浏览全部资源
扫码关注微信
重庆三峡学院 生物与食品工程学院,三峡库区道地药材绿色种植与深加工重庆市工程实验室, 重庆 404120
赵顺鑫,在读硕士,从事药用植物栽培与环境微生物研究,E-mail:zsxin165@163.com
杜慧慧,副教授,从事药用植物栽培研究,Tel:023-58102522,E-mail:duhuihui2010@163.com; *
周浓,教授,从事中药炮制与资源研究,Tel:023-58576130,E-mail:erhaizn@126.com
纸质出版日期:2022-08-20,
网络出版日期:2022-06-25,
收稿日期:2022-04-18,
扫 描 看 全 文
赵顺鑫,江春阳,邓乔晟等.解钾菌对滇重楼根际土壤微环境影响[J].中国实验方剂学杂志,2022,28(16):172-179.
ZHAO Shunxin,JIANG Chunyang,DENG Qiaosheng,et al.Effect of Potassium Solubilizing Bacteria on Rhizosphere Soil Microenvironment of Paris polyphylla var. yunnanensis[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(16):172-179.
赵顺鑫,江春阳,邓乔晟等.解钾菌对滇重楼根际土壤微环境影响[J].中国实验方剂学杂志,2022,28(16):172-179. DOI: 10.13422/j.cnki.syfjx.20221017.
ZHAO Shunxin,JIANG Chunyang,DENG Qiaosheng,et al.Effect of Potassium Solubilizing Bacteria on Rhizosphere Soil Microenvironment of Paris polyphylla var. yunnanensis[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(16):172-179. DOI: 10.13422/j.cnki.syfjx.20221017.
目的
2
研究接种解钾菌对滇重楼根际土壤微环境的影响。
方法
2
通过室温盆栽实验,研究接种不同解钾菌对滇重楼根际土壤理化性质,微生物群落结构及土壤酶活性的影响,并进行相关性分析。
结果
2
接种不同解钾菌能显著性影响滇重楼根际土壤理化性质,其中速效氮的质量分数为24.5~90.5 mg·kg
-1
、速效磷质量分数为2.53~25.9 mg·kg
-1
、速效钾质量分数为132~312 mg·kg
-1
。土壤pH为7.08~7.75,均符合滇重楼种植最适范围。接种不同解钾菌可不同程度影响根际土壤中细菌、放线菌和真菌数量,土壤由真菌型向细菌型转化,标志着土壤肥力得到提高。同时也会影响根际土壤酶活性,中性磷酸酶、蛋白酶和多酚氧化酶活性呈现增强的趋势。相关性分析发现,细菌数量与真菌数量呈负相关(
r
=-0.856,
P
<
0.01),与放线菌数量、速效氮、速效钾含量呈正相关,土壤pH呈负相关性。
结论
2
接种解钾菌能有效提高滇重楼根际土壤中速效钾、速效氮、速效磷等养分含量,提高土壤肥力,对滇重楼连作障碍起到了一定的缓解作用,为滇重楼的绿色可持续发展奠定理论基础。
Objective
2
To investigate the effects of the inoculation of potassium-solubilizing bacteria on the rhizosphere soil microenvironment of
Paris polyphylla
var.
yunnanensis
.
Method
2
The effects of different potassium-solubilizing bacteria on the physical and chemical properties, microbial community structure, and soil enzyme activity in the rhizosphere soil of
P. polyphylla
var.
yunnanensis
were investigated by pot planting at room temperature. The correlation of various indexes was analyzed.
Result
2
The inoculation with different potassium-solubilizing bacteria could significantly affect the physical and chemical properties of rhizosphere soil of
P. polyphylla
var.
yunnanensis
. The mass fractions of available nitrogen, available phosphorus, and available potassium were 24.5-90.5 mg·kg
-1
, 2.53-25.9 mg·kg
-1
, and 132-312 mg·kg
-1
, respectively, and the soil pH was 7.08-7.75, which were in line with the optimal ranges of
P. polyphylla
var.
yunnanensis
planting. The inoculation of different potassium-solubilizing bacteria could affect the number of bacteria, actinomycetes, and fungi in rhizosphere soil to varying degrees. The transformation of soil from "fungal type" to "bacterial type" marks the improvement of soil fertility. It also affected the enzyme activity of rhizosphere soil, and the activities of neutral phosphatase, protease, and polyphenol oxidase showed an increasing trend. The correlation analysis showed that the number of bacteria was negatively correlated with the number of fungi (
r
=-0.856,
P
<
0.01), positively correlated with the number of actinomycetes, the content of available nitrogen and available potassium, and negatively correlated with soil pH.
