卫气营血辨证肺损伤肺表面活性蛋白mRNA表达的动态变化

邓时贵; 叶莹仪; 彭珍香

您当前的位置：

首页 >

文章列表页 >

卫气营血辨证肺损伤肺表面活性蛋白mRNA表达的动态变化

更新时间：2024-01-04

- 卫气营血辨证肺损伤肺表面活性蛋白mRNA表达的动态变化
- Dynamic Changes of SP mRNAs in Lung Tissues of Rabbits Models with Differentiated Syndromes of Weifen, Qifen, Yingfen, and Xuefen
- 中国实验方剂学杂志 2013年19卷第6期页码：279-282
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省科技厅项目(2009B060300025);广东省中医药管理局项目(2009189)
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
移动端阅览
邓时贵, 叶莹仪, 彭珍香. 卫气营血辨证肺损伤肺表面活性蛋白mRNA表达的动态变化[J]. 中国实验方剂学杂志, 2013,19(6):279-282.

DENG Shi-gui, YE Yin-yi, PENG Zhen-xiang. Dynamic Changes of SP mRNAs in Lung Tissues of Rabbits Models with Differentiated Syndromes of Weifen, Qifen, Yingfen, and Xuefen[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 279-282.
邓时贵, 叶莹仪, 彭珍香. 卫气营血辨证肺损伤肺表面活性蛋白mRNA表达的动态变化[J]. 中国实验方剂学杂志, 2013,19(6):279-282. DOI：

DENG Shi-gui, YE Yin-yi, PENG Zhen-xiang. Dynamic Changes of SP mRNAs in Lung Tissues of Rabbits Models with Differentiated Syndromes of Weifen, Qifen, Yingfen, and Xuefen[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 279-282. DOI：

摘要

目的: 探讨卫气营血辨证肺损伤时肺表面活性蛋白SP-A

SP-B

SP-C mRNA表达随时间变化的规律。方法: 30只新西兰兔随机分为正常对照组和卫气营血辨证肺损伤组(2 h代表卫分组

6 h代表气分组

12 h代表营分组

24 h代表血分组

各1组)

每组6只采用耳缘静脉推注脂多糖(LPS)建立卫气营血辨证肺损伤模型

注射LPS后处死各组动物

采用RT-PCR法检测肺组织SP-A

SP-B

SP-C mRNA表达变化。正常对照组麻醉后取肺组织标本待测。结果: 与正常对照组相比

卫气营血辨证肺损伤卫分、气分、营分、血分各亚组SP-A

SP-B

SP-C mRNA表达显著下降(P<0.05)。SP-C气分组与卫分组相比mRNA表达无显著性差异

其余卫气营血辨证肺损伤各亚组间SP-A

SP-B

SP-C mRNA表达均具有显著性差异(P<0.05)

并随注射LPS时间延长

SP mRNA表达明显降低

其表达与肺损伤程度呈负相关。结论: 卫气营血辨证肺损伤兔肺表面活性蛋白SP-A

SP-B

SP-C mRNA表达呈时间依赖性降低

是卫气营血辨证肺损伤的重要物质基础之一。

Abstract

Objective: To study the dynamic changes of SP mRNAs in lung tissue of animal models with differentiated syndromes of Weifen

Qifen

Yingfen

and Xuefen. Method: Thirty rabbits were randomly divided into normal control group and Weifen

Qifen

Yingfen

and Xuefen groups(2

24 h four subgroups

corresponding to Weifen

Qifen

Yingfen and Xuefen

n=6 each)

Lipopolysaccharides(LPS

1 mg·kg-1)was given by intravenous injection through auricular vein in the groups.Rabbits in each subgroup were killed after injection

lung tissue specimens were collected and tested.Expressions of SP-A

SP-B

SP-C mRNA at different points of time were measured by using real-time PCR.The control group was received no treatment

lung tissue specimen was tested as in the treatment group. Result: Compared with the control group

levels of transcription of SP-A

SP-B

SP-C mRNA were decreased significantly in Weifen

Qifen

Yingfen and Xuefen groups(P<0.05).Levels of transcription of SP-C mRNA showed no significant changes in Weifen and Qifen groups

besides

levels of transcription of SP-A

SP-B

SP-C mRNA among every two subgroups in Weifen

Qifen

Yingfen and Xuefen groups were decreased significantly(P<0.05).In addition

the expression of SP mRNAs was decreased significantly as time passed after the injection of LPS

and the expression was negatively correlated with the degree of lung injury. Conclusion: The changes of the SP-A

SP-B

SP-C mRNA in rabbits following acute lung injury are decreased in a time-dependent manner

which maybe one of the important material basis of acute lung injury induced by syndromes of Weifen

Qifen

Yingfen

and Xuefen.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

清瘟败毒饮及其活性成分防治肺损伤的研究进展

参莲方对颗粒物暴露致大鼠急性肺损伤的保护作用

基于¹H-NMR代谢组学的豨莶草水洗脱部位致大鼠肺损伤作用机制分析

清胰颗粒对重症急性胰腺炎患者并发肺损伤的应用研究

水通道蛋白 mRNA表达与肺卫失宣大鼠肺损伤的相关性及大黄的调节作用