针捕集法、静态顶空法和水蒸气蒸馏法结合GC-MS对紫花地丁挥发性成分的比较

秦艳; 翁静艳; 庞英明; 程志红

doi:10.13422/j.cnki.syfjx.20182120

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针捕集法、静态顶空法和水蒸气蒸馏法结合GC-MS对紫花地丁挥发性成分的比较

资源与质量评价 | 更新时间：2021-02-09

- 针捕集法、静态顶空法和水蒸气蒸馏法结合GC-MS对紫花地丁挥发性成分的比较
- Comparative Analysis of Volatile Organic Compounds in Viola yedoensis by Needle Trap,Static Headspace and Hydrodistillation Methods Coupled with GC-MS
- 中国实验方剂学杂志 2019年25卷第4期页码：153-161
- 作者机构：
  
  1.复旦大学　药学院，上海　 201203；
  2.复旦大学　附属中山医院，上海　 200032；
  3.弗科斯科技有限公司，上海　 200233
- 作者简介：
  
  秦艳，硕士，从事中药质量控制方法研究，E-mail：celeryan@163.com
  *程志红，博士，副研究员，从事中药药效物质基础与质量控制研究，Tel：021-51980157，E-mail：chengzhh@fudan.edu.cn
- 基金信息：
  
  国家自然科学基金面上项目(81073025;81473421)
- DOI：10.13422/j.cnki.syfjx.20182120
  中图分类号： R284.1;R931.6;R289;R22
- 纸质出版日期：2019-02-20，
  
  网络出版日期：2018-08-27，
  
  收稿日期：2018-03-23，
- 稿件说明：
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秦艳, 翁静艳, 庞英明, 等. 针捕集法、静态顶空法和水蒸气蒸馏法结合GC-MS对紫花地丁挥发性成分的比较[J]. 中国实验方剂学杂志, 2019,25(4):153-161.

Yan QIN, Jing-yan WENG, Ying-ming PANG, et al. Comparative Analysis of Volatile Organic Compounds in Viola yedoensis by Needle Trap,Static Headspace and Hydrodistillation Methods Coupled with GC-MS[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019,25(4):153-161.
秦艳, 翁静艳, 庞英明, 等. 针捕集法、静态顶空法和水蒸气蒸馏法结合GC-MS对紫花地丁挥发性成分的比较[J]. 中国实验方剂学杂志, 2019,25(4):153-161. DOI： 10.13422/j.cnki.syfjx.20182120.

Yan QIN, Jing-yan WENG, Ying-ming PANG, et al. Comparative Analysis of Volatile Organic Compounds in Viola yedoensis by Needle Trap,Static Headspace and Hydrodistillation Methods Coupled with GC-MS[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019,25(4):153-161. DOI： 10.13422/j.cnki.syfjx.20182120.

摘要

目的：

系统研究紫花地丁的挥发性成分，并比较针捕集法、静态顶空法和水蒸气蒸馏法在紫花地丁挥发性成分分析上的异同。

方法：

采用针捕集法、静态顶空法和水蒸气蒸馏法并结合GC-MS分别对紫花地丁的挥发性成分进行了分离和鉴定研究，首次使用填充了Tenax，Carbopack X和Carboxen 1000共3种吸附剂的捕集针，采用峰面积归一化法确定各成分的相对质量分数。

结果：

采用针捕集法从紫花地丁中共鉴定出挥发性成分112个，主要为中等挥发性的成分，以脂肪醛、酮、醇、单萜、倍半萜和芳香族类化合物为主。采用静态顶空法共从中鉴定出挥发性成分37个，主要为高挥发性成分；采用水蒸气蒸馏法共从中鉴定出挥发性成分78个，主要为低挥发性成分。采用这3种方法检出的共有挥发性成分只有13种。

结论：

该研究将为全面分析中药挥发性成分提供了新的研究方法，相对于静态顶空法和传统的水蒸气蒸馏法，针捕集法具有分析速度快、操作简单、富集效果好和灵敏度高等特点。这3种方法在分析挥发性成分类型上是互补的，不能相互替代。

Abstract

Objective:

To systemically investigate the volatile organic compounds (VOCs) of

Viola yedoensis

and to compare the VOCs differences of

yedoensis

obtained by the needle trap

static headspace and hydrostillation methods.

Method:

The needle trap

static headspace and hydrostillation methods coupled with gas chromatography-mass spectrometer (GC-MS) were developed for isolation and identification of the VOCs in

yedoensis

. The relative content of each component was obtained by peak area normalization with a triple-bed needle packed with Tenax

Carbopack X and Carboxen 1000 sorbents.

Result:

The 112 compounds were trapped by using needle trap

mainly moderate volatile components

including aldehydes

ketones

alcohols

monoterpenes

sesquiterpenoids and aromatic compounds. The static headspace and hydrodistillation methods were allowed to obtain 37 (mainly the high-volatile components) and 78 compounds (mainly low-volatile components)

respectively. Only 13 common volatile components were detected in all these three methods.

Conclusion:

The results clearly demonstrated that the needle trap method is an alternative method for sampling VOCs of herbs

characterized by fast analysis

simple operation

good enrichment effect and high sensitivity.These three methods for VOCs analysis are complementary for each other.

关键词

紫花地丁针捕集法静态顶空法水蒸气蒸馏法气相色谱-质谱联用技术

Keywords

Viola yedoensisneedle trapstatic headspacehydrostillationGC-MS

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