Effect of Oxymatrine in Inhibiting Proliferation and Migration of HT-29 Cells Induced by Insulin

Di PAN; Wen ZHANG; Rong ZHANG; Shi-quan GAN; Yan CHEN; Nen-ling ZHANG; Yi-ni XU; Xiang-chun SHEN

doi:10.13422/j.cnki.syfjx.20192022

您当前的位置：

首页 >

文章列表页 >

Effect of Oxymatrine in Inhibiting Proliferation and Migration of HT-29 Cells Induced by Insulin

Pharmacology | 更新时间：2021-02-09

- Effect of Oxymatrine in Inhibiting Proliferation and Migration of HT-29 Cells Induced by Insulin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 24, Pages: 36-42(2019)
- 作者机构：
  
  1.贵州省天然药物资源高效利用工程中心，贵阳　 550025；
  2.贵州医科大学　天然药物资源优效利用重点实验室，贵阳　 550025
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192022
  CLC： R22; R242; R2-031; R285.5
- Published：20 December 2019，
  
  Published Online：24 June 2019，
  
  Received：27 January 2019，
- 稿件说明：
扫描看全文
Di PAN, Wen ZHANG, Rong ZHANG, et al. Effect of Oxymatrine in Inhibiting Proliferation and Migration of HT-29 Cells Induced by Insulin. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(24):36-42(2019)
DOI：

Di PAN, Wen ZHANG, Rong ZHANG, et al. Effect of Oxymatrine in Inhibiting Proliferation and Migration of HT-29 Cells Induced by Insulin. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(24):36-42(2019) DOI： 10.13422/j.cnki.syfjx.20192022.

摘要

目的：

利用胰岛素培养人结肠癌细胞HT-29，体外模拟糖尿病患者体循环中高胰岛素环境，研究氧化苦参碱(OMT)对胰岛素诱导的人结肠癌HT-29细胞增殖和迁移的影响。

方法：

采用噻唑蓝(MTT)比色法和平板克隆实验检测OMT(2

8 mmol·L

－1

)对胰岛素诱导HT-29的增殖作用，倒置显微镜观察该模型下细胞形态的变化；通过划痕修复实验评价OMT对胰岛素诱导的HT-29迁移能力，流式细胞术Annexin V/碘化丙啶(PI)双染实验检测该模型中HT-29细胞周期与凋亡的变化；蛋白免疫印迹法(Western blot)检测细胞周期相关蛋白的表达以及细胞迁移相关蛋白的表达。

结果：

MTT比色法、平板克隆及划痕修复实验均显示，与空白组比较，胰岛素能促进人结肠癌HT-29细胞的增殖和迁移(

0.05)；选择2

8 mmol·L

－1

OMT处理细胞24 h，与胰岛素组比较，OMT 4

8 mmol·L

－1

明显抑制其增殖作用(

0.05)，可诱导HT-29细胞发生G

期阻滞(

0.05)，8 mmol·L

－1

OMT下调细胞周期蛋白D

(CyclinD

)和周期蛋白依赖性激酶4(CDK4)表达(

0.05)，上调周期负调节蛋白p27水平(

0.05)；此外，划痕修复实验结果显示，与胰岛素组比较，OMT各浓度组均能抑制胰岛素诱导的细胞迁移作用(

0.05)，其机制可能与增加黏附蛋白(E-cadherin)(

0.05)和降低波形蛋白(Vimentin)(

0.05)相关。

结论：

OMT能抑制胰岛素诱导的人结肠癌HT-29细胞增殖及迁移作用，其作用机制可能与调控细胞周期及迁移相关蛋白有关。

Abstract

Objective:

To investigate the effect of oxymatrine (OMT) on the proliferation and migration of human colon cancer cell line HT-29 under Type Ⅱ diabetes environment by co-culturing HT-29 with insulin to simulate hyperinsulinemia.

Method:

The effect of OMT (2

8 mmol·L

-1

) on insulin-induced proliferation of HT-29 was detected by methyl thiazolyl tetrazolium (MTT) assay and cloning assay. The morphology change and cell migration were evaluated under microscope and by wound healing assay. The Annexin V/propidium iodide(PI) assay was used to detect the change of insulin-induced HT-29 cell cycle and apoptosis. Western blot was performed to validate the expression of cell cycle-related protein and cell migration protein.

Result:

Insulin significantly increased growth of HT-29 (

0.05). Compared with insulin group

OMT with 2

8 mmol·L

-1

showed a significant inhibitory effect in this model (

0.05). In addition

OMT blocked HT-29 cell cycle in G

phase (

0.05)

and showed a slight apoptotic effect. Western blot showed that the down-regulation of Cyclin D

CDK4 and the up-regulation of p27 by OMT might involve the growth inhibition mechanism. Furthermore

OMT reduced the migration of insulin-induced HT-29 according to wound healing assay(

0.05). Decreased Vimentin (

0.05)and increased E-cadherin(

0.05)might be correlated with the migration restrain.

Conclusion:

OMT can inhibit the proliferation and migration of insulin-induced HT-29 cells. The changes of cell cycle and migration related proteins may be correlated with the mechanism.

