Quantitative Determination of Four Active Constituents in Anti-inflammatory and Analgesic Active Fraction of Gaultheria leucocarpa var. yunnanensis

Xiao LI; Xiu-huan WANG; Yi-cheng ZHAO; Le WANG; Shu-sheng FAN; Xiao-ping WANG; Xiao XU; Xin YAN; Ting HE; Yan-li CHANG; Gai-mei SHE

doi:10.13422/j.cnki.syfjx.20192111

您当前的位置：

首页 >

文章列表页 >

Quantitative Determination of Four Active Constituents in Anti-inflammatory and Analgesic Active Fraction of Gaultheria leucocarpa var. yunnanensis

Resource and Quality Evaluation | 更新时间：2021-02-09

- Quantitative Determination of Four Active Constituents in Anti-inflammatory and Analgesic Active Fraction of Gaultheria leucocarpa var. yunnanensis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 24, Pages: 91-96(2019)
- 作者机构：
  
  北京中医药大学　中药学院，北京　 102488
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192111
  CLC： R284.2; R289; R22; R2-031
- Published：20 December 2019，
  
  Published Online：19 July 2019，
  
  Received：22 January 2019，
- 稿件说明：
扫描看全文
Xiao LI, Xiu-huan WANG, Yi-cheng ZHAO, et al. Quantitative Determination of Four Active Constituents in Anti-inflammatory and Analgesic Active Fraction of Gaultheria leucocarpa var. yunnanensis. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(24):91-96(2019)
DOI：

Xiao LI, Xiu-huan WANG, Yi-cheng ZHAO, et al. Quantitative Determination of Four Active Constituents in Anti-inflammatory and Analgesic Active Fraction of Gaultheria leucocarpa var. yunnanensis. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(24):91-96(2019) DOI： 10.13422/j.cnki.syfjx.20192111.

摘要

目的：

建立高效液相色谱法(HPLC)测定民族药滇白珠抗炎镇痛活性部位(ARF)中4种活性成分methyl benzoate-2-

-xylopyranosyl(1-2)[

-xylopyranosyl(1-6)]-

-glucopyranoside(MSTG-A)，methyl benzoate-2-

-glucopyranosyl(1-2) [

-xylopyranosyl(1-6)]-

-glucopyranoside(MSTG-B)，gaultherin及绿原酸的含量测定方法，为滇白珠药材的深入研究及其质量控制提供方法依据，并为后期滇白珠的制剂与临床应用奠定基础。

方法：

采用COSMOSIL 5C

-PAQ色谱柱(4.6 mm×250 mm，5 μm)，以甲醇-0.2%冰乙酸水溶液为流动相梯度洗脱，柱温25 ℃，流速1 mL·min

－1

，检测波长294 nm。

结果：

MSTG-A，MSTG-B，gaultherin，绿原酸分别在0.014 06～0.450 00，0.007 81～0.250 00，0.003 13～0.100 00，0.000 94～0.030 00 g·L

－1

线性关系良好(

≥0.999 7)；方法精密度、重复性、稳定性良好；平均加样回收率分别在100.81%，99.19%，96.12%，102.56%，其RSD分别为1.4%，0.7%，0.7%，2.4%。滇白珠ARF部位中MSTG-B，MSTG-A，gaultherin，绿原酸质量分数分别为23.608，41.973，8.282，2.673 mg·g

－1

。

结论：

该方法操作简便、准确、重复性好，适用于滇白珠抗炎镇痛活性部位中4种活性成分的含量测定，为滇白珠药材的深入研究、质量控制与综合评价提供参考依据，并为后期滇白珠药材的制剂与临床应用奠定基础。

Abstract

Objective:

To establish a high performance liquid chromatography (HPLC) method for determination of four active constituents

namely MSTG-A

MSTG-B

gaultherin and chlorogenic acid in the anti-inflammatory and analgesic active fraction (ARF) of the ethnic medicine

Gaultheria leucocarpa

var.

yunnanensis

in order to provide a methodological basis for the in-depth study and quality control of

leucocarpa

var.

yunnanensis

and lay a foundation for later preparation and clinical application.

