TANG Yun, LIU Han-qing, TANG Hai-tao, et al. Study on HPLC Fingerprint of Pushen Capsule[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 133-137.
TANG Yun, LIU Han-qing, TANG Hai-tao, et al. Study on HPLC Fingerprint of Pushen Capsule[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 133-137.DOI:
Objective: To establish the HPLC fingerprint of Pushen capsule and give a new method for quality control. Method: RP-HPLC was used on a Agilent ZORBAX SB-C18 (4.6 mm×250 mm
5 μm) with the gradient elution solvent system composed of acetonitrile-0.1% H3PO4 water solution; the detection wavelength was 254 nm; sample injection was 10 μL. Result: HPLC fingerprint of Pushen capsule was established. Taking 2
3
5
4'-tetrahydroxystibene-2-O-β-D-glucoside as the reference peak
eighteen common peaks were selected as the fingerprint peaks of Pushen capