栀子皮、仁、须中3个成分含量测定及指纹图谱

梁献葵; 王艳慧; 雷敬卫; 龚海燕; 唐维维; 谢彩侠; 杨春静

doi:10.13422/j.cnki.syfjx.20190213

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栀子皮、仁、须中3个成分含量测定及指纹图谱

资源与质量评价 | 更新时间：2021-02-09

- 栀子皮、仁、须中3个成分含量测定及指纹图谱
- Determination of Content of Index Components and HPLC Fingerprint of Gardenia jasminoides Pericarp,Seeds and Whiskers
- 中国实验方剂学杂志 2019年25卷第4期页码：193-200
- 作者机构：
  
  1.河南中医药大学，郑州　 450046；
  2.仲景宛西制药股份有限公司，郑州　 450046；
  3.河南中医药大学　第三附属医院，郑州　 450003
- 作者简介：
  
  梁献葵，在读硕士，从事中药质量分析研究，E-mail：646909449@qq.com
  *雷敬卫，博士，硕士生导师，从事中药质量分析研究，Tel：0371-656955281，E-mail：yjs2017tg@126.com
- 基金信息：
  
  河南省中药材开发工程技术研究中心项目(142102313109);河南省科技科技攻关项目(162102310448)
- DOI：10.13422/j.cnki.syfjx.20190213
  中图分类号： R284.1;R282.5;R289;R22
- 纸质出版日期：2019-02-20，
  
  网络出版日期：2018-11-02，
  
  收稿日期：2018-04-12，
- 稿件说明：
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梁献葵, 王艳慧, 雷敬卫, 等. 栀子皮、仁、须中3个成分含量测定及指纹图谱[J]. 中国实验方剂学杂志, 2019,25(4):193-200.

Xian-kui LIANG, Yan-hui WANG, Jing-wei LEI, et al. Determination of Content of Index Components and HPLC Fingerprint of Gardenia jasminoides Pericarp,Seeds and Whiskers[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019,25(4):193-200.
梁献葵, 王艳慧, 雷敬卫, 等. 栀子皮、仁、须中3个成分含量测定及指纹图谱[J]. 中国实验方剂学杂志, 2019,25(4):193-200. DOI： 10.13422/j.cnki.syfjx.20190213.

Xian-kui LIANG, Yan-hui WANG, Jing-wei LEI, et al. Determination of Content of Index Components and HPLC Fingerprint of Gardenia jasminoides Pericarp,Seeds and Whiskers[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019,25(4):193-200. DOI： 10.13422/j.cnki.syfjx.20190213.

摘要

目的：

测定栀子不同部位(皮、仁、须)指标性成分含量及对其指纹图谱进行研究，比较栀子不同部位成分含量及组成差异，为栀子皮、仁药效差异提供理论依据；探究栀子须的开发利用价值，避免栀子药用资源浪费。

方法：

栀子苷、西红花苷Ⅰ 含量测定采用HPLC法；总环烯迷萜苷含量测定采用紫外分光光度法，对栀子不同部位3个成分含量进行分析。采集栀子不同部位HPLC指纹图谱，分别建立不同产地不同加工方法栀子不同部位HPLC指纹图谱共有模式，并采用相似度评价系统软件进行数据分析；对栀子不同部位指纹图谱进行比对分析。

结果：

河南、福建、江西栀子皮、仁指标性成分的含量变化相同，栀子苷含量为福建>河南>江西，福建栀子皮中3种成分含量远远高于河南、江西。西红花苷Ⅰ和总环烯迷萜苷含量为福建>江西>河南，福建、江西总环烯迷萜苷含量远远高于河南。不同产地的栀子须中3个指标性成分含量比较，栀子苷、西红花苷I含量为福建>江西、河南；总环烯迷萜苷含量为福建>江西>河南。同一部位，栀子皮指纹图谱共标定22个共有峰，除了S13～S15外，其他样品的相似度在0.9以上；栀子仁指纹图谱共标定22个共有峰，除了S22～S30外，其他样品的相似度在0.9以上；栀子须指纹图谱共标定16个共有峰，除了S7～S9外，其他样品的相似度在0.9以上。不同部位，栀子须与皮、仁指纹图谱差别较大，须部位峰数减少，峰2，3，5的峰高须>皮>仁；仁部无峰X，峰高须>皮；除峰17外，仁部位图谱各峰的峰高均高于皮部位。

