Effect of Erxian Decoction on Cardiac Electrophysiology in Peri-menopausal Rats

Hui-hui XIE; Ying WANG; Shuo YANG; Ye-hong DONG; Li ZHANG; Si-min HUANG; Peng XU; Bo LIU

doi:10.13422/j.cnki.syfjx.20191601

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Effect of Erxian Decoction on Cardiac Electrophysiology in Peri-menopausal Rats

Pharmacology | 更新时间：2021-02-09

- Effect of Erxian Decoction on Cardiac Electrophysiology in Peri-menopausal Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 18, Pages: 43-48(2019)
- 作者机构：
  
  江西中医药大学　药学院，南昌　 330004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191601
  CLC： R2-0; R22; R285.5; R289
- Published：20 September 2019，
  
  Published Online：07 May 2019，
  
  Received：28 January 2019，
- 稿件说明：
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Hui-hui XIE, Ying WANG, Shuo YANG, et al. Effect of Erxian Decoction on Cardiac Electrophysiology in Peri-menopausal Rats. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(18):43-48(2019)
DOI：

Hui-hui XIE, Ying WANG, Shuo YANG, et al. Effect of Erxian Decoction on Cardiac Electrophysiology in Peri-menopausal Rats. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(18):43-48(2019) DOI： 10.13422/j.cnki.syfjx.20191601.

摘要

目的：

探讨二仙汤对围绝经期大鼠心脏电生理的作用及机制。

方法：

雌性SD大鼠，根据体质量随机分组，分为假手术组，模型组，戊酸雌二醇组(8×10

－4

g·kg

－1

·d

－1

)，二仙汤低、中、高剂量组(4，8，12 g·kg

－

－1

)。除假手术组外，其余各组大鼠均彻底摘除卵巢复制围绝经期大鼠模型。同时分别灌胃给药，假手术组和模型组则灌胃等量生理盐水，每天1次，连续80 d。以生物信号采集仪连接Ⅱ导联电极记录大鼠心电图，酶联免疫吸附测定(ELISA)检测大鼠血清中雌二醇(E

)的含量，苏木素-伊红(HE)染色观察大鼠子宫病理形态学变化，蛋白免疫印迹法(Western bot)检测大鼠心肌组织中雌激素

受体(ER

)蛋白的表达。

结果：

与假手术组比较，模型组大鼠心电图P波，R波，T波幅度显著减小(

0.01)，PR间期，QRS时程，QT间期不同程度的缩短，大鼠血清中E

水平显著降低(

0.01)；大鼠子宫明显干瘪，皱缩，子宫内膜层变薄，腺体数量明显减少；心肌组织ER

受体蛋白表达显著降低(

0.01)。与模型组比较，二仙汤各剂量组均能改善上述大鼠子宫的病理变化，中、高剂量二仙汤能显著升高E

水平(

0.01)，显著逆转上述子宫的病理变化，上调ER

受体蛋白的表达(

0.01)。

结论：

二仙汤能改善围绝经期大鼠的心脏电生理变化，其机制可能与提高大鼠体内雌激素活性和雌激素受体表达有关。

Abstract

Objective:

To explore the effect and mechanism of Erxian decoction on peri-menopausal cardiac electrophysiology in rats.

Method:

Female sprague-dawley rats were randomly divided into six groups: sham operation group

model group

estradiol valerate group

and low

medium and high-dose Erxian decoction groups. Except the sham operation group

the rats in the other groups were completely removed from the ovarian replication peri-menopausal rat model. At the same time

estradiol valerate group (8×10

-4

g·kg

-1

·d

-1

)

low-dose Erxian decoction group (4 g·kg

-1

·d

-1

)

middle-dose Erxian decoction group (8 g·kg

-1

·d

-1

) and high-dose Erxian decoction group (12 g·kg

-1

·d

-1

)

sham operation group and model group were given the same amount of normal saline. The administration was given once a day for 80 consecutive days. The electrocardiogram of rats was recorded by biosignal detector connected to lead Ⅱ electrode. The content of estradiol (E

) in rat serum was detected by enzyme-linked immuno sorbent assay (ELISA). The pathological changes of rat uterus were observed by hematoxylin-eosin. The expression of the estrogen alpha receptor (ER

) protein was detected by Western blot method in myocardial tissue of rat.

Result:

Compared with the sham operation group

the amplitudes of P wave

R wave and T wave in the electrocardiogram of the model group were significantly decreased (

0.01)

and the PR interval

QRS time course and QT interval were shortened to different degrees. The E

level was significantly lower (

0.01). The uterus was obviously shriveled and shrank

the endometrial layer became thinner

and the number of glands reduced significantly. The expression of ER

receptor protein in myocardial tissue was significantly decreased (

0.01). Compared with the model group

the Erxian decoction group could improve the pathological changes of ECG in the above rats. The medium and high-dose Erxian decoction can significantly increase the E

level (

0.01)

reverse significantly the pathological changes of the uterus

and up-regulate the expression of ER

receptor protein (

0.01).

Conclusion:

Erxian decoction can improve cardiac electrophysiological changes in peri-menopausal rats

and its mechanism may be related to the increase of estrogen activity and estrogen receptor expression in rats.

关键词

二仙汤围绝经期雌激素心脏电生理

Keywords

Erxian decoctionperi-menopauseestrogencardiac electrophysiology

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