桑枝中桑皮苷A的含量测定

方婧; 高霏; 吴宏伟; 李韶菁; 许海玉; 杨洪军; 杨岚; 付梅红; 刘峰

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桑枝中桑皮苷A的含量测定

更新时间：2024-09-23

- 桑枝中桑皮苷A的含量测定
- Determination of Mulberroside A in by HPLC
- 中国实验方剂学杂志 2013年19卷第5期页码：80-82
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家"重大新药创制"科技重大专项(2011ZX09201-201-26)
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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方婧, 高霏, 吴宏伟, 等. 桑枝中桑皮苷A的含量测定[J]. 中国实验方剂学杂志, 2013,19(5):80-82.

FANG Jing, GAO Fei, WU Hong-wei, et al. Determination of Mulberroside A in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 80-82.
方婧, 高霏, 吴宏伟, 等. 桑枝中桑皮苷A的含量测定[J]. 中国实验方剂学杂志, 2013,19(5):80-82. DOI：

FANG Jing, GAO Fei, WU Hong-wei, et al. Determination of Mulberroside A in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 80-82. DOI：

摘要

目的: 建立桑枝中桑皮苷A的含量测定方法。方法: Dikma Diamonsil C18色谱柱(4.6 mm×250 mm

5 μm)

流动相为甲醇-水(15∶85)

检测波长324 nm

柱温30 ℃

流速1 mL·min-1。结果: 桑皮苷A在10.34~206.8 μg呈良好线性关系(r=0.999 9)

平均回收率99.8%;11批桑枝药材中桑皮苷A含量在0.10%~1.69%。结论: 此法准确、简便、快速

可用于检测桑枝中桑皮苷A的含量。

Abstract

Objective:To establish a method for the determination of mulberroside A in Ramulus Mori by HPLC. Method: Dikma Diamonsil C18 column (4.6 mm×250 mm

5 μm) was used. The mobile phase consisted of methanol and water (15∶85). The temperature of column was maintained at 30 ℃. The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 324 nm. Result: The calibration curve was in good linearity within the range from 10.34 to 206.8μg (r=0.999 9). The average recovery was 99.8%. The content of mulberroside A in Ramulus Mori in defferent areas was 0.10%-1.69%. Conclusion: The method is simple

rapid as well as precise and reliable

and can be used for the determination of mulberroside A in Ramulus Mori.

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