黄芪葛根配伍调节糖尿病大鼠血糖血脂炎症的作用与机制

刘倩; 范颖; 李新; 刘丽; 李军; 林庶茹; 訾慧

doi:10.13422/j.cnki.syfjx.20180941

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黄芪葛根配伍调节糖尿病大鼠血糖血脂炎症的作用与机制

药理 | 更新时间：2024-01-04

- 黄芪葛根配伍调节糖尿病大鼠血糖血脂炎症的作用与机制
- Effect and Mechanism of Astragali Radix-Puerariae Lobatae Radix Compatibility on Glucose-lipid and Inflammatory of Diabetes Rats
- 中国实验方剂学杂志 2018年24卷第11期页码：81-86
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81273653）
- DOI：10.13422/j.cnki.syfjx.20180941
  中图分类号： R285.5
- 纸质出版：2018
- 稿件说明：
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刘倩, 范颖, 李新, 等. 黄芪葛根配伍调节糖尿病大鼠血糖血脂炎症的作用与机制[J]. 中国实验方剂学杂志, 2018,24(11):81-86.

LIU Qian, FAN Ying, LI Xin, et al. Effect and Mechanism of Astragali Radix-Puerariae Lobatae Radix Compatibility on Glucose-lipid and Inflammatory of Diabetes Rats[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24(11): 81-86.
刘倩, 范颖, 李新, 等. 黄芪葛根配伍调节糖尿病大鼠血糖血脂炎症的作用与机制[J]. 中国实验方剂学杂志, 2018,24(11):81-86. DOI： 10.13422/j.cnki.syfjx.20180941.

LIU Qian, FAN Ying, LI Xin, et al. Effect and Mechanism of Astragali Radix-Puerariae Lobatae Radix Compatibility on Glucose-lipid and Inflammatory of Diabetes Rats[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24(11): 81-86. DOI： 10.13422/j.cnki.syfjx.20180941.

摘要

目的：考察黄芪葛根配伍对糖尿病大鼠血糖、血脂及炎症的调节作用与机制，分析黄芪葛根配伍在降糖降脂抗炎过程中的交互作用。方法：66只大鼠随机分为6组，除正常组外，其余各组采用链脲佐菌素（STZ）诱导糖尿病模型。正常组、模型组灌服蒸馏水（10 mL·kg-1），阳性药组灌服金芪降糖片混悬液（1.47 g·kg-1），黄芪组、葛根组、黄芪葛根汤组分别灌服黄芪水煎液（2.7，1.35，4.05 g·kg-1），连续给药30 d后处死。酶联免疫吸附试验法（ELISA）检测肝组织胰岛素（Ins），胰岛素受体（InsR），肿瘤坏死因子-α（TNF-α）水平；采用实时荧光定量聚合酶链式反应法（Real-time PCR）检测肝组织脂联素受体1（AdipoR1），腺苷酸活化蛋白激酶（AMPK），葡萄糖转运体-4（GLUT-4），过氧化物酶增殖体激活受体α（PPARα），脂肪酸转运蛋白4（FATP4），p38丝裂原活化蛋白激酶（p38 MAPK） mRNA相对表达量。结果：与模型组比较，黄芪、葛根及其配伍（黄芪葛根汤）能升高糖尿病大鼠肝脏Ins（葛根组除外），InsR水平，增加AdipoR1，AMPK（葛根组除外），GLUT-4，PPARα，FATP4 mRNA表达，降低TNF-α（葛根组除外）水平，减少p38 MAPK mRNA表达（P<0.05）。在调节InsR，PPARα mRNA，TNF-α水平时，单用黄芪作用比两药合用效果好。结论：黄芪葛根配伍在调节血糖血脂炎症过程中各有侧重，其机制与升高肝脏Ins水平、增加GLUT-4，FATP4 mRNA，降低p38 MAPK mRNA有关。

Abstract

Objective: To explore the regulatory effect and mechanism of Astragali Radix-Puerariae Lobatae Radix compatibility on glucose-lipid and inflammation in diabetic rats and analyze their interaction in glucose and lipid lowering and anti-inflammatory process. Method: Total 66 rats were randomly divided into 6 groups. Except the normal group

diabetes model was induced by intraperitoneal injection of streptozotocin (STZ) in other groups. Distilled water (10 mL·kg-1) was given in normal group and model group;Jinqi Jiangtangpian suspension (1.47 g·kg-1) was given in positive control group;Astragali Radix (2.7 g·kg-1)

Puerariae Lobatae Radix (1.35 g·kg-1)

and Astragali Radix-Puerariae Lobatae Radix decoction (4.05 g·kg-1) were given respectively in corresponding treatment groups. After 30 days treatment

the rats were sacrificed. The contents of insulin (Ins)

insulin receptor (InsR) and tumor necrosis factor α (TNF-α) in rats liver tissue were detected by enzyme-linked immunosorbent assay (ELISA). Real-time PCR was used to detect adiponectin receptors 1 (AdipoR1)

AMP-activated protein kinase (AMPK)

glucose transporter-4 (GLUT-4)

peroxisome proliferator-activated receptor α (PPARα) and fatty acid transport protein 4 (FATP4)

and p38 mitogen-activated protein kinase (p38 MAPK) mRNA expression in rat liver tissues(P<0.05). Result: Astragali Radix

Puerariae Lobatae Radix and their compatibility increased the level of Ins (except Puerariae Lobatae Radix) and InsR

rised AdipoR1

AMPK(except Puerariae Lobatae Radix)

GLUT-4

PPARα and FATP4 mRNA

lowered TNF-α(except Puerariae Lobatae Radix) and p38 MAPK mRNA expression in liver tissues of diabetes rats.In regulating InsR

PPARα mRNA and TNF-α

Astragali Radix showed better effect than the compatibility of Astragali Radix and Puerariae Lobatae Radix. Conclusion: Astragali Radix-Puerariae Lobatae Radix compatibility presented particular emphasis on lowering glucose-lipid and anti-inflammatory

and the mechanism may be associated with increasing the liver Ins

GLUT-4

FATP4 mRNA and lowering p38 MAPK mRNA levels.

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