Objective:

2

To investigate the therapeutic effect and related mechanism of Shenling Baizhu San on 3%dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice.

Method:

2

Sixty male SPF C57BL/6 mice were randomly divided into control group

salazosulfapyridine (SASP

0.52 g·kg

-1

)

and low

medium and high-dose (31.2

15.6

7.8 g·kg

-1

) Shenling Baizhu San groups. Except for the control group

mice in the other groups were given distilled water containing 3%dextran sulfate sodium salt for a week to establish UC models. The drug was administered once a day for 14 days. The normal group and the model group were administered with 0.9%physiological saline at 20 mL·kg

-1

. The mice's body weight

fecal traits

and occult blood were observed daily

and the disease activity index (DAI) was scored. After the end of the administration

the blood was collected

mice colons were collected

weighed and measured for length

and pathological sections were prepared. Enzyme-linked immunosorbent assay (ELISA) kit was used to detect the serum levels of interleukin-18 (IL-18) and IL-1

β

in mice; htoxylin eosin (HE) and alixin blue/schiff periodic acid shiff(AB/PAS) staining were used to observe the pathological changes of colon tissues; Western blot was used to detect the colon tissue of mice nucleotide binding oligomerization domain-like receptor 3 (NLPR3)

NLPR6 protein expression levels.

Result:

2

Compared with the normal group

the DAI score of the model group was increased (

P

<

0.01)

the colon weight was increased

and the length was shortened (

P

<

0.01); the pathological damage of the colonic mucosa was severe; the serum IL-1

β

content increased (

P

<

0.05); NLRP3 protein expression increased

while NLRP6 expression decreased (

P

<

0.01). Compared with the model group

the DAI scores of the SASP group and the high-dose group were decreased (

P

<

0.05)

the colon weight

length

and pathological conditions were improved; the serum IL-1

β

concentration was decreased (

P

<

0.05); NLRP3 protein expression decreased (

P

<

0.01)

and NLRP6 expression was up-regulated (

P

<

0.05

P

<

0.01).

Conclusion:

2

Shenling Baizhu San has the effect in treating DSS-induced UC mice

which may be related to the regulation of NLRP3

NLRP6 protein and related inflammatory factors

so as to reduce intestinal inflammation and alleviate intestinal mucosal damage.