal swelling and ulcer degree

with a good therapeutic effect on hemorrhoids model in rats and mice.