Objective:

2

To develop a method to quantify nine constituents in different medicinal parts of

Pimpinella thellungiana

in order to compare the content difference of the nine constituents

namely protocatechuic acid

gallic acid

neochlorogenic acid

chlorogenic acid

cryptochlorogenic acid

luteolin-7-

O

-

β

-

D

-glucuronide

isochlorogenic acid A

apigenin-7-

O

-

β

-

D

-glucuronide and isochlorogenic acid C.

Method:

2

The analysis was performed on an Agilent TC-C

18

column (4.6 mm×250 mm

5 μm) with acetonitrile and mixed acid solution (0.1%phosphoric acid-0.1%glacial acetic acid) as mobile phase for gradient elution. The handover detection wavelengths were at 265 and 325 nm. The column temperature was 20 ℃

and the flow rate was 1.0 mL·min

-1

. The experiment data was analyzed using the software of Markerlynx XS.

Result:

2

The nine constituents of protocatechuic acid

gallic acid

neochlorogenic acid

chlorogenic acid

cryptochlorogenic acid

luteolin-7-

O

-

β

-

D

-glucuronide

isochlorogenic acid A

apigenin-7-

O

-

β

-

D

-glucuronide and isochlorogenic acid C had a good degree of separation and a good linearity in their respective linear ranges(

r

>

0.999 8). The average recoveries ranged from 99.11%to 100.76%

and the RSD ranged from 0.9%to 2.0%. The results showed that the contents of the nine constituents had significant differences in different medicinal parts of

P

.

thellungiana

. The average contents of the nine constituents were the highest in leaves

which was followed by stem

and the lowest was in root.

Conclusion:

2

The study could provide evidence for the quality control

clinical application

and scientific resources utilization of

P

.

thellungiana

.