Objective:To establish quantitative and qualitative determination for the quality control standard of K-CoxB-JN.Method: TLC was used to identify Panacis Quiaquefolii Radix

Ophiopogonis Radix

Raphani Semen and Vaccariae Semen.The content of ginsenoside Rg1

Re

Rb1 in K-CoxB-JN was determined by HPLC-ELSD.An Agilent ZORBAX XDB C18（4.6 mm×250 mm

5 μm） was used

and the mobile phase consisted of acetonitrile-0.2% formic acid（gradient elution）.The temperature was set at room condition.The parameter of evaporative light-scattering detector: the temperature of drift tube was at 50 ℃ and the nebulizer nitrogen flow rate was 2.0 L · min-1;gain was 1.Result: The TLC spots were highly clear without the interference of negative sample

and had good separation effect.The linear relationship was obtained within the range of 0.75-3.75

4.5-30 and 6-40 μg for ginsenoside Rg1

Re

Rb1 respectively（r>0.999）.The average recoveries of the three components were 97.10%（RSD 3.96%）

95.15%（RSD 2.21%）

95.60%（RSD 3.00%） respectively.Conclusion: The method appeared to be simple

accurate

sensitive and reliable

and can be used in qualitative and quantitative analysis of K-CoxB-JN.