To discuss status and proposals of applying proteomics to research essence of liver injury.Looking through the related literatures from home and abroad

then analyzing and compiling them.①Proteomics technique has been applied to many types of liver injury induced by drug

carbon tetrachloride（CCl4）

alcohol

lipopolysaccharides（LPS）

immune related

thioacetamide

radiation

bromobenzene

hydrazine

or non-alcoholic fatty liver disease（NAFLD）.②The protein samples for analysis are mainly taken from the animal liver

serum

urine

bile acids

mitochondria

etc.③Main analyzing tools of proteomics are two-dimensional gel electrophoresis（2-DE）

Matrix-assisted laser desorption/ionization time of flight mass spectrometry（MALDI-TOF-MS） and multi-stage MS.④Research on proteome mechanism of liver injury needs to be strengthened.⑤Hepatoprotective effect of drugs is not enough through proteomics.We should strengthen researches on essence of liver injury and essence-based hepatoprotection of drugs through proteomics.