Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats

Cong ZHANG; Jian-xun LIU; Ming-qian SUN; Ye-hao ZHANG; Chang-ying REN; Chun-yuan YIN

doi:10.13422/j.cnki.syfjx.20191552

您当前的位置：

首页 >

文章列表页 >

Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats

Drug Metabolism | 更新时间：2021-02-09

- Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 14, Pages: 136-141(2019)
- 作者机构：
  
  1.中国中医科学院　西苑医院　基础医学研究所，中药药理北京市重点实验室，北京　 100091；
  2.北京中医药大学，北京　 100029
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191552
  CLC： R22; R969.1; R743; O657; R28; C37
- Published：20 July 2019，
  
  Published Online：22 April 2019，
  
  Received：10 January 2019，
- 稿件说明：
扫描看全文
Cong ZHANG, Jian-xun LIU, Ming-qian SUN, et al. Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):136-141(2019)
DOI：

Cong ZHANG, Jian-xun LIU, Ming-qian SUN, et al. Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):136-141(2019) DOI： 10.13422/j.cnki.syfjx.20191552.

摘要

目的：

从代谢组学角度研究塞络通胶囊治疗急性多发性脑梗死大鼠模型的作用机制。

方法：

24只SD大鼠随机分为假手术组、模型组和塞络通组(33 mg·kg

－1

)，通过大鼠颈内动脉注射荧光微球的方法建立急性多发性脑梗死大鼠模型。手术成功后，塞络通组大鼠通过十二指肠注射给药，给药体积2 mL·kg

－1

。基于UPLC-Q-TOF-MS分析各组大鼠脑组织中内源性代谢物，应用主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA)进行模式识别及生物标志物的鉴定。

结果：

通过模式识别发现假手术组与模型组的代谢轮廓有明显差异，并鉴定了10个与急性脑缺血相关的生物标志物。与假手术组相比，模型组中

-乙酰天门冬氨酸(NAA)，延胡索酸，谷胱甘肽，脱氢抗坏血酸，天门冬氨酸以及

-腺苷同型半胱氨酸含量下降，精氨酸、瓜氨酸、酵母氨酸、乙内酰脲-5-丙酸含量升高；而塞络通胶囊则可使上述10个异常变化的生物标志物水平显著回调。

结论：

塞络通胶囊主要调节的代谢途径有NAA代谢、精氨酸代谢、能量代谢及氧化应激等。

Abstract

Objective:

To explore the mechanism of Sailuotong capsules in treating acute cerebral ischemia from the perspective of metabonomics.

Method:

A total of 24 SD rats were randomly divided into 3 groups

including sham-operated group

model group and Sailuotong group (33 mg·kg

-1

). The rat model of acute multiple cerebral infarction was established by injecting fluorescent microspheres into internal carotid artery. After the successful operation

rats in Sailuotong group were administered by duodenal injection immediately

and the dosage volume was 2 mL·kg

-1

. Endogenous metabolites in rat brain tissues of each group were determined by UPLC-Q-TOF-MS. The relevant data and biomarkers were analyzed by principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA).

Result:

The analysis of pattern recognition indicated that the metabolite profiles in model group and sham-operated group were separated obviously

and ten biomarkers related to acute cerebral ischemia were also identified. Compared with the sham-operated group

contents of

-acetylaspartate (NAA)

fumaric acid

glutathione

dehydroascorbic acid

aspartic acid and

-adenosylhomocysteine were decreased

while the contents of arginine

citrulline

saccharopine and hydantoin-5-propionic acid were increased in the model group. Meanwhile

the ten abnormal biomarkers mentioned above got restoration in Sailuotong group.

Conclusion:

The main regulated metabolic pathways of Sailuotong capsules are NAA metabolism

arginine metabolism

energy metabolism

oxidative stress

etc.

关键词

塞络通胶囊急性脑缺血代谢组学生物标志物代谢途径N-乙酰天门冬氨酸代谢精氨酸代谢

Keywords

Sailuotong capsulesacute cerebral ischemiametabonomicsbiomarkersmetabolic pathwaysN-acetylaspartate metabolismarginine metabolism

references

YIN Y W, LI J C, LI B H, et al. Toll-like receptor 4 gene Asp299Gly polymorphism in ischemic cerebrovascular disease: a meta-analysis [J].Int J Neurosci, 2014, 124(4): 252-260.

张静，赵建军，杨伟，等．基于中医证候特征的缺血性中风复发风险的横断面分析[J]．中国实验方剂学杂志，2018，24(12)：194-198．

WANG H P, SHI S J, YAN W J, et al. Ischemic stroke susceptibility gene in a Northern Han Chinese population [J].Neural Regen Res, 2013, 8(20): 1881-1891.