关键词

HT-29细胞氧化苦参碱胰岛素细胞增殖细胞迁移

Keywords

HT-29 celloxymatrineinsulincell proliferationcell migration

references

MENG X, SAN C L, ZHI P X, et al. Noninvasive DNA methylation biomarkers in colorectal cancer: a systematic review [J].J Dig Dis, 2015, 16(12): 699-712.

何祖坤，苏丹，珏宁君，等．长链非编码RNA在结直肠癌中的研究进展[J]．实用医学杂志，2018，34(4)：665-668．

Ashamalla M, Youssef I, Yacoub M, et al. Obesity, diabetes and gastrointestinal malignancy: the role of metformin and other anti-diabetic therapy [J].Glob J Obes Diabetes Metab Syndr, 2018, 5(2): 8-14.

Currie C J, Poole C D, Gale E A. The influence of glucose-lowering therapies on cancer risk in type 2 diabetes [J].Diabetologia, 2009, 52(9): 1766-1777.

刘家旋，剧永乐，唐诗彬，等．高胰岛素血症与结直肠癌发生、发展的相关性研究[J]．泰山医学院学报，2017，38(7)：728-730．

陈妍，温佳敏，徐旖旎，等．氧化苦参碱对醛固酮诱导心肌细胞损伤的保护作用[J]．中国实验方剂学杂志，2016，22(19)：76-80．

杨李强，孙爱华，徐旖旎，等．氧化苦参碱通过调控MAPK信息通路改善醛固酮诱导的心肌细胞损伤[J]．中国实验方剂学杂志，2017，23(15)：130-135．

张彦燕，王羿，徐旖旎，等．氧化苦参碱对TGF-β1诱导心肌细胞损伤的保护作用[J]．中国实验方剂学杂志，2017，23(17)：149-153．

Singh S, Earle C C, Bae S J, et al. Incidence of diabetes in colorectal cancer survivors [J].J Natl Cancer Inst, 2016, 108 (6): 1-7.

杨俊奎．氧化苦参碱联合乌司他丁治疗老年重症急性胰腺炎的疗效分析[J]．中国医药指南，2018，16(5)：200-201．

吴琴，高云．氧化苦参碱药理作用的分子机制研究进展[J]．中国药理学通报，2015，31(6)：759-762．

刘艳明，王雪芳．氧化苦参碱对缺血缺氧致兔心律失常保护作用及机制研究[J]．河北医药，2015，37(9)：1308-1310．

韩华，曹苋恺，殷鑫，等．氧化苦参碱抗肿瘤作用机制的研究进展[J]．现代中药研究与实践，2017，31(2)：78-81．

易云，吴琴，黄莉萍，等．氧化苦参碱对高糖毒性下血管内皮细胞的保护作用[J]．中国药理学通报，2015，31(4)：499-503．

杜亚萍，宋光耀，王富军，等．氧化苦参碱对胰岛素抵抗的影响及其作用机制[J]．世界华人消化杂志，2015，23(16)：2555-2561．

WANG S B, JIA J P. Oxymatrine attenuates diabetes-associated cognitive deficits in rats [J].Acta Pharmacol Sin, 2014, 35 (3): 331-338.

Wiela H A, Kowalska T, Filipczyk C E, et al. Evaluation of the toxicity of anticancer chemotherapy in patients with colon cancer [J].Adv Clin Exp Med, 2015, 24 (1): 103-111.

殷雪芹．金芪降糖片联合吡格列酮片治疗2型糖尿病高胰岛素血症的临床疗效[J]．海峡药学，2016，28(2)：107-108．

张欣霞，陈婷，涂盛，等．胰岛素信号通路与肿瘤风险相关性的研究进展[J]．浙江中医药大学学报，2015，39(5)：418-422．

van Horssen R, Freire Jorge P, van Dam G M, et al. The Warburg effect and its role in tumor metabolism: opportunities for new cancer treatments [J].Ned Tijdschr Geneeskd, 2015, 160: 9489-9497.

向永佳．氧化苦参碱诱导人结肠癌SW620细胞周期阻滞的作用机制研究[J]．中国药业，2015，24(1)：17-18．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Anti-tumor Effect of Oxymatrine on Non-small Cell Lung Cancer Cell Lines and Its Mechanism

Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells

Effect of Sijunzi Tang in Promoting Migration and Proliferation of Small Intestinal Epithelial Cells by Up-Regulating c-Myc Expression

Analysis of Mechanism of Antidepressant Effect of Sophora flavescens Seed Extract

Chinese Medicine Regulates Signaling Pathways Related to Proliferation and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells： A Review

Related Author

Jiao LUO

Wei XIE

Kun-chi ZHANG

Jin WANG

Li ZHOU

Xing-kui TAO

Xing-tao ZHANG

Hai-chao WANG

Related Institution

Guizhou Engineering Vocational College

School of Pharmacy， Shanghai University of Medicine and Health Sciences

Shanghai Key Laboratory for Molecular Imaging，Shanghai University of Medicine & Health Sciences

Zhoupu Hospital Affiliated to Shanghai University of Medicine & Health Sciences

School of Clinical Medicine，Guizhou Medical University

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