Method:

The determination was performed on COSMOSIL 5C

-PAQ (4.6 mm×250 mm

5 μm) column with methanol-0.2%glacial acetic acid (gradient elution) as the mobile phase at a flow rate of 1 mL·min

-1

. The column temperature was 25 ℃. The detection wavelength was set at 294 nm.

Result:

The linear range of MSTG-B

MSTG-A

gaultherin and chlorogenic acid were 0.014 06-0.450 00

0.007 81-0.250 00

0.003 13-0.100 00

0.000 94-0.030 00 g·L

-1

(

≥0.999 7)

respectively

with a good precision

repeatability and stability. And the average recoveries were 100.81%

98.99%

96.12%and 102.56%

respectively. RSDs were 1.4%

0.7%

2.4%

respectively. The contents of MSTG-B

MSTG-A

gaultherin and chlorogenic acid in ARF fraction of

leucocarpa

var.

yunnanensis

were 23.608

41.973

8.282

2.673 mg·g

-1

respectively.

Conclusion:

The established method was simple and accurate

with a high repeatability. It can be used for determination of four active constituents in ARF fraction of

leucocarpa

var.

yunnanensis

so as to provide a reference for the in-depth research

quality control and comprehensive evaluation of

leucocarpa

var.

yunnanensis

and lay a solid foundation for preparation and clinical application.

关键词

滇白珠活性部位MSTG-AMSTG-Bgaultherin绿原酸含量测定

Keywords

Gaultheria leucocarpa var. yunnanensisactive fractionMSTG-AMSTG-Bgaultherinchlorogenic acidquantitative determination

references

中国科学院昆明植物研究所．云南植物志．4卷[M]．北京：科学出版社，1986：5．

马小军，郑俊华，陈新滋．民族药滇白珠资源研究[J]．中国中药杂志，2001，26(2)：85-89．

李东宸，折改梅，万钦黎．民族药——抗风湿滇白珠的研究进展[C]//2010施慧达杯第十届全国青年药学工作者最新科研成果交流会论文集，长春：出版社不详，2010：43-52．

宋志钊，刘元．HPLC测定滇白珠中槲皮苷的含量[J]．中国实验方剂学杂志，2012，18(13)：99-101．

熊玉兰，肖冰，马小军，等．滇白珠抗风湿性关节炎活性成分研究[J]．中国中药杂志，2009，34(19)：2516-2519．

陈应康，佘福强，刘大腾，等．苗药透骨香抗急性痛风性关节炎作用的实验研究[J]．中药材，2016，39(9)：2118-2121．

黄超，张丹，辛文妤，等．水杨酸甲酯糖苷抗大鼠急性胸膜炎的作用研究[J]．中国药理学通报，2013，29(3)：328-332．

张治针，果德安，李长龄，等．滇白珠抗菌抗炎和镇痛活性的实验研究[J]．西北药学杂志，1999，14(2)：60-61．

李东宸，郭志琴，吕海宁，等．民族药滇白珠的体外抗氧化活性研究[J]．中医药学报，2010，38(6)：62-66．

陈娟，孟祥武，张朝贵，等．滇白珠糖浆对老年眩晕及脑血流的影响[J]．中国老年学杂志，2016，36(9)：2156-2158．

何飞，韦桂宁，苏华，等．滇白珠提取物对胃肠功能以及止泻作用研究[J]．中医药导报，2016，22(9)：20-23．

XIE M, LU Y, YAN C, et al. The anti-rheumatoid arthritis property of the folk medicine Dianbaizhu (Gaultheria leucocarpa var. yunnanensis, Ericaceae) [J].Nat Prod Commun, 2014, 9(12): 1773-1776.

