Objective:To establish the HepG2 cells model of insulin resistance by the method of insulin-induced in vitro

and provide a reliable model of insulin resistance in vitro for the study of the insulin resistance and the type 2 diabetes. Method: RPMI 1640 medium with different insulin concentrations was used to the HepG2 cells

the medium glucose content was detected at different time points by the method of glucose-hexokinase and the HepG2 cell viability of different insulin concentrations was detected by the method of MTT

and to determine the optimal concentration and time of the insulin resistance; then the changes of cell morphology was observed by the oil red O staining; the expression of insulin receptor substance(IRS)-2 mRNA was detected by the method of real-time PCR and the expression of G-6-Pase was detected by the method of ELISA. Result: The best time of insulin resistance was 36 h

the best concentration of insulin resistance was 1×10-8 mol·L-1. After the establishment of the model

the lipid droplets of model cells was significantly increased compared with normal cells; the IRS-2 mRNA expression was significantly reduced compared with normal cells (P＜0.05); the G-6-Pase expression was significantly increased compared with normal cells (P＜0.05). Conclusion: By the method of insulin-induced in vitro

a stable HepG2 insulin resistance model can be established when the 1×10-8mol·L-1 concentration of insulin act on the HepG2 cell for 36 h.