加味二妙颗粒剂与传统汤剂指纹图谱及指标成分含量的比较

邹静; 李琳; 任青玲; 刘志辉

doi:10.13422/j.cnki.syfjx.20181102

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加味二妙颗粒剂与传统汤剂指纹图谱及指标成分含量的比较

药剂与炮制 | 更新时间：2024-01-04

- 加味二妙颗粒剂与传统汤剂指纹图谱及指标成分含量的比较
- Comparison of Fingerprints and Index Components Contents of Jiawei Ermiao Traditional Decoction and Granules
- 中国实验方剂学杂志 2018年24卷第12期页码：14-20
- 作者机构：
- 作者简介：
- 基金信息：
  
  江苏省中医院2016年度院级课题（Y16021）;江苏省高校优势学科项目（PATA2014）
- DOI：10.13422/j.cnki.syfjx.20181102
  中图分类号： R286.0
- 纸质出版：2018
- 稿件说明：
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邹静, 李琳, 任青玲, 等. 加味二妙颗粒剂与传统汤剂指纹图谱及指标成分含量的比较[J]. 中国实验方剂学杂志, 2018,24(12):14-20.

ZOU Jing, LI Lin, REN Qing-ling, et al. Comparison of Fingerprints and Index Components Contents of Jiawei Ermiao Traditional Decoction and Granules[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24(12): 14-20.
邹静, 李琳, 任青玲, 等. 加味二妙颗粒剂与传统汤剂指纹图谱及指标成分含量的比较[J]. 中国实验方剂学杂志, 2018,24(12):14-20. DOI： 10.13422/j.cnki.syfjx.20181102.

ZOU Jing, LI Lin, REN Qing-ling, et al. Comparison of Fingerprints and Index Components Contents of Jiawei Ermiao Traditional Decoction and Granules[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24(12): 14-20. DOI： 10.13422/j.cnki.syfjx.20181102.

摘要

目的：比较加味二妙颗粒剂与传统汤剂中7个化学成分的含量，建立该颗粒剂与汤剂的指纹图谱并进行物质基础一致性评价。方法：在前期研究基础上，通过已建立的含量测定与指纹图谱方法对加味二妙颗粒剂与传统汤剂中黄柏碱，盐酸小檗碱，盐酸药根碱，（R，S）-告依春，落新妇苷，橙皮苷和白术内酯Ⅲ的含量进行测定，以盐酸小檗碱为参照峰，利用“中药色谱指纹图谱相似度评价系统”（2012版）对加味二妙颗粒剂与传统汤剂进行相似度分析，采用主成分分析（PCA）对颗粒剂与汤剂的相对峰面积进行差异性分析并进行拆方煎煮，从定性角度考察拆方对加味二妙传统汤剂指纹图谱的影响。结果：加味二妙颗粒剂与传统汤剂中落新妇苷、盐酸药根碱、白术内酯Ⅲ的含量相差不大，且颗粒剂中黄柏碱、盐酸小檗碱、橙皮苷和（R，S）-告依春的含量高于传统汤剂。加味二妙颗粒剂确定了20个共有峰，传统汤剂确定了19个共有峰，颗粒剂与汤剂整体物质基础无明显变化，PCA结果表明加味二妙颗粒剂与传统汤剂的相对峰面积有一定差异，各拆方煎煮色谱图无新化合物产生。结论：建立的指纹图谱体现了加味二妙颗粒剂和传统汤剂的整体物质基础，颗粒剂与汤剂的物质基础基本一致，7个指标成分的含量结合指纹图谱能较为全面地体现加味二妙传统颗粒剂与汤剂的内在质量，可为二者的临床应用提供参考依据。

Abstract

Objective: To compare the contents of seven chemical constituents in Jiawei Ermiao granules and traditional decoction

and to establish their fingerprints and evaluate the consistency of material basis. Method: Based on the established analysis method of determination and fingerprint in previous experiments

the contents of phellodendrine

berberine hydrochloride

jatrorrhizine hydrochloride

S)-goitrin

astilbin

hesperidin

atracylenolide Ⅲ in Jiawei Ermiao granules and traditional decoction were determined.Taking berberine hydrochloride as the reference peak

the similarity of the granules and traditional decoction was carried out by similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2012 edition).The difference of relative peak area of the granules and decoction was analyzed by principal component analysis (PCA)

and the decoction was decocted with decomposed recipes

the influence of decomposed recipes on fingerprint of Jiawei Ermiao decoction from the qualitative evaluation. Result: The contents of astilbin

jatrorrhizine hydrochloride

atracylenolide Ⅲ in the granules and decoction had no significant difference

the contents of phellodendrine

berberine hydrochloride

hesperidin and (R

S)-goitrin in the granules was higher than those of the traditional decoction.Twenty common peaks were indicated in the granules and 19 common peaks were indicated in the traditional decoction.There were no significant changes in the overall material basis between the granules and decoction.PCA results showed that the relative peak area of the traditional decoction was significantly different from that of the granules.There was no new compound in HPLC fingerprints of decoction with decomposed recipes. Conclusion: The established fingerprint reflects the overall material basis of Jiawei Ermiao granules and traditional decoction

the material basis of the granules and decoction is basically the same.The contents of these seven components combined with the fingerprints can reflect the intrinsic quality of the granules and traditional decoction

and provide reference for their clinical application.

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