葛根散对酒精性ED大鼠阴茎平滑肌组织结构NOS活力及<italic style="font-style: italic">α</italic>-SMA，Cx43，TGF-<italic style="font-style: italic">β</italic><sub>1</sub> mRNA和蛋白表达的影响

王庆学; 张琪其; 刘春艳; 罗仕艳; 柴艺汇

doi:10.13422/j.cnki.syfjx.20192105

您当前的位置：

首页 >

文章列表页 >

葛根散对酒精性ED大鼠阴茎平滑肌组织结构NOS活力及α-SMA，Cx43，TGF-β₁ mRNA和蛋白表达的影响

药理 | 更新时间：2021-02-09

- 葛根散对酒精性ED大鼠阴茎平滑肌组织结构NOS活力及α-SMA，Cx43，TGF-β₁ mRNA和蛋白表达的影响
- Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β₁ mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats
- 中国实验方剂学杂志 2019年25卷第24期页码：43-47
- 作者机构：
  
  贵州中医药大学，贵阳　 550025
- 作者简介：
  
  王庆学，硕士，从事中医基础理论研究，E-mail：957784686@qq.com
  柴艺汇，硕士，从事中西医结合基础研究， E-mail：846956178@qq.com
- 基金信息：
  
  贵州省中医药管理局项目(QZYY-2014-024);国家级大学生创新创业训练计划项目(贵中医大创合字（2018）2号)
- DOI：10.13422/j.cnki.syfjx.20192105
  中图分类号： R2-0; R22; R285.5; R289
- 纸质出版日期：2019-12-20，
  
  网络出版日期：2019-07-18，
  
  收稿日期：2019-04-01，
- 稿件说明：
扫描看全文
王庆学, 张琪其, 刘春艳, 等. 葛根散对酒精性ED大鼠阴茎平滑肌组织结构NOS活力及α-SMA，Cx43，TGF-β₁ mRNA和蛋白表达的影响[J]. 中国实验方剂学杂志, 2019,25(24):43-47.

Qing-xue WANG, Qi-qi ZHANG, Chun-yan LIU, et al. Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β₁ mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019,25(24):43-47.
王庆学, 张琪其, 刘春艳, 等. 葛根散对酒精性ED大鼠阴茎平滑肌组织结构NOS活力及α-SMA，Cx43，TGF-β₁ mRNA和蛋白表达的影响[J]. 中国实验方剂学杂志, 2019,25(24):43-47. DOI： 10.13422/j.cnki.syfjx.20192105.

Qing-xue WANG, Qi-qi ZHANG, Chun-yan LIU, et al. Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β₁ mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019,25(24):43-47. DOI： 10.13422/j.cnki.syfjx.20192105.

摘要

目的：

探讨中药解酒方葛根散对酒精性勃起功能障碍(ED)大鼠阴茎平滑肌组织结构一氧化氮合酶(NOS)活力及

-平滑肌肌动蛋白(

-SMA)，缝隙连接蛋白基因43(Cx43)，转化生长因子-

(TGF-

) mRNA和蛋白表达的影响，为临床应用葛根散治疗酒精性ED提供实验依据。

方法：

将SD大鼠随机分为5组，分别为正常组、模型组、葛根散低、中、高剂量组(5，10，20 g·kg

－1

)；除正常组外，其他各组按照15 mL·kg

－1

·d

－1

给予乙醇干预30 min后给药。比色法检测酒精性ED大鼠阴茎平滑肌组织NOS活力；实时荧光定量聚合酶链式反应法(Real-time PCR)，蛋白免疫印迹法(Western blot)检测酒精性ED大鼠阴茎平滑肌组织

-SMA，Cx43，TGF-

mRNA及蛋白表达的情况。

结果：

与正常组比较，模型组大鼠阴茎平滑肌组织NOS活力，

-SMA，Cx43 mRNA及蛋白表达量显著下降(

0.05)，TGF-

mRNA及蛋白表达量显著上升(

0.05)。与模型组比较，葛根散低、中、高剂量组均可显著上调酒精性ED大鼠阴茎组织结构NOS活力及Cx43 mRNA及蛋白表达量(

0.05)，同时可显著下调TGF-

mRNA及蛋白表达量(

0.05)，葛根散中、高剂量组均可显著上调酒精性ED大鼠阴茎组织

-SMA mRNA及蛋白表达量(

0.05)。

结论：

葛根散对酒精性ED大鼠阴茎平滑肌组织结构有明显的保护作用，其具体机制可能与调节NOS活力及

-SMA，Cx43，TGF-

mRNA及蛋白表达量有关。

Abstract

Objective:

To investigate the activity of nitric oxide synthase (NOS) and

-smooth actin (

-SMA) in rat penile smooth muscle tissue of rats with alcoholic erectile dysfunction (ED). The effects of protein gene 43 (connexin43

Cx43) and transforming growth factor

(TGF-

) mRNA and protein expressions provide an experimental basis for the clinical application of Gegensan in the treatment of alcoholic ED.

Method:

SD rats were randomly divided into five groups: normal group

model group

and low

medium

high-dose Gegensan groups (5

20 g·kg

-1

). Except the normal group

the other groups were administered with drugs after alcohol intervention for 30 min at 15 mL· kg

-1

·d

-1

. Colorimetric assay was used to detect NOS activity in the penile smooth muscle tissue of alcoholic ED rats. Quantitative real-time fluorescence polymerase chain reaction(Real-time PCR) and Western blot were used to detect

-SMA

Cx43

TGF-

mRNA and protein expressions in smooth muscle tissue of alcoholic ED rats.

