AB-8型大孔吸附树脂纯化复方通脉颗粒有效部位的工艺优选

陈超; 陈国芹; 陈阿丽; 崔红花; 郭嘉雯; 王淑美

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AB-8型大孔吸附树脂纯化复方通脉颗粒有效部位的工艺优选

更新时间：2024-09-23

- AB-8型大孔吸附树脂纯化复方通脉颗粒有效部位的工艺优选
- Optimization of Purification Technology for Effective Parts from Compound Tongmai Granules by AB-8 Macroporous Absorption Resin
- 中国实验方剂学杂志 2013年19卷第5期页码：4-6
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81073024)
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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陈超, 陈国芹, 陈阿丽, 等. AB-8型大孔吸附树脂纯化复方通脉颗粒有效部位的工艺优选[J]. 中国实验方剂学杂志, 2013,19(5):4-6.

CHEN Chao, CHEN Guo-qin, CHEN A-li, et al. Optimization of Purification Technology for Effective Parts from Compound Tongmai Granules by AB-8 Macroporous Absorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 4-6.
陈超, 陈国芹, 陈阿丽, 等. AB-8型大孔吸附树脂纯化复方通脉颗粒有效部位的工艺优选[J]. 中国实验方剂学杂志, 2013,19(5):4-6. DOI：

CHEN Chao, CHEN Guo-qin, CHEN A-li, et al. Optimization of Purification Technology for Effective Parts from Compound Tongmai Granules by AB-8 Macroporous Absorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 4-6. DOI：

摘要

目的: 优选复方通脉颗粒的大孔树脂纯化工艺。方法: 以总酚酸

葛根素的吸附-洗脱率为指标

采用单因素试验考察上样液质量浓度、径高比、洗脱剂等对复方通脉颗粒大孔树脂纯化工艺的影响。结果: 优选的纯化工艺为上样液质量浓度110 g·L-1

树脂柱径高比1∶10

树脂体积与上样量比2.8∶1(吸附3 h)

加2BV水洗除杂

用6 BV 50%乙醇洗脱

收集洗脱液。结论: 该纯化工艺合理、稳定

可推广于大生产应用。

Abstract

Objective:To optimize purification technology of compound Tongmai granules by macroporous resin. Method: Taking adsorption-desorption rate of total phenolic acid and puerarin as indexes

effect of the concentration of sample solution

diameter-height ratio

elution agent on purification technology of compound Tongmai granules was investigated by single factor test. Result: AB-8 macroporous absorption resin was the best resin

optimum purification technology was as following:the concentration of sample solution 110 g·L-1

diameter-height ratio of resin column 1∶10

ratio of resin-material drug 2.8∶1(adsorbed 3 h)

eluted with 2 BV water

then eluted with 6 BV 50% ethanol

collected eluent. Conclusion: This optimized purification technology was reasonable and stable

it could be extended to large-scale production applications.

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