Comparative Study on Content of Medicinal Materials from Three Varieties of Sambucus williamsii

Dan-dan SUN; Jun-xiu YAO; Gang WANG; Huan-xiang HE; Xian-chen LIN; Qing-mei GUO; De-jun WU

doi:10.13422/j.cnki.syfjx.20191912

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Comparative Study on Content of Medicinal Materials from Three Varieties of Sambucus williamsii

Resource and Quality Evaluation | 更新时间：2021-02-09

- Comparative Study on Content of Medicinal Materials from Three Varieties of Sambucus williamsii
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 24, Pages: 124-130(2019)
- 作者机构：
  
  1.山东中医药大学，济南　 250355；
  2.山东省林业科学研究院，济南　 250014；
  3.平阴县林业局，济南　 250400
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191912
  CLC： R284.1; R285; R22; R2-031
- Published：20 December 2019，
  
  Published Online：17 June 2019，
  
  Received：12 December 2018，
- 稿件说明：
扫描看全文
Dan-dan SUN, Jun-xiu YAO, Gang WANG, et al. Comparative Study on Content of Medicinal Materials from Three Varieties of Sambucus williamsii. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(24):124-130(2019)
DOI：

Dan-dan SUN, Jun-xiu YAO, Gang WANG, et al. Comparative Study on Content of Medicinal Materials from Three Varieties of Sambucus williamsii. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(24):124-130(2019) DOI： 10.13422/j.cnki.syfjx.20191912.

摘要

目的：

测定接骨木(耐盐碱品种、天然红1号品种、青州15号品种)根皮、茎、叶、果实的活性成分含量，并对其化学成分的量进行化学模式识别。

方法：

采用紫外分光光度法(UV)测定多糖、黄酮、酚酸、三萜含量；采用pH示差法测定花青素含量；采用高效液相色谱法(HPLC)测定熊果酸、齐墩果酸、没食子酸、芦丁、槲皮素、原儿茶酸、莫诺苷含量。并采用化学模式识别的方法对3个品种接骨木的根皮、茎、叶、果实中的含量进行分析。

结果：

通过主成分分析中的

，

综合评分，进一步评价不同样品质量，结果发现，根皮和叶在综合排名中较靠前，其结果与其定量测定结果基本吻合。天然红1号品种根皮多糖和莫诺苷质量分数最高，分别为161.09，8.33 mg·g

－1

；黄酮、芦丁、槲皮素在天然红1号品种叶中质量分数最高，分别为71.93，4.09，3.57 mg·g

－1

；三萜在天然红1号品种果实中质量分数最高，为47.84 mg·g

－1

；酚酸和花青素在耐盐碱品种果实中质量分数最高，分别为11.25，94.32 mg·g

－1

；耐盐碱品种茎中齐墩果酸质量分数最高，为1.41 mg·g

－1

；耐盐碱品种叶中熊果酸、没食子酸质量分数最高，分别为2.12，0.34 mg·g

－1

；青州15号品种根皮中原儿茶酸质量分数最高，为0.12 mg·g

－1

。

结论：

天然红1号品种含量较其他两个品种高，质量好。

Abstract

Objective:

To determine the content of active ingredients in root bark

stems

leaves and fruits of three varieties of

Sambucus williamsii

(Saline-toleratedvariety

Natural Red No. 1 variety

Qingzhou No.15 variety)

and identify the chemical patterns of the chemical components.

Method:

The content of polysaccharides

flavonoids

phenolic acids and triterpene were determined by UV spectrophotometry (UV) colorimetry. The content of anthocyanin was determined by pH differential method. Ursolic acid

oleanolic acid

quercetin

protocatechuic acid

morroniside content were determined by high performance liquid chromatography (HPLC). The root bark

stems

leaves and fruits of three varieties were analyzed by chemical pattern recognition.

Result:

The quality of different samples was further evaluated by

and

comprehensive scores in principal component analysis. It was found that the root bark and leaves were higher in the comprehensive ranking

and the results were basically consistent with the quantitative results. The contents of polysaccharide and morroniside in Natural Red No. 1 variety root bark were the highest

which were 161.09 mg·g

-1

and 8.33 mg·g

-1

respectively. Flavonoids

rutin and quercetin were the highest in the Natural Red No. 1 variety leaf

which were 71.93

4.09 and 3.57 mg·g

-1

respectively. Triterpene was the highest in Natural Red No. 1 variety fruit

which was 47.84 mg·g

-1

. Phenolic acid and anthocyanin were the highest in Saline-tolerated variety fruits

which were 11.25 mg·g

-1

and 94.32 mg·g

-1

respectively. The Saline-tolerated variety stem had the highest oleanolic acid content

which was 1.41 mg·g

-1

. Saline-tolerated variety leaf had the highest contents of ursolic acid and gallic acid

which were 2.12 and 0.34 mg·g

-1

respectively. The highest content of protocatechuic acid in Qingzhou No. 15 variety root bark

which was 0.12 mg·g

-1

Conclusion:

The content of Natural Red No. 1 variety is higher than that of the other two varieties

with a good quality.

关键词

接骨木耐盐碱品种天然红1号品种青州15号品种根皮茎叶果实

Keywords

Sambucus williamsiiSaline-toleratedvarietyNatural Red No. 1varietyQingzhou No.15 varietyroot barkstemsleavesfruits

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