LC-MS-MS法测定参体和参须中人参皂苷Rb,Rb和Rc含量

闫寒; 赵松岩; 刘运嘉; 吴睿凡; 范斌; 郭娜; 彭娟

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LC-MS-MS法测定参体和参须中人参皂苷Rb,Rb和Rc含量

更新时间：2024-01-04

- LC-MS-MS法测定参体和参须中人参皂苷Rb,Rb和Rc含量
- Simultaneous Quantitation of Rb, Rb and Rc in Ginseng Tail and the Main Root of Ginseng by LC-MS-MS
- 中国实验方剂学杂志 2013年19卷第6期页码：105-107
- 作者机构：
- 作者简介：
- 基金信息：
  
  中国中医科学院医学实验中心自主课题项目(zz2007002)
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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闫寒, 赵松岩, 刘运嘉, 等. LC-MS-MS法测定参体和参须中人参皂苷Rb,Rb和Rc含量[J]. 中国实验方剂学杂志, 2013,19(6):105-107.

YAN Han, ZHAO Song-yan, LIU Yun-jia, et al. Simultaneous Quantitation of Rb, Rb and Rc in Ginseng Tail and the Main Root of Ginseng by LC-MS-MS[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 105-107.
闫寒, 赵松岩, 刘运嘉, 等. LC-MS-MS法测定参体和参须中人参皂苷Rb,Rb和Rc含量[J]. 中国实验方剂学杂志, 2013,19(6):105-107. DOI：

YAN Han, ZHAO Song-yan, LIU Yun-jia, et al. Simultaneous Quantitation of Rb, Rb and Rc in Ginseng Tail and the Main Root of Ginseng by LC-MS-MS[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 105-107. DOI：

摘要

目的: 建立检测参体和参须中人参皂苷Rb1

Rb2和Rc的LC-MS-MS方法。方法: 应用液质联用

C18色谱柱系统

采用梯度洗脱方法

A-水(0.05%FA)

B-乙腈

A-B=65:35

梯度洗脱(0～3 min

65% B;3～4 min

100% B)

流速0.35 mL·min-1

柱温40 ℃

建立了参体和参须中人参皂苷Rb1

Rb2和Rc含量测定方法并进行了方法学考察。结果: 建立了同时检测参体和参须中人参皂苷Rb1

Rb2和Rc含量测定方法。结论: 该方法准确、简便、快速

可用于参体和参须的质量控制。

Abstract

Objective: To develop a method to determine the content-of ginsengnoside Rb1

Rb2 and Rc in ginseng tail and the main root of ginseng by using HPLC-MS-MS. Method: C18 HPLC column was used in the system including HPLC-MS-MS with a gradient elution program.The mobile phase was consisted of: A for water(0.05%FA)

B for acetonitrile

A: B=65: 35; gradient elution program was as follows:0-3 min

65%(B); 3-4 min

100%(B).The flow rate was 0.35 mL·min-1; column temperature was kept at 40 ℃. Result: Simultaneous quantitation of ginsengnoside Rb1

Rb2 and Rc in ginseng tail and the main root of ginseng by HPLC-MS-MS was developed. Conclusion: The method is accurate

convenient and rapid

which can be applied for the quality control of ginseng tail and the main root of ginseng.

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