Comparison of Volatile Oil of Domestic and Foreign Panacix Quinquefolii Radix by HS-SPME/GC-MS

Yu SI; Yun-he LIU; Zhong-yao WANG; Jin-ping LIU; Ping-ya LI; Yu-feng JIAO

doi:10.13422/j.cnki.syfjx.20191815

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Comparison of Volatile Oil of Domestic and Foreign Panacix Quinquefolii Radix by HS-SPME/GC-MS

Resource and Quality Evaluation | 更新时间：2021-02-09

- Comparison of Volatile Oil of Domestic and Foreign Panacix Quinquefolii Radix by HS-SPME/GC-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 18, Pages: 130-138(2019)
- 作者机构：
  
  吉林大学　药学院　天然药物研究中心，长春　 130021
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191815
  CLC： R284.1; R282.6; R22; R243
- Published：20 September 2019，
  
  Published Online：10 July 2019，
  
  Received：23 April 2019，
- 稿件说明：
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Yu SI, Yun-he LIU, Zhong-yao WANG, et al. Comparison of Volatile Oil of Domestic and Foreign Panacix Quinquefolii Radix by HS-SPME/GC-MS. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(18):130-138(2019)
DOI：

Yu SI, Yun-he LIU, Zhong-yao WANG, et al. Comparison of Volatile Oil of Domestic and Foreign Panacix Quinquefolii Radix by HS-SPME/GC-MS. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(18):130-138(2019) DOI： 10.13422/j.cnki.syfjx.20191815.

摘要

目的：

对不同产地与不同年限的西洋参的挥发性化学成分进行测定分析，为西洋参的进一步开发和利用提供理论依据。

方法：

采用顶空固相微萃取(HS-SPME)技术结合气相色谱-质谱联用法(GC-MS)对西洋参的挥发性成分进行提取、分析与鉴定，采用色谱峰面积归一化法确定各成分的相对含量。

结果：

西洋参样品中共鉴定出151种成分，包括烃类99种、醇酚类21种、醛类7种，酮类8种，酯类1种，杂环类及其他成分15种。3年生吉林白山产西洋参根中鉴定出68种成分，质量分数98.27%；4年生吉林白山产西洋参根中鉴定出67种成分，质量分数98.79%；3年生美国产西洋参根中鉴定出65种成分，质量分数95.81%；4年生美国产西洋参根中鉴定出63种成分，质量分数99.67%。4个西洋参样品的挥发油成分中的特有成分分别为24，23，19和23种，共有成分为16种。

结论：

不同产地的不同年限的西洋参的挥发性化学成分的含量和组成存在很大差异，该实验结果为未来西洋参药材质量评价研究以及资源的充分合理开发利用提供借鉴。

Abstract

Objective:

To determine the volatile chemical constituents of Panacix Quinquefolii Radix of different origins and different growth years

in order to provide the theoretical basis for the further development and utilization of Panacix Quinquefolii Radix .

Method:

Headspace solid phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) was used to extract

analyze and identify the volatile constituents of Panacix Quinquefolii Radix . The chromatographic peak area normalization method was used to determine the relative amount of ingredients.

Result:

A total of 151 compounds were identified in Panacix Quinquefolii Radix samples

including 99 hydrocarbons

21 alcohol phenols

7 aldehydes

8 ketones

1 ester

15 heterocyclics and other compounds. Totally 68 kinds of compounds were identified in the roots of three-year-old Jilin Baishan

and the mass fraction accounted for 98.27%

67 compounds were identified in the roots of four-year-old Jilin Baishan

and the mass fraction accounted for 98.79%

65 compounds were identified in 3-year-old Panacix Quinquefolii Radix root

and the mass fraction accounted for 95.81%

and 63 compounds were identified in 4-year-old Panacix Quinquefolii Radix root

and the mass fraction accounted for 99.67%. The specific components of the volatile oil components of the four Panacix Quinquefolii Radix samples were 24

and 23

respectively

and the total composition was 16 species.

Conclusion:

The content and composition of volatile chemical constituents of Panacix Quinquefolii Radix of different origins are very different. This experiment provides a reference for the future quality evaluation of Panacix Quinquefolii Radix medicinal materials

rational development and utilization of resources.

关键词

西洋参挥发性成分气相色谱-质谱联用顶空-固相微萃取

Keywords

Panacix Quinquefolii Radixvolatile componentsgas chromatography-mass spectrometryheadspace solid phase microextraction

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