Objective:To explore the effect of phytohormone on the breaking seed dormancy and plant morphogenesis of Paris polyphylla var.yunnanensis under aseptic culture condition. Method: The mature seeds of P.polyphylla var.yunnanensis were used for the experimental materials. The effects of gibberellic acid (GA3)

trans-zeatin-riboside (ZR)

3-indolyl-acetic acid (IAA)

and active carbon (AC) on the breaking seed dormancy of P.polyphylla var.yunnanensis were investigated under aseptic culture condition by orthogonal experimental design

at the same time

during the aseptic germination process the plant morphogenesis was observed. Result: The best ratio for the seed protrusion of P.polyphylla var.yunnanensis was GA3-ZR-IAA-AC 1.0 mg·L-1∶1.0 mg·L-1∶0.1 mg·L-1∶0.5 g·L-1

and seed protrusion rate is up to 80.00%. The role sequence of four factors on the seed protrusion rate of P.polyphylla var.yunnanensis has been found to decrease in the order: IAA>GA3>ZR>AC. The best ratio for the seed germination of P.polyphylla var.yunnanensis is: GA3-ZR-IAA-AC 1.0 mg·L-1∶1.0 mg·L-1∶0.1 mg·L-1∶0.5 g·L-1

and seed germination rate is up to 66.66%. The role sequence of four factors on the seed germination rate of P.polyphylla var.yunnanensis was found to decrease in the order: AC>IAA>ZR>GA3. Conclusion: Adding the phytohormone GA

ZR

IAA

and AC into the culture medium has very important influence on the breaking seed dormancy

seed protrusion and germination of P.polyphylla var.yunnanensis.