芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4，NF-<italic style="font-style: italic">κ</italic>B p65和IL-6表达的调控作用

徐敏; 王凤仪; 赵党生; 蒲晓薇; 张磊; 张小元; 李琳; 李淑霞; 李志平

doi:10.13422/j.cnki.syfjx.20201403

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芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4，NF-κB p65和IL-6表达的调控作用

经典名方 | 更新时间：2020-09-09

- 芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4，NF-κB p65和IL-6表达的调控作用
- Effect of Shaoyaotang on Expressions of TLR4, NF-κB p65 and IL-6 in Rats with Damp-heat Ulcerative Colitis
- 中国实验方剂学杂志 2020年26卷第14期页码：53-58
- 作者机构：
  
  甘肃中医药大学，兰州 730000
- 作者简介：
  
  徐敏，在读硕士，从事四诊理论研究与分析，E-mail：289780966@qq.com
  王凤仪，博士，硕士生导师，教授，从事中医四诊理论研究与分析，E-mail：wfy@gszy.edu.cn
- 基金信息：
  
  国家自然科学基金项目(81560746)
- DOI：10.13422/j.cnki.syfjx.20201403
  中图分类号： R2-0;R22;R285.5;R289
- 网络出版日期：2020-04-27，
  
  纸质出版日期：2020-07-20
- 稿件说明：
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徐敏,王凤仪,赵党生等.芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4，NF-κB p65和IL-6表达的调控作用[J].中国实验方剂学杂志,2020,26(14):53-58.

XU Min,WANG Feng-yi,ZHAO Dang-sheng,et al.Effect of Shaoyaotang on Expressions of TLR4, NF-,κ,B p65 and IL-6 in Rats with Damp-heat Ulcerative Colitis[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):53-58.
徐敏,王凤仪,赵党生等.芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4，NF-κB p65和IL-6表达的调控作用[J].中国实验方剂学杂志,2020,26(14):53-58. DOI： 10.13422/j.cnki.syfjx.20201403.

XU Min,WANG Feng-yi,ZHAO Dang-sheng,et al.Effect of Shaoyaotang on Expressions of TLR4, NF-,κ,B p65 and IL-6 in Rats with Damp-heat Ulcerative Colitis[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):53-58. DOI： 10.13422/j.cnki.syfjx.20201403.

摘要

目的

以Toll样受体4(TLR4)/核转录因子-

B(NF-

B)信号通路为基础，探讨芍药汤治疗湿热内蕴型溃疡性结肠炎(UC)的作用机制。

方法

取50只Wistar大鼠，雌雄各半，采用病证结合方法，以高脂高糖辛辣食物及免疫复合法，联合2，4，6-三硝基苯磺酸（TNBS）结合乙醇复合法复制湿热内蕴型UC大鼠模型。造模成功后，将模型大鼠随机分为模型组、柳氮磺吡啶组及芍药汤低、中、高剂量组，另取10只大鼠（雌雄各半）作为正常组。芍药汤低、中、高剂量组按6，12，24 g·kg

-1

灌胃，柳氮磺吡啶组按1 g·kg

-1

剂量灌胃，正常组给予等体积生理盐水，连续21 d。末次给药后采集结肠样本，苏木素-伊红(HE)染色观察结肠组织病理变化，运用实时荧光定量聚合酶链式反应（Real-time PCR）检测结肠组织中TLR4，NF-

B p65和IL-6 mRNA的表达，蛋白免疫印迹法（Western blot）检测结肠组织中TLR4，NF-

B p65和IL-6蛋白的表达。

结果

与正常组比较，模型组结肠组织损坏较明显，模型组TLR4，NF-

B p65和IL-6 mRNA及蛋白相对表达量明显升高(

0.05)；与模型组比较，柳氮磺吡啶组、芍药汤各剂量组结肠组织损坏明显改善，TLR4，NF-

B p65和IL-6 mRNA及蛋白表达量明显下降(

0.05)，芍药汤各剂量组中以芍药汤高剂量组最为明显。

结论

芍药汤可通过调控TLR4/NF-

B通路中TLR4，NF-

B p65和IL-6 mRNA及蛋白的表达，抑制UC病情的发展。

Abstract

Objective

To explore the mechanism of Shaoyaotang in the treatment of ulcerative colitis (UC) based on toll-like receptor 4 (TLR4)/nuclear factor kappaB (NF-

B) signaling pathway.