Conclusion
2
The inoculation of potassium-solubilizing bacteria can effectively improve the content of available potassium, available nitrogen, available phosphorus, and other nutrients in the rhizosphere soil of
P. polyphylla
var.
yunnanensis
, improve soil fertility, alleviate the continuous cropping obstacles of
P. polyphylla
var.
yunnanensis
, and lay a theoretical foundation for the green and sustainable development of
P. polyphylla
var.
yunnanensis
.
滇重楼解钾菌根际土壤理化性质微生物群落结构土壤酶
Paris polyphylla var. yunnanensispotassium-solubilizing bacteriarhizosphere soilphysical and chemical propertiesmicrobial community structuresoil enzyme
武珊珊,高文远,段宏泉,等.重楼化学成分和药理作用研究进展[J].中草药,2004,35(3):110-113.
谷付旗,季秀玲,魏云林.土壤硅酸盐细菌的研究进展[J].中国微生态学杂志,2013,25(5):609-611,616.
张路.杨树新杂种无性系抗溃疡病研究[D].杨凌:西北农林科技大学,2018.
於忠祥.基于土壤学理论的土地整理研究[J].安徽农业大学学报,2007,34(1):129-133.
龚雪蛟,秦琳,刘飞,等.有机类肥料对土壤养分含量的影响[J].应用生态学报,2020,31(4):1403-1416.
荆佳强,萨仁其力莫格,秦洁,等.利用方式对贝加尔针茅草原土壤微生物群落结构与土壤酶活性的影响[J].中国草地学报,2022,44(2):33-40.
曾成城,陈锦平,魏虹,等.水淹生境下秋华柳对Cd污染土壤微生物数量及酶活性的影响[J].生态学报,2017,37(13):4327-4334.
陈智,于贵瑞.土壤微生物碳素利用效率研究进展[J].生态学报,2020,40(3):756-767.
石子为,杨少华,张丽霞,等.基于地形土壤因子的滇重楼生态适宜性区划研究[J].西南农业学报,2017,30(8):1904-1909.
王骞,邬佩宏,杨敏,等.滇重楼根茎品质与根际土壤因子的相关性分析[J].中草药,2020,51(22):5839-5849.
周浓,朱芙蓉,杜慧慧,等.不同生境滇重楼根际解钾菌的筛选与鉴定[J].中国中药杂志,2021,46(5):1073-1078.
鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000:14-24.
易海艳,马刘峰,林宁,等.新疆叶尔羌河流域棉田土壤养分分析与评价[J].江苏农业科学,2015(7):393-396.
高冬梅.环境微生物实验[M].青岛:中国海洋大学出版社,2014:32-39.
关松荫.土壤酶及其研究方法[M].北京:农业出版社,1986:260-360.
杨兰芳,曾巧,李海波,等.紫外分光光度法测定土壤过氧化氢酶活性[J].土壤通报,2011,42(1):207-210.
郝建朝,吴沿友,连宾,等.土壤多酚氧化酶性质研究及意义[J].土壤通报,2006,37(3):470-474.
毛玉东,梁社往,何忠俊,等.土壤pH对滇重楼生长、养分含量和总皂甙含量的影响[J].西南农业学报,2011,24(3):985-989.
董悦,张永清,刘彩彩.晋中市土壤养分空间分布与影响因子的相关性[J].生态学报,2018,38(23):8621-8629.
佟玉洁.自制环保酵素改善土壤肥力试验研究[J].安徽农业科学,2017,45(26):119-121.
杨冬艳,高晶霞,桑婷,等.溶磷菌和解钾菌对拱棚连作辣椒生长及土壤养分含量的影响[J].北方园艺,2019(6):1-6.
张静,马玲,丁新华,等.扎龙湿地不同生境土壤微生物生物量碳氮的季节变化[J].生态学报,2014,34(13):3712-3719.
乔蓬蕾,周新刚,吴凤芝.不同连作茬次黄瓜根际土壤微生物群落的T-RFLP分析[J].生态学杂志,2014,33(10):2640-2649.
李正洲,丁绪,赖茅田,等.外施木霉对甜瓜土壤酶活性的影响[J].黑龙江农业科学,2021(12):53-57.
李星月,易军,符慧娟,等.光合细菌和生物包衣对土壤酶活与油菜产量的影响[J].中国农学通报,2022,38(2):87-91.
尚海丽,毕银丽,李少朋,等.解钾细菌对不同黏土矿物含量土壤微生物和酶活性的影响[J].安徽农业科学,2018,46(31):124-129.
0
浏览量
11
下载量
4
CSCD
关联资源
相关文章
相关作者
相关机构