Objective: To investigate the effects of Shenlian (SL) extracts on mast cells activation. Method: Rat peritoneal mast cells (RPMC) were collected and seeded on 96-well plates. Compound 48/80 was added into the cultural supernatants after the cells were pretreated with SL extracts for 2 h. Then we collected the supernatants and detected Histamine

5-hydroxytryptamine and tryptase content with ELISA kits. Result: The results show Compound 48/80 increased histamine release from 32.36% to 61.71% (P<0.01). SL extracts(100

50

25

12.5 mg·L-1) reduced RPMC histamine release to 16.24%

26.25%

27.81%

31.50%(P<0.01). Compound 48/80 increased tryptase release from 13.19% to 21.72% (P<0.01)

SL extracts(100

50

25

12.5 mg·L-1) reduced RPMC tryptase release to 8.60%

15.78%

8.80%

12.15% (100

25

12.5 mg·L-1

P<0.01

50 mg·L-1

P<0.05). Compound 48/80 had no obvious effect on RPMC 5-HT release. But 100

50

25 mg·L-1 of SL extracts can increase 5-HT release (100

50 mg·L-1

P<0.01; 25 mg·L-1

P<0.05). Conclusion: We concluded SL extracts can stabilize mast cells.