Method

A total of 50 Wistar rats were selected

including half male and half female. The damp-heat UC rat model was replicated by the methods of the combination of diseases and syndromes and the combination of 2

6-nitrobenzene sulfonic acid (TNBS) and ethanol. After the successful modeling

the model rats were randomly divided into model group

salazulesulfonate group

and low

medium and high-dose Shaoyaotang groups

and 10 rats (half male and half female) were selected as the blank control group. Low

medium and high-dose Shaoyaotang groups were given 6

24 g·kg

-1

gavage

and salazonyl arsenic group was given 1 g·kg

-1

gavage. Blank control group was given the equal volume of normal saline for 21 consecutive days. Colon samples were collected after the last administration

and the expressions of TLR4

NF-

B p65 and IL-6 mRNA in colon tissues were detected by fluorescent quantitative polymerase chain reaction (Real-time PCR）

and the expressions of TLR4

NF-

B p65 and IL-6 protein in colon tissues were detected by Western blot.

Result

Compared with the blank control group

the relative expressions of TLR4

NF-

B p65

IL-6 mRNA and protein in the model group were significantly increased (

0.05). Compared with the model group

the expression levels of TLR4

NF-

B p65 and IL-6 mRNA and protein in the salazopyridine group and Shaoyaotang groups were significantly decreased (

0.05).

Conclusion

Shaoyaotang can inhibit the development of UC by regulating the expressions of TLR4

NF-

B p65 and IL-6 mRNA and proteins in the TLR4/NF-

B pathway.

关键词

Keywords

references

MAVROUDIS G , MAGNUSSON M K , ISAKSSON S , et al . Mucosal and systemic immune profiles differ during early and late phase of the disease in patients with active ulcerative colitis [J]. J Crohns Colitis , 2019 , 13 ( 11 ): 1450 - 1458 .

MI H , ZHONG L , HUANG T , et al . Chinese herbal medicinefor ulcerative colitis:a systematic review protocol [J]. World J Tradit Chin Med , 2017 , 3 ( 2 ): 16 - 19 .

TUN X , YASUKAWA K , YAMADA K I , Nitric oxide is involved in activation of Toll-like receptor 4 signaling through tyrosine nitration of src homology protein tyrosine phosphatase 2 in murine dextran sulfate-induced colitis [J]. Biol Pharm Bull , 2018 , 41 : 1843 - 1852 .

XUE B , LIU X L , DONG W W , et al . EGCG maintains Th1/Th2 balance and mitigates ulcerative colitis induced by dextran sulfate sodium through TLR4/MyD88/NF- κ B signaling pathway in rats [J]. Can J Gastroenterol Hepatol , 2017 , doi: 10.1155/2017/3057268 http://dx.doi.org/10.1155/2017/3057268 .

冀茂昌 . 中医分期治疗溃疡性结肠炎临床研究 [J]. 河南中医 , 2016 , 36 ( 12 ): 2182 - 2185 .

李毅 , 刘艳 , 刘力 . 溃疡性结肠炎的中医辨证分型统计分析 [J]. 中医药导报 , 2016 , 22 ( 11 ): 94 - 95,98 .

王移飞 , 王凤仪 , 徐兰萍 , 等 . 芍药汤经HMGB1调节湿热型溃疡性结肠炎大鼠MyD88和NF- κ B的分子机制 [J]. 中国实验方剂学杂志 , 2018 , 24 ( 12 ): 86 - 91 .

王凤仪 , 赵党生 , 蒲晓薇 , 等 . 芍药汤对湿热内蕴型溃疡性结肠炎大鼠模型结肠组织中AP-1,TNF- α 表达的影响 [J]. 中国实验方剂学杂志 , 2019 , 25 ( 16 ): 7 - 11 .

翁一洁 , 郑学宝 . 湿热型溃疡性结肠炎大鼠模型的建立与研究 [J]. 时珍国医国药 , 2011 , 22 ( 10 ): 2522 - 2525 .

赵文畅 , 刘羽丹 , 沙磊 . 溃疡性结肠炎的免疫机制研究进展 [J]. 医学综述 , 2018 , 24 ( 22 ): 4421 - 4426,4432 .

YAO D B , DONG M , DAI C L , et al . Inflammation and inflammatory cytokine contribute to the initiation and development of ulcerative colitis and its associated cancer [J]. Inflamm Bowel Dis , 2019 , 25 : 1595 - 1602 .