熊玉兰，肖冰，马小军，等．滇白珠抗风湿性关节炎活性成分研究[J]．中国中药杂志，2009，34(19)：2516-2519．

赵怡程，何婷，翁稚颖，等．一种滇白珠富含水杨酸甲酯糖苷抗炎镇痛活性部位提取富集方法的探索[J]．国际药学研究杂志，2017，44(9)：884-889．

何婷，赵怡程，李鹏跃，等．滇白珠抗炎镇痛活性部位的化学成分研究[J]．中草药，2017，48(17)：3469-3474．

ZHANG B, HE X L, DING Y, et al. Gaultherin, a natural salicylate derivative from Gaultheria yunnanensis: towards a better non-steroidal anti-inflammatory drug [J].Eur J Pharmacol, 2006, 530(1/2): 166-171.

ZHANG B, LI J B, ZHANG D M, et al. Analgesic and anti-inflammatory activities of a fraction rich in gaultherin isolated from Gaultheria yunnanensis (Franch.) Rehder [J].Biol Pharm Bull, 2007, 30(3): 465-469.

ZHANG D, LIU R, SUN L, et al. Anti-inflammatory activity of methyl salicylate glycosides isolated from Gaultheria yunnanensis (Franch.) Rehder [J].Molecules, 2011, 16(5): 3875-3884.

Dos Santos M D, Almeida M C, Lopes N P, et al. Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid [J].Biol Pharm Bull, 2006, 29(11): 2236-2240.

Azza E M, Bassiouni Y, Mahmoud K, et al. Chlorogenic acid as potential anti-inflammatory analgesic agent: an investigation of the possible role of nitrogen-based radicals in rats [J].Int J Pharm Toxicol Sci, 2011, 1: 24-33.

张斌．药物代谢动力学性质评价在新药发现及开发中的应用研究[D]．北京：中国协和医科大学，2007．

DU G H, ZHANG D M, ZHANG B, et al. The synthesis method and usage of methyl salicylate lactose glycosides compounds, China: 200910082224.0[P].2010-10-20.

LAN X, LIU R, SUN L, et al. Methyl salicylate-2-O-β-D-lactoside, a novel salicylic acid analogue, acts as an anti-inflammatory agent on microglia and astrocytes [J].J Neuro inflamm, 2011, 8(1): 98.

ZHANG T T, SUN L, LIU R, et al. A novel naturally occurring salicylic acid analogue acts as an anti-inflammatory agent by inhibiting nuclear factor-kappa B activity in RAW264.7 macrophages [J].Mol Pharm, 2012, 9(3): 671-677.

Tegeder I, Pfeilschifter J, Geisslinger G. Cyclooxygenase-independent actions of cyclooxygenase inhibitors [J].Faseb J, 2001, 15(12): 2057-2072.

WU K K. Aspirin andsalicylate: an old remedy with a new twist [J].Circulation, 2000, 102(17): 2022-2023.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Quality Evaluation and Suggestions on Pharmacopoeia Standard of Eucommiae Folium

Analysis on Chemical Constituents and Dynamic Accumulation of Main Components in Arctium lappa Leaves

Metabonomics Analysis on Different Varieties of Angelica sinensis Based on UPLC-Q-TOF-MS

Reaction of Chlorogenic Acid with Sulfite and Its Application in Identification of Sulfur-fumigated Herbs

Content Changes of Eight Components in Tetrastigmatis Hemsleyani Radix of Different Months

Related Author

WANG Ping

LIANG Shaojia

ZHANG Wenwen

FENG Weihong

YI Hong

LI Chun

GUO Fengqian

SI Qin

Related Institution

Institute of Traditional Chinese Medicine（TCM），Shanghai University of TCM

Yanling DU Renkang Biotechnology Co. Ltd.

Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences

Henan University of Chinese Medicine

Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province， Henan University of Chinese Medicine

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