芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4，NF-<italic style="font-style: italic">κ</italic>B p65和IL-6表达的调控作用

徐敏; 王凤仪; 赵党生; 蒲晓薇; 张磊; 张小元; 李琳; 李淑霞; 李志平

doi:10.13422/j.cnki.syfjx.20201403

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芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4，NF-κB p65和IL-6表达的调控作用

经典名方 | 更新时间：2020-09-09

- 芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4，NF-κB p65和IL-6表达的调控作用
- Effect of Shaoyaotang on Expressions of TLR4, NF-κB p65 and IL-6 in Rats with Damp-heat Ulcerative Colitis
- 中国实验方剂学杂志 2020年26卷第14期页码：53-58
- 作者机构：
  
  甘肃中医药大学，兰州 730000
- 作者简介：
  
  徐敏，在读硕士，从事四诊理论研究与分析，E-mail：289780966@qq.com
  王凤仪，博士，硕士生导师，教授，从事中医四诊理论研究与分析，E-mail：wfy@gszy.edu.cn
- 基金信息：
  
  国家自然科学基金项目(81560746)
- DOI：10.13422/j.cnki.syfjx.20201403
  中图分类号： R2-0;R22;R285.5;R289
- 纸质出版日期：2020-07-20，
  
  网络出版日期：2020-04-27，
- 稿件说明：
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徐敏,王凤仪,赵党生等.芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4，NF-κB p65和IL-6表达的调控作用[J].中国实验方剂学杂志,2020,26(14):53-58.

XU Min,WANG Feng-yi,ZHAO Dang-sheng,et al.Effect of Shaoyaotang on Expressions of TLR4, NF-,κ,B p65 and IL-6 in Rats with Damp-heat Ulcerative Colitis[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):53-58.
徐敏,王凤仪,赵党生等.芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4，NF-κB p65和IL-6表达的调控作用[J].中国实验方剂学杂志,2020,26(14):53-58. DOI： 10.13422/j.cnki.syfjx.20201403.

XU Min,WANG Feng-yi,ZHAO Dang-sheng,et al.Effect of Shaoyaotang on Expressions of TLR4, NF-,κ,B p65 and IL-6 in Rats with Damp-heat Ulcerative Colitis[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):53-58. DOI： 10.13422/j.cnki.syfjx.20201403.

摘要

目的

以Toll样受体4(TLR4)/核转录因子-

B(NF-

B)信号通路为基础，探讨芍药汤治疗湿热内蕴型溃疡性结肠炎(UC)的作用机制。

方法

取50只Wistar大鼠，雌雄各半，采用病证结合方法，以高脂高糖辛辣食物及免疫复合法，联合2，4，6-三硝基苯磺酸（TNBS）结合乙醇复合法复制湿热内蕴型UC大鼠模型。造模成功后，将模型大鼠随机分为模型组、柳氮磺吡啶组及芍药汤低、中、高剂量组，另取10只大鼠（雌雄各半）作为正常组。芍药汤低、中、高剂量组按6，12，24 g·kg

-1

灌胃，柳氮磺吡啶组按1 g·kg

-1

剂量灌胃，正常组给予等体积生理盐水，连续21 d。末次给药后采集结肠样本，苏木素-伊红(HE)染色观察结肠组织病理变化，运用实时荧光定量聚合酶链式反应（Real-time PCR）检测结肠组织中TLR4，NF-

B p65和IL-6 mRNA的表达，蛋白免疫印迹法（Western blot）检测结肠组织中TLR4，NF-

B p65和IL-6蛋白的表达。

结果

与正常组比较，模型组结肠组织损坏较明显，模型组TLR4，NF-

B p65和IL-6 mRNA及蛋白相对表达量明显升高(

0.05)；与模型组比较，柳氮磺吡啶组、芍药汤各剂量组结肠组织损坏明显改善，TLR4，NF-

B p65和IL-6 mRNA及蛋白表达量明显下降(

0.05)，芍药汤各剂量组中以芍药汤高剂量组最为明显。

结论

芍药汤可通过调控TLR4/NF-

B通路中TLR4，NF-

B p65和IL-6 mRNA及蛋白的表达，抑制UC病情的发展。

Abstract

Objective

To explore the mechanism of Shaoyaotang in the treatment of ulcerative colitis (UC) based on toll-like receptor 4 (TLR4)/nuclear factor kappaB (NF-

B) signaling pathway.

Method

A total of 50 Wistar rats were selected

including half male and half female. The damp-heat UC rat model was replicated by the methods of the combination of diseases and syndromes and the combination of 2

6-nitrobenzene sulfonic acid (TNBS) and ethanol. After the successful modeling

the model rats were randomly divided into model group

salazulesulfonate group

and low

medium and high-dose Shaoyaotang groups

and 10 rats (half male and half female) were selected as the blank control group. Low

medium and high-dose Shaoyaotang groups were given 6

24 g·kg

-1

gavage

and salazonyl arsenic group was given 1 g·kg

-1

gavage. Blank control group was given the equal volume of normal saline for 21 consecutive days. Colon samples were collected after the last administration

and the expressions of TLR4

NF-

B p65 and IL-6 mRNA in colon tissues were detected by fluorescent quantitative polymerase chain reaction (Real-time PCR）

and the expressions of TLR4

NF-

B p65 and IL-6 protein in colon tissues were detected by Western blot.

Result

Compared with the blank control group

the relative expressions of TLR4

NF-

B p65

IL-6 mRNA and protein in the model group were significantly increased (

0.05). Compared with the model group

the expression levels of TLR4

NF-

B p65 and IL-6 mRNA and protein in the salazopyridine group and Shaoyaotang groups were significantly decreased (

0.05).

Conclusion

Shaoyaotang can inhibit the development of UC by regulating the expressions of TLR4

NF-

B p65 and IL-6 mRNA and proteins in the TLR4/NF-

B pathway.

关键词

芍药汤溃疡性结肠炎Toll样受体4（TLR4）核转录因子-κB p65（NF-κB p65）白细胞介素-6（IL-6）

Keywords

Shaoyaotangulcerative colitisToll-like receptor 4 (TLR4)nuclear factor-kappaB p65 (NF-κB p65)interleukin 6(IL-6)

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Effect of Shaoyaotang on Expressions of TLR4, NF-κB p65 and IL-6 in Rats with Damp-heat Ulcerative Colitis

DOI：10.13422/j.cnki.syfjx.20201403

摘要

Abstract

关键词

Keywords

references