Objective:

2

To study the effect of Morindae Officinalis Radixe extract from different origins on the vascular growth of damaged internodes of zebra fish

so as to discuss regional differences of Morindae Officinalis Radix from different origins.

Method:

2

A zebra fish model of internode vascular injury induced by PTK787 was established to study the effect of Morindae Officinalis Radix extract from Guangdong

Guangxi and Fujian on the growth of internode vascular in zebra fish

and detect the number of internode angiogenesis in zebra fish treated with PTK787.On the basis of principal component analysis

relative angiogenesis rate was taken as the index

and the experimental data was analyzed by SPSS 17.0 statistical software.

Result:

2

The extract of Morindae Officinalis Radix had a repairing effect on the growth of damaged internode blood vessels of zebra fish

and the extracts of Morindae Officinalis Radix from Guangdong Gaoyao

and Guangdong Yunan

were better than those from other producing areas. The average number of internode angiogenesis was 9

the average length of angiogenesis was 448-504 μm

and the relative rate of angiogenesis was between 7.9% and 8.86%. The results of cluster analysis showed that Morindae Officinalis Radix of 12 different origins were classified into four categories

Guangdong Deqing

and Guangdong Gaoyao

Guangxi Cangwu and Guangxi Cenxi

and Guangdong Yunan

and Guangxi Cangwu could be better clustered.

Conclusion:

2

There are obvious regional differences in the pro-angiogenic effect of Morindae Officinalis Radix from different origins. Relevant studies provide basic data for the quality assessment of Morindae Officinalis Radix medicinal materials.