吴玉芙，刘晓红，郭伟成，等．补阳还五汤对气虚血瘀型脑梗死患者脑血管储备功能的影响[J]．中国实验方剂学杂志，2017，23(12)：162-167．

张颖，林力，刘建勋，等．塞络通灌胃后人参皂苷在大鼠体内的药代动力学及脑分布研究[J]．中国中药杂志，2014，39(2)：316-321．

张颖，林力，刘建勋，等．中药复方塞络通中银杏内酯类成分的药代动力学及脑分布研究[J]．世界科学技术—中医药现代化，2014，16(7)：1458-1464．

徐立，宋文婷，林成仁，等．塞络通胶囊对大鼠多发性脑梗死恢复期Glu和GABA合成以及NMDA受体亚型表达的影响[J]．药学学报，2012，47(7)：870-877．

徐立，丛伟红，魏翠娥，等．维脑康对小鼠记忆障碍的影响[J]．中药药理与临床，2007，23(6)：60-62．

徐立，林成仁，刘建勋，等．塞络通胶囊对多发梗死性痴呆大鼠认知功能障碍的干预作用[J]．中国中药杂志，2012，37(19)：2943-2946．

徐立，刘建勋，丛伟红，等．维脑康胶囊对慢性脑低灌注大鼠脑内胆碱能系统及清除自由基能力的影响[J]．中国中药杂志，2008，33(5)：531-534．

任建勋，徐立，林成仁，等．塞络通胶囊在络脉瘀阻证脑梗死大鼠缺血时间窗外给药的保护作用[J]．中华中医药杂志，2012，27(12)：3060-3063．

郭慧，崔扬，王秋红，等．基于代谢组学技术的中药复方研究近况[J]．中国实验方剂学杂志，2017，23(1)：213-219．

孟胜喜，霍清萍，王兵，等．代谢组学技术在中医药防治缺血性脑血管病研究中的应用[J]．中医杂志，2016，57(17)：1515-1518，1521．

Takagi N, Miyake K, Taguchi T, et al. Failure in learning task and loss of cortical cholinergic fibers in microsphere-embolized rats [J].Exp Brain Res, 1997, 114(2): 279-287.

Koller K J, Coyle J T. Ontogenesis of N-acetyl-aspartate and N-acetyl-aspartyl-glutamate in rat brain [J].Brain Res, 1984, 317(1): 137-140.

蔺俊斌，廖维靖．N-乙酰天冬氨酸与缺血性卒中的研究进展[J]．中国脑血管病杂志，2011，8(9)：501-504．

刘雪辉，曹平，钟文，等．天智颗粒联合盐酸多奈哌齐片对轻中度肝阳上亢证血管性痴呆的临床分析[J]．中国实验方剂学杂志，2016，22(7)：177-181．

Muñoz Maniega S, Cvoro V, Chappell F M, et al. Changes in NAA and lactate following ischemic stoke: a serial MR spectroscopic imaging study [J].Neurology, 2008, 71(24): 1993-1999.

Demougeot C, Marie C, Giroud M, et al. N-acetylaspartate: a literature review of animal research on brain ischaemia [J].J Neurochem, 2004, 90(4): 776-783.

LEI H, Berthet C, Hirt L, et al. Evolution of the neurochemical profile after transient focal cerebral ischemia in the mouse brain [J].J Cereb Blood Flow Metab, 2009, 29(4): 811-819.

Lemesle M, Walker P, Guy F, et al. Multi-variate analysis predicts clinical outcome 30 days after middle cerebral artery infarction [J].Acta Neurol Scand, 2000, 102(1): 11-17.

van Zandvoort M J, van der Grond J, Kappelle L J, et al. Cognitive deficits and changes in neurometabolites after a lacunar infarct [J].J Neurol, 2005, 252(2): 183-190.

Morris D, Khurasany M, Nguyen T, et al. Glutathione and infection [J].Biochim Biophy Acta, 2013, 1830(5): 3329-3349.

Lyons J, Rauh-Pfeiffer A, YU Y M, et al. Blood glutathione synthesis rates in healthy adults receiving a sulfur amino acid-free diet [J].Proc Natl Acad Sci USA, 2000, 97(10): 5071-5076.

易文龙．谷胱甘肽对新生大鼠脑缺氧缺血脑细胞内钙和脂质过氧化影响的研究[J]．数理医药学杂志，2004，17(4)：371-372．

郭志丽，朱妍，肖红斌，等．与脑梗死关联的氨基酸代谢通路研究进展与发现[J]．中国医药导报，2013，10(26)：24-27．

陈瑞群，廖成彬，郭茜，等．结合血清代谢组学与支持向量回归机揭示右归丸调节肾阳虚证的代谢标志物群及其作用机制[J]．中国实验方剂学杂志，2018，24(13)：62-70．

Rector T S, Bank A J, Mullen K A, et al. Randomized, double-blind, placebo-controlled study of supplemental oral L-arginine in patients with heart failure [J].Circulation, 1996, 93(12): 2135-2141.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Serum Metabonomics Analysis of Colitis Associated Cancer Mice Intervened by Hedyotis diffusa Based on UPLC-Q-TOF-MS

Comparative Analysis of Effect of Houpu Wenzhongtang Combined Decoction and Simple Decoction on Urine Metabonomics of Rats with Deficiency-cold of Spleen and Stomach

Effect of Scrophulariae Radix on Rat Model with Dampness Stagnancy due to Spleen Deficiency and Analysis of Its Liver Metabonomics

Investigation of Intervention of Epimedii Folium Water Extract on Metabolic Profiling Targets by Metabonomics

Application of Metabonomics Method to Identify Possible Effect of Polysaccharide on AD

Related Author

Shu WANG

Xin WANG

Ping SUN

Ying MENG

Guang-shang CAO

Peng ZHOU

Pei-min YANG

Jun YAN

Related Institution

Affiliated Hospital of Shandong University of TCM，Ji'nan

Shandong University of Traditional Chinese Medicine（TCM），Ji'nan

Key Laboratory of Modern Preparation of Traditional Chinese Medicine (TCM),Ministry of Education,Jiangxi University of TCM

Dongdajie Community Health Service Center in Anyuan District

National Resource Center for Chinese Materia Medica，State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs，China Academy of Chinese Medical Sciences

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