Objective:To study the molecular mechanism of the treatment in which Xiaoyong Kuidekang would be applied to a model of gastric ulceration in rats. Method: Rats were divided into 5 groups randomly:normal group

sham group

ulcer model group

Xiaoyong Kuidekang group and Omeprazole group.Eight rats in each group.Acetic acid was applied to a certain part of the gastric mucosa to establish the ulcer model.The Xiaoyong Kuidekang treatment group had been administrated with drugs 4 g·kg-1and the Omeprazole group had been administrated with drugs 1.25 g·kg-1 since the second day after the operation.after 10 days The stomach and the serum was taken.The healing process of gastric ulcers was observed and the areas was measured.The content of EGF

VEGF

Bcl-2

and C-myc was measured by ELISA method.Total RNAs were extracted from the ulcer part of the gastric tissue by Trizol method

then the RNAs were transcribed reversely into cDNAs.The relative expression levels of EGF mRNA was measured by the QPCR assay. Result: There was no difference between the normal group and the sham operation group on levels of EGF

VEGF

C-myc and Bcl-2.the concentration of EGF

Bcl-2 in model group were lower than those in the normal group(P<0.05)and The concentration of VEGF

C-myc in model group were higher than those in the normal(P<0.05).The concentration of EGF

VEGF or Bcl-2 in the Xiaoyong Kuidekang group and in the Omeprazole group were much higher than those in the ulcer model group(P<0.05).The concentrations of C-myc in the Xiaoyong Kuidekang group and in the Omeprazole group were much lower than those in the ulcer model group(P<0.05).The mered result of the the rat EGFmRNA expression through the QPCR method is revealed.The expression of EGFmRNA in the Xiaoyong Kuidekang group were much higher than that in the ulcer model group(P<0.05). Conclusion: Xiaoyong Kuidekang can raise the expression of VEGF

EGF in rat serum

promote the growth of gastric epithelial and endothelial cell

promote healing

raise the level of Bcl-2

inhibit apoptosis.At the same time

reduce the level of C-myc

reducing the probability of occurrence of carcinogenesis.