Objective:To establish a UPLC method for simultaneous determination of saikosaponins a

c

d in Bupleurum chinense. Method: Saikosaponins a

c

d in B. chinense were simultaneously determined by UPLC with Waters ACQUITY UPLC BEH C18(2.1 mm ×50 mm

1.7 μm) column. With a gradient program of acetonitrile-water at the flow rate of 0.4 mL·min-1

use an evaporative light-scattering detector (UPLC-ELSD) as the detector. The drift tube temperature was set at 40 ℃; the gas flow rate was 2.5 L·min-1. Result: Saikosaponins a

c

d in B. chinense were separated clearly and respectively in 6 min. The linear ranges of Saikosaponins a

c

d were 0.043 6-0.348 8

0.046 8-0.374 4 and 0.049 2-0.393 6 μg respectively. The RSD of recovery

precision and reproducibility were all less than 3.0%. Conclusion: The method is rapid

simple

reliable and accurate

has been successfully used to the quantification of saikosaponins a

c

d in B. chinense.