结论：

栀子皮、仁部位指标性成分含量存在显著差异；栀子须中总苷含量较高，可用于提取总环烯醚萜苷。所建指纹图谱可以反映栀子不同部位成分含量差异及种类分布，为栀子的药效物质基础研究提供理论支撑，为临床上栀皮、栀仁分开使用的科学性、合理性提供理论支持。

Abstract

Objective:

To determine the content of index components in different parts of

Gardenia jasminoides

(pericarp

seeds

whiskers)

study the fingerprint

and compare the contents and compositions differences of different parts of

jasminoides

in order to provide the theoretical basis for different efficacies of

jasminoides

pericarp and seeds

explore the exploitation and utilization values of

jasminoides

whiskers

and avoid waste of gardenia medicinal resources.

Method:

The contents of geniposide and crocetin Ⅰ was were determined by HPLC

the content of total iridoid glycosides was determined by ultraviolet spectrophotometry

and three index components in different parts of

jasminoides

were analyzed. HPLC fingerprints of different parts of

jasminoides

were collected

the common pattern of HPLC fingerprints of different parts of

jasminoides

of different origins and with different processing methods was established

and the similarity evaluation software was used for data analysis; comparative analysis on fingerprints of different parts of

jasminoides

was conducted.

Result:

Content change of index components in

jasminoides

pericarp and seeds from Henan

Fujian and Jiangxi were the same. Content of geniposide: Fujian

Henan

Jiangxi

the contents of three components in

jasminoides

pericarp from Fujian were much higher than those from Henan and Jiangxi

the contents of crocetin Ⅰ and total iridoid glycosides: Fujian

Jiangxi

Henan

the contents of total iridoid glycosides from Fujian

Jiangxi were much higher than those from Henan. The order of three index components in

jasminoides

whiskers from different origins from high to low

the content of geniposide and crocetin Ⅰ was Fujian

Jiangxi and Henan

the content of total iridoid glycosides was Fujian

Jiangxi

Henan.In the same part

there were 22 common peaks in the fingerprints of

jasminoides

pericarp

except for S13-S15

the similarity of other samples were more than 0.9; the fingerprints of

jasminoides

seeds had 22 common peaks

except for S22-S30

the similarities of other samples were more than 0.9; the fingerprints of

jasminoides

whiskers had 16 common peaks

except for S7-S9

the similarities of other samples were more than 0.9.In different parts

the fingerprints of

jasminoides

whiskers were significant different from those of pericarp and seeds

the number of peaks in

jasminoides

whiskers reduced

the order of height of peaks 2

5 of

jasminoides

from high to low were whiskers

gardenia

seeds. There was not peak X in the seeds

the height of peak X of gardenia in whiskers was higher than that in pericarp

except for the peak 17

the height of all peaks in seeds were higher than that in whiskers.

Conclusion:

There are significant differences in the contents of index components in

jasminoides

pericarp and seeds. The content of total glycosides in gardenia is high

suggesting that it can be used to extract total iridoid glycosides. The fingerprints can reflect the content difference and species distribution of different parts of

jasminoides

so as to provide theoretical support for the studies for pharmacodynamic material basis of

jasminoides

and the scientificity and rationality of the separate application of

jasminoides

pericarp and seeds.

关键词

栀子皮栀子仁栀子须含量测定指纹图谱

Keywords

pericarp of Gardenia jasminoidesseeds of Gardenia jasminoideswhiskers of Gardenia jasminoidescontent determinationfingerprint

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