Study on Mechanism of Dahuang Huanglian Xiexintang in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology

Lan-er SHI; Ke-chao NIE; Wen-jing ZHANG; Mei-si ZHENG; Zhi-qin LIN; Zhang-zhi ZHU

doi:10.13422/j.cnki.syfjx.20191840

您当前的位置：

首页 >

文章列表页 >

Study on Mechanism of Dahuang Huanglian Xiexintang in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology

Data Mining | 更新时间：2021-02-09

- Study on Mechanism of Dahuang Huanglian Xiexintang in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 18, Pages: 160-166(2019)
- 作者机构：
  
  1.广州中医药大学　第一临床医学院，广州　 510405；
  2.广州中医药大学　第一附属医院，广州　 510405
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191840
  CLC： R2-0; R285.5; R289
- Published：20 September 2019，
  
  Published Online：04 June 2019，
  
  Received：04 April 2019，
- 稿件说明：
扫描看全文
Lan-er SHI, Ke-chao NIE, Wen-jing ZHANG, et al. Study on Mechanism of Dahuang Huanglian Xiexintang in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(18):160-166(2019)
DOI：

Lan-er SHI, Ke-chao NIE, Wen-jing ZHANG, et al. Study on Mechanism of Dahuang Huanglian Xiexintang in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(18):160-166(2019) DOI： 10.13422/j.cnki.syfjx.20191840.

摘要

目的：

基于网络药理学方法探讨大黄黄连泻心汤治疗2型糖尿病的作用机制。

方法：

借助中药系统药理学技术平台(TCMSP)获取大黄黄连泻心汤主要化学成分、对应靶点及靶标基因，借助人类基因数据库(GeneCards)获取2型糖尿病相关靶标基因，将药物靶标基因与疾病靶标基因映射得到大黄黄连泻心汤作用于2型糖尿病的预测靶点。利用Cytoscape3.7.1软件构建中药化合物-靶点网络及蛋白与蛋白相互作用网络(PPI)，利用DAVID 6.8在线工具进行潜在基因的基因本体(GO)分析和京都基因及基因组百科全书(KEGG)通路富集分析。

结果：

大黄黄连泻心汤作用于2型糖尿病的有效成分有17个，相关靶点94个，关键有效成分17个，关键靶点16个。GO分析结果显示，大黄黄连泻心汤治疗2型糖尿病潜在基因的生物功能主要涉及氧化应激、细胞凋亡、蛋白结合、炎症反应等；KEGG通路富集结果显示，大黄黄连泻心汤治疗2型糖尿病潜在基因的通路主要涉及低氧诱导因子(HIF)，肿瘤坏死因子(TNF)，磷脂酰肌醇-3激酶/蛋白激酶B(PI3K/Akt)，核转录因子-

B(NF-кB)，血管内皮生长因子(VEGF)等信号通路。

结论：

大黄黄连泻心汤治疗2型糖尿病是多成分、多靶点、多通路的复杂过程，主要通过参与氧化应激、细胞凋亡、蛋白结合、炎症反应等发挥治疗2型糖尿病的作用。

Abstract

Objective:

To explore the mechanism of Dahuang Huanglian Xiexintang in the treatment of type 2 diabetes mellitus based on network pharmacology.

Method:

Major chemical constituents

corresponding targets and target genes of Dahuang Huanglian Xiexintang were obtained by Traditional Chinese Medicine Systems Pharmacology(TCMSP)

and target genes of type 2 diabetes mellitus were obtained by GeneCards. The target genes of drug and disease were mapped to predict target genes of Dahuang Huanglian Xiexintang for type 2 diabetes mellitus. Cytoscape3.7.1 software was used to construct the compound-target network and protein-protein interaction network (PPI) of traditional Chinese medicine. Gene ontology (GO) analysis of potential genes and enrichment analysis of gene encyclopedia kyoto encyclopedia of genes and genomes (KEGG) pathway were carried out using DAVID 6.8 online tool.

Result:

There were 17 active ingredients

94 related targets

17 key active ingredients and 16 key targets in Dahuang Huanglian Xiexintang on type 2 diabetes mellitus. GO analysis showed that the biological functions of potential genes of Dahuang Huanglian Xiexintang in the treatment of type 2 diabetes were mainly related to oxidative stress

apoptosis

protein binding

inflammatory reaction

et al. KEGG pathway enrichment results showed that the pathways of potential genes of Dahuang Huanglian Xiexintang in the treatment of type 2 diabetes mainly involved hypoxia inducible factor(HIF)

tumor necrosis factor(TNF)

phosphatidylinositol 3 kinase/protein kinase B(PI3K/Akt)

nuclear transcription factor-кB(NF-кB)

and vascular endothelial growth factor(VEGF) signaling pathways.