赵保胜 , 霍海如 , 姜廷良 . Toll样受体4的研究及现状 [J]. 中国临床药理学与治疗学 , 2007 , 12 ( 1 ): 19 - 22 .

RASHIDIAN A , MUHAMMADNEJAD A , DEHPOUR A R , et al . Atorvastatin attenuates TNBS-induced rat colitis: the involvement of the TLR4/NF- κ B signaling pathway [J]. Inflammopharmacology , 2016 , 24 : 109 - 118 .

秦幸茹 , 赵相轩 , 闫浩 , 等 . 溃疡性结肠炎相关分子机制研究进展 [J]. 中国临床研究 , 2018 , 31 ( 4 ): 558 - 561 .

BAKER R G , HAYDEN M S , GHOSH S . NF- κ B,inflammation, and metabolic disease [J]. Cell Metab , 2011 , 13 : 11 - 22 .

SARTOR R B . Mechanisms of disease:pathogenesis of Crohn's disease and ulcerative colitis [J]. Nat Clin Pract Gastroenterol Hepatol , 2006 , 3 ( 7 ): 390 - 407 .

黄循铷 , 王承党 , 王瑞幸 , 等 . 溃疡性结肠炎小鼠肠道通透性改变与TNF- α 及NF- κ B p65的关系 [J]. 中国应用生理学杂志 , 2016 , 32 ( 2 ): 112 - 115,193 .

WINE E , MACK D R , HYAMS J , et al . Interleukin-6 is associated with steroid resistance and reflects disease activity in severe pediatric ulcerative colitis [J]. J Crohns Colitis , 2013 , 7 : 916 - 22 .

ZHENG W , XUE W , WANG C L , et al . Tryptanthrin protects mice against dextran sulfate sodium-induced colitis through inhibition of TNF- α /NF- κ B and IL-6/STAT3 pathways [J]. Molecules , 2018 , 23 ( 5 ): 1062 .

KIM K Y , OH T W , DO H J , et al . Acer palmatum thumb.Ethanol extract alleviates interleukin-6-induced barrier dysfunction and dextran sodium sulfate-induced colitis by improving intestinal barrier function and reducing inflammation [J]. J Immunol Res , 2018 , doi: 10.1155/2018/5718396 http://dx.doi.org/10.1155/2018/5718396 .

GRIVENNIKOV S , KARIN E , TERZIC J , et al . IL-6 and Stat3 are required for survival of intestinal epithelial cells and development of colitis-associated cancer [J]. Cancer Cell , 2009 , 15 : 103 - 113 .

LEE M J , LEE J K , CHOI J W , et al . Interleukin-6 induces S100A9 expression in colonic epithelial cells through STAT3 activation in experimental ulcerative colitis [J]. PLoS One , 2012 , 7 ( 9 ): e38801 .

沈洪 , 邢敬 . 中西医结合治疗溃疡性结肠炎的优势及临床应用 [J]. 医学研究生学报 , 2019 , 32 ( 6 ): 586 - 590 .

钟宇 , 郑学宝 , 叶华 , 等 . 芍药汤对溃疡性结肠炎大鼠TLR4/NF- κ B通路的影响 [J]. 中国中药杂志 , 2019 , 44 ( 7 ): 1450 - 1456 .

刘芳 , 雷娜 , 唐学贵 . 当归芍药散合槐花散加减治疗溃疡性结肠炎活动期大肠湿热证的临床观察 [J]. 中国实验方剂学杂志 , 2019 , 25 ( 20 ): 82 - 87 .

戴路明 , 朱磊 , 沈洪 . 基于 β 2AR / β -arrestin2/NF- κ B信号通路的清肠化湿颗粒防治溃疡性结肠炎的作用机制 [J]. 中国实验方剂学杂志 , 2018 , 24 ( 9 ): 86 - 94 .

夏修文 , 陈桥桥 , 丁斗 , 等 . 芍药汤治疗溃疡性结肠炎的作用机制研究进展 [J]. 成都中医药大学学报 , 2018 , 41 ( 3 ): 119 - 123 .

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Effect of Shaoyaotang on Expressions of TLR4, NF-κB p65 and IL-6 in Rats with Damp-heat Ulcerative Colitis

DOI：10.13422/j.cnki.syfjx.20201403

摘要

Abstract

关键词

Keywords

references