YANG Fei, XU Yan-hao. Rapid Genome DNA Methylation Changes in after Exposure to Elevated Temperature[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 113-116.
YANG Fei, XU Yan-hao. Rapid Genome DNA Methylation Changes in after Exposure to Elevated Temperature[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 113-116.DOI:
Objective: To investigate the effect of elevated temperature in genome cytosine methylation changes in Isatis indigotica. Method: Methylation sensitive amplification polymorphism (MSAP) approach and 36 pairs of selective primers combinations were used in this study. Result: A total of 1 612 bands were obtained.In all
82(5.1%) CCGG sites displayed cytosine methylation changes after exposure to elevated temperature.Among these sites
58(3.6%) CCGG sites had undergone hypermethylation changes and 24(1.5%) CCGG sites undergone demethylation changes. Conclusion: This result provided evidence for genome-wide DNA methylation remodeling occurring immediately after exposure to elevated temperature in I.indigotica.