Conclusion:

Dahuang Huanglian Xiexintang is a complex process of multi-component

multi-target and multi-pathway in the treatment of type 2 diabetes mellitus. It plays an important role in the treatment of type 2 diabetes mellitus by participating in oxidative stress

apoptosis

protein binding and inflammatory reaction.

关键词

大黄黄连泻心汤2型糖尿病网络药理学作用机制

Keywords

Dahuang Huanglian Xiexintangtype 2 diabetes mellitusnetwork pharmacologymechanism of action

references

GE Q, CHEN L, TANG M, et al. Analysis of mulberry leaf components in the treatment of diabetes using network pharmacology [J].Eur J Pharmacol, 2018, 833: 50-62.

Shaw J E, Sicree R A, Zimmet P Z. Global estimates of the prevalence of diabetes for 2010 and 2030 [J].Diabetes Res Clin Pract, 2010, 87(1): 4-14.

GE Q, CHEN L, CHEN K P. Treatment of diabetes mellitus using iPS cells and spice polyphenols [J].J Diabetes Res, 2017, 2017: 5837804.

Marijana T, Cesare C. Type 2 diabetes mellitus and atrial fibrillation: from mechanisms to clinical practice [J].Arch Cardiovasc Dis, 2015, 108(4): 269-276.

王艳梅，王根杰，张树林，等．临床常用降糖药物的不良反应及防治策略[J]．中国医院药学杂志，2015，35(24)：2233-2236．

张媛媛，张进军，刘怀珍，等．二黄参苓合剂联合治疗早期糖尿病肾病气阴两虚夹痰瘀证的疗效与机制探讨[J]．江西中医药大学学报，2018，30(2)：40-43．

吴青华，李冰涛，涂珺．复方中药治疗糖尿病的研究进展[J]．中国中药杂志，2019，44(6)：1104-1109．

武海英．从火热病机论大黄黄连泻心汤在糖尿病中的应用[J]．中医学报，2016，31(10)：1491-1499．

周强．基于真实世界和临床研究的清热降浊法治疗2型糖尿病人群疗效分析[D]．北京：中国中医科学院，2014．

李泮霖，苏薇薇．网络药理学在中药研究中的最新应用进展[J]．中草药，2016，47(16)：2938-2942．

汝锦龙．中药系统药理学数据库和分析平台的构建和应用[D]．杨凌：西北农林科技大学，2015．

RU J, LI P, WANG J, et al. TCMSP: a database of systems pharmacology for drug discovery from herbal medicines [J].J Cheminform, 2014, 6(1): 13.

YANG Y F, YAN L, WANG J H, et al. Systematic investigation of Ginkgo biloba leaves for treating cardio-cerebrovascular diseases in an animal model [J].ACS Chem Biol, 2017, 12(5): 1363-1372.

卢文丹，李莉，申艳佳，等．基于血管舒缩相关GPCR靶点的小续命汤网络药理学研究[J]．中国中药杂志，2018，43(23)：4698-4708．

Von M C, Jensen L J, Snel B, et al. STRING: known and predicted protein-protein associations, integrated and transferred across organisms [J].Nucleic Acids Res, 2005, 33(1): 433-437.

Botusan I R, Sunkari V G, Savu O, et al. Stabilization of HIF-1ais critical to improve wound healing in diabetic mice [J].PNAS, 2009, 105: 19426-19431.

Changtao J, Aijuan Q, Tsutomu M, et al. Disruption of hypoxia-inducible factor 1 in adipocytes improves insulin sensitivity and decreases adiposity in high-fat diet-fed mice [J].Diabetes, 2011, 60(10): 2484-2495.

张秋菊，李能莲，马亚伟，等．大黄素对低氧环境下SGC7901细胞HIF-1α和E-cadherin的影响[J]．中医研究，2018，31(10)：52-55．

Kramer C K, Zinman B, Retnakaran R. Short-term intensive insulin therapy in type 2 diabetes mellitus: a systematic review and Meta-analysis [J].Lancet Diabetes Endocrinol, 2013, 1(1): 28-34.

印永．2型糖尿病运用早期胰岛素强化治疗的效果观察[J]．中国社区医师，2016，32(30)：59-60．

王军，鲁盈，杨汝春，等．大黄素防治糖尿病大鼠早期肾损伤的实验研究[J]．浙江医学，2000，22(12)：723-726．

张蕙彦．慢性乙型肝炎病人2型糖尿病流行情况调查与相关因素分析[J]．中国卫生产业，2014，11(33)：56-57．

Lijnen H R, Christiaens V, Scroyen L, et al. Impaired adipose tissue development in mice with inactivation of placental growth factor function [J].Diabetes, 2006, 55(10): 2698-2704.

夏启松，刘静维，孙仁宇，等．大黄素对人肺腺癌A549细胞体外增殖凋亡及VEGF和TNF-α分泌的影响[J]．肿瘤防治研究，2010，37(4)：387-391．

HUANG X J, LIU G H, GUO J, et al. The PI3K/Akt pathway in obesity and type 2 diabetes [J].Int J Biol Sci, 2018, 14(11): 1483-1496.

宋冰，刘学政．大黄素对2型糖尿病小鼠血糖、胰岛素水平的影响及机制探讨[J]．山东医药，2011，51(38)：32-33．

ZHANG X Q, XU C F, YU C H, et al. Role of endoplasmic reticulum stress in the pathogenesis of nonalcoholic fatty liver disease [J].World J Gastroenterol, 2014, 30(7): 1768-1776.

陈素领，周杰超，张杰，等．大黄素通过抑制FOXO1活性减轻NO对神经细胞的损伤[J]．生物化学与生物物理进展，2016，43(11)：1076-1085．

LI J, Siegel M, YUAN M, et al. Estrogen enhances neurogenesis and behavioral recovery after stroke [J].J Cereb Blood Flow Metab, 2011, 31(2): 413-425.

Mcconathy J, Sheline Y I. Imaging biomarkers associated with cognitive decline: a review [J].Biol Psychiatry, 2015, 77(8): 685-692.

Le M C, CHU K, HU M, et al. Estrogens protect pancreatic beta-cells from apoptosis and prevent insulin-deficient diabetes mellitus in mice [J].P Nat Acad Sci USA, 2006, 103 (24): 9232-9237.

Tiano J P, Delghingaro-Augusto V, Le M C, et al. Estrogen receptor activation reduces lipid synthesis in pancreatic islets and prevents β cell failure in rodent models of type 2 diabetes [J].J Clin Invest, 2011, 121 (8): 3331-3342.

吕游．炎症因子诱导胰岛β细胞凋亡模型中CK2/NF-κB信号通路的作用研究[D]．长春：吉林大学白求恩第一医院，2018．

ZHU L, HAN J, YUAN R, et al. Berberine ameliorates diabetic nephropathy by inhibiting TLR4/NF-κB pathway [J].Biol Res, 2018, 51(1): 9.

刁红杰，鲁燕．Toll样受体4介导的炎症反应与肥胖和胰岛素抵抗的研究进展[J]．中国糖尿病杂志，2016(11)：1044-1048．

Kim J J, Sears D D. TLR4 and insulin resistance [J].Gastroenterol Res Pract, 2010, 2010(10): 212563.

Jeong J J, Jang S E, Hyam S R, et al. The rhizome mixture of anemarrhena asphodeloides and coptidis chinensis ameliorates acute and chronic colitis mice by inhibiting the binding of lipopolysaccharide to TLR4 and IRAK1 phosphorylation [J].Evid Based Complement Alternat Med, 2014, 10: 1-11.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Pharmacological Mechanism of Xiao Xianxiongtang in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology

Efficacy Evaluation of Ethyl Acetate Fraction of Ipomoea muricatum in Prevention and Treatment of Alcoholic Gastric Ulcer

Mechanism of Dried Fruiting Bodies of Fomes officinalis Against Alzheimer's Disease： Based on Network Pharmacology and In Vitro Experimental Verification

Chemical Components and Mechanism of Qiling Wenshen Formula in Treating Polycystic Ovary Syndrome

Mechanism of Cervi Cornu Pantotrichum in Treatment of Osteoarthritis Based on Network Pharmacology

Related Author

Ke-chao NIE

Wen-jing ZHANG

Mei-si ZHENG

Chuang LIU

XIANG Xi

GUO Hui

GONG Man

ZHANG Yang

Related Institution

School of Pharmacy， Academy of Chinese Medical Sciences， Henan University of Chinese Medicine

College of Pharmacy of Xinjiang Medical University

School of Pharmacy，China Pharmaceutical University

Development Center of Modern Chinese Medicine，Research Institute of Tasly Holding Group Co. Ltd.

State Key Laboratory of Critical Technology in Innovative Chinese Medicine， Tasly Pharmaceutical Group Co. Ltd.

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