Objective: To optimize polyamide purification technology of total flavonoids from Mentha haplocalyx. Method: With the content and transfer rate of total flavonoids as indexes

adsorption capacity of 5 different kinds of purification materials for total flavonoids was investigated by single factor test

in order to determine optimum purification material

and adsorption

impurity removing and eluting conditions in purification technology were optimized. Result: Optimum purification conditions were as follows:The concentration of sample solution 0.1 g·mL-1

diameter-height ratio of column 1:9

adsorption rate 2 BV·h-1

proportion between the total amount of solution and resin column 1:6

3 BV of 10% ethanol to wipe off impurity with flow rate of 4 BV·h-1

then 3 BV of 90% ethanol to elute with 8 BV·h-1.After purified by polyamide(30-60 mesh)

purity of total flavonoids was up to 50%. Conclusion: This optimized purification technology was simple and feasible with good reproducibility

it could be used for the separation and the enrichment of total flavonoids from M.haplocalyx.