Objective:

2

To determine the content of index components in different parts of

Gardenia jasminoides

(pericarp

seeds

whiskers)

study the fingerprint

and compare the contents and compositions differences of different parts of

G

.

jasminoides

in order to provide the theoretical basis for different efficacies of

G

.

jasminoides

pericarp and seeds

explore the exploitation and utilization values of

G

.

jasminoides

whiskers

and avoid waste of gardenia medicinal resources.

Method:

2

The contents of geniposide and crocetin Ⅰ was were determined by HPLC

the content of total iridoid glycosides was determined by ultraviolet spectrophotometry

and three index components in different parts of

G

.

jasminoides

were analyzed. HPLC fingerprints of different parts of

G

.

jasminoides

were collected

the common pattern of HPLC fingerprints of different parts of

G

.

jasminoides

of different origins and with different processing methods was established

and the similarity evaluation software was used for data analysis; comparative analysis on fingerprints of different parts of

G

.

jasminoides

was conducted.

Result:

2

Content change of index components in

G

.

jasminoides

pericarp and seeds from Henan

Fujian and Jiangxi were the same. Content of geniposide: Fujian

>

Henan

>

Jiangxi

the contents of three components in

G

.

jasminoides

pericarp from Fujian were much higher than those from Henan and Jiangxi

the contents of crocetin Ⅰ and total iridoid glycosides: Fujian

>

Jiangxi

>

Henan

the contents of total iridoid glycosides from Fujian

Jiangxi were much higher than those from Henan. The order of three index components in

G

.

jasminoides

whiskers from different origins from high to low

the content of geniposide and crocetin Ⅰ was Fujian

>

Jiangxi and Henan

the content of total iridoid glycosides was Fujian

>

Jiangxi

>

Henan.In the same part

there were 22 common peaks in the fingerprints of

G

.

jasminoides

pericarp

except for S13-S15

the similarity of other samples were more than 0.9; the fingerprints of

G

.

jasminoides

seeds had 22 common peaks

except for S22-S30

the similarities of other samples were more than 0.9; the fingerprints of

G

.

jasminoides

whiskers had 16 common peaks

except for S7-S9

the similarities of other samples were more than 0.9.In different parts

the fingerprints of

G

.

jasminoides

whiskers were significant different from those of pericarp and seeds

the number of peaks in

G

.

jasminoides

whiskers reduced

the order of height of peaks 2

3

5 of

G

.

jasminoides

from high to low were whiskers

>

gardenia

>

seeds. There was not peak X in the seeds

the height of peak X of gardenia in whiskers was higher than that in pericarp

except for the peak 17

the height of all peaks in seeds were higher than that in whiskers.

Conclusion:

2

There are significant differences in the contents of index components in

G

.

jasminoides

pericarp and seeds. The content of total glycosides in gardenia is high

suggesting that it can be used to extract total iridoid glycosides. The fingerprints can reflect the content difference and species distribution of different parts of

G

.

jasminoides

so as to provide theoretical support for the studies for pharmacodynamic material basis of

G

.

jasminoides

and the scientificity and rationality of the separate application of

G

.

jasminoides

pericarp and seeds.