Objective To establish a sensitive and specific HPLC method for quality control of Ilex hainanensis Merr.．Methods HPLC method was applied for quality assessment of Ilex hainanensis Merr.． HPLC analysis was performed on a HanbonC18 column (250mm×4．6mm，5 m)．The mobile phase consisted of acetonitrile (solvent B) and watercontaining0.1% (v/v) phosphoric acid (solvent A) at a constant flow rate of 1.0ml/min．An increasing linear gradient (v/v) of solvent B was used. The injection volume was 10μl. The column temperature was set at 25℃. The chromatogram s were monitored at 330nm. Results In different varieties of processing methods of Ilex hainanensis Merr. showing 9 characteristic peaks. It was established from its prepared samples of Ilex hainanensis Merr.．Conclusion The characteristic chromatogram of Ilex hainanensis Merr. with high specificity can be used to control its quality as well as the consistency of processing products.