Result:

Compared with the normal group

the expressions of NOS

-SMA and Cx43 mRNA and protein in the penile smooth muscle of the model group decreased significantly (

0.05)

while the expressions of TGF-

mRNA and protein increased significantly (

0.05). Compared with the model group

low/medium/high-dose Gegensan groups could significantly up-regulate the NOS activity and Cx43 mRNA and protein expressions in the penile tissue of alcoholic ED rats (P

0.05)

while significantly down-regulate TGF-

mRNA expression

and

-SMA mRNA and protein expressions in the penis tissue of rats with alcoholic ED were significantly up-regulated (

0.05).

Conclusion:

Gegensan has an obvious protective effect on the structure of penile smooth muscle of alcoholic ED rats. The specific mechanism may be related to the regulation of NOS activity and a-SMA

Cx43 and TGF-

mRNA and protein expressions.

关键词

葛根散乙醇勃起功能障碍一氧化氮合酶α-平滑肌肌动蛋白缝隙连接蛋白基因43转化生长因子-β1

Keywords

Gegensanalcoholerectile dysfunctionnitric oxide synthaseα-smooth actingap junction protein gene 43transforming growth factor-β1

references

Barr T, Helms C, Grant K, et al. Opposing effects of alcohol on the immune system [J].Prog Neuropsychopharmacol Biol Psychiatry, 2015, 65: 242-251.

Szabo G, Saha B. Alcohol' s effect on host defense [J].Alcohol Res, 2015, 37(2): 159-170.

Halmenschlager G, Rhoden E L, Motta G A, et al. Testosterone replacement maintains smooth muscle content in the corpuscavernosum of orchiectomized rats [J].Asian J Urol, 2017, 4(4): 223-229.

唐东昕，杨柱，龙奉玺．葛根散对结直肠癌小鼠模型肝脏微环境ICAM-1表达的影响[J]．时珍国医国药，2012，23(9)：2213-2214．

王亚南，刘儒林，程秀臻，等．酒精中毒大鼠实验模型的研究[J]．中国康复理论与实践，2006，12(4)：319-320．

ZHANG J, WU X J, ZHUO D X, et al. Effect of tankyrase 1 on autophagy in the corpus cavernosum smooth muscle cells from ageing rats with erectile dysfunction and its potential mechanism [J].Asian J Androl, 2010, 12(5): 744-52.

陈逢志．血小板源性生长因子BB下调myocardin在低氧诱导的大鼠阴茎海绵体平滑肌细胞表型转化中的作用[D]．广州：南方医科大学，2015．

Goldstein I, Jones L A, Belkoff L H, et al. Avanafil for the treatment of erectile dysfunction: a multicenter, randomized, double-blind study in men with diabetes mellitus [J].Mayo Clin Proc, 2012, 87(9): 843-52.

闫翠，周政，梁碧荣，等．养心康片调节自噬对慢性心力衰竭小鼠心肌纤维化的影响[J]．中国实验方剂学杂志，2019，25(3)：53-58．

陈婉媚，杨其运，卞军，等．尿源干细胞治疗海绵体神经损伤性勃起功能障碍大鼠模型的实验研究[J]．中华男科学杂志，2018，24(6)：483-490．

张培培，陈红波，鲁科达，等．加味黄风汤对 IgA 肾病大鼠肾组织α-SMA及TGF-β表达的影响[J]．中华中医药学刊，2017，35(3)：673-676．

Edwards J P, Hand T W, Morais da Fonseca D, et al. The GARP/Latent TGF-βl complex on Treg cells modulates the induction of peripherally derived Treg cells during oral tolerance [J].Eur J Immunol, 2016, 46(6): 1480-1489.

黄小珊，张世田，唐汉庆，等．壮通饮预处理对冠心病血瘀证大鼠AngⅡ，AT-1R，Cx43及其对再灌注损伤的影响[J]．中国实验方剂学杂志，2018，24(16)：157-162．

王庆学，柴艺汇，田兴中，等．葛根散对酒伤雄性大鼠睾丸组织病理损伤的影响[J]．时珍国医国药，2019，30(1)：74-76．

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

益肺化痰汤减轻哮喘脾虚证大鼠气道高黏状态的作用与机制

加味右归丸联合督脉灸治疗肾阳亏虚型勃起功能障碍的疗效评价及对性激素水平、阴茎血流动力学的影响

人参二醇组皂苷对心肌梗死犬血清一氧化氮、一氧化氮合酶水平的影响

双根清脑颗粒对血管性痴呆模型大鼠行为学及血清超氧化物岐化酶、丙二醛、一氧化氮、一氧化氮合酶的影响

葛根散对酒精性ED大鼠阴茎平滑肌组织结构NOS活力及α-SMA，Cx43，TGF-β1 mRNA和蛋白表达的影响

Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β1 mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats

DOI：10.13422/j.cnki.syfjx.20192105

摘要

Abstract

关键词

Keywords

references

葛根散对酒精性ED大鼠阴茎平滑肌组织结构NOS活力及α-SMA，Cx43，TGF-β₁ mRNA和蛋白表达的影响

Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β₁ mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats