Objective:

2

To identify the chemical compositions and conduct relative content analysis of the essential oil from wild

Doellingeria scaber

in high altitude area of Dabie mountains

and investigate its antioxidant capacity.

Method:

2

The volatile oil of

D

.

scaber

was extracted by simultaneous distillation extraction (SDE) method. Its chemical compositions were analyzed by gas chromatography-mass spectometry (GC-MS)

and the relative percentage content of each composition was determined by peak area normalization method. The antioxidant activities of the oil were evaluated by reducing ability method

1

1-diphenyl-2-picrylhydrazyl (DPPH) method and

β

-carotene bleaching assay.

Result:

2

A total of 50 components representing 86.91% in this plant were identified. The main chemical compositions were (

E

)-

β

-farnesene(20.21%)

germacrene D(9.94%)

hexadecanoic acid(8.66%)

β

-terpinene(7.82%)

caryophyllene(6.9%) and

p

-cymen-8-ol(4.48%). The main components of volatile oils include alkenes (56.57%)

alcohols (12.24%)

fatty acids (11.24%)

epoxides(2.93%)

esters(1.82%)

aldehydes and ketones (1.60%)

and aromatics (0.51%). The reduction ability of volatile oil to iron ions increases with the increase of concentration

but the reduction ability is far weaker than that of vitamin C. The 50% inhibiting concentration (IC

50

) of volatile oil on DPPH free radicals and in

β

-carotene/linoleic acid lipid system was 0.72 g·L

-1

and 0.10 g·L

-1

respectively

indicating that the volatile oil had a certain scavenging capacity on DPPH free radicals and a good inhibition effect on lipid peroxidation.

Conclusion:

2

GC-MS was used to identify the chemical compositions of the volatile oil from

D

.

scaber

and antioxidant test in vitro showed that

D

.

scaber

had certain antioxidant capacity

providing experimental basis for its development and utilization.