Objective:To verify anti-experimental autoimmune neuritis（EAN） activity of dregs in Yisui Tongjing prescription after being extracted by ethanol and optimize its extraction process.Method:EAN rats model was established

then these rats divided into model group

immunoglobulin group

administration group

these rats were administered by the corresponding method

pathomorphological ultrastructure of rats peripheral nerve was observed by electron microscope.With comprehensive score of dry extract quality and yield of protein as index

orthogonal test was adopted to optimize extraction process of water-soluble constituents in Yisui Tongjing prescription with the amount of water

extraction time and times as factors.Result:The situation of demyelination of the administration group was significantly improved

the structure of myelinated fibers myelin lamellar was neat

at the same time you could still see the change in aggregation of collagen fibers and the newborn myelinated nerve fibers.The best extraction process for water-soluble constituents in Yisui Tongjing prescription was:extracted 3 times with 8 times the amount of water for 3 h each time.Conclusion:Water-soluble constituents in Yisui Tongjing prescription had treatment effect for EAN;This optimized extraction technology was simple

stable and suitable for industrial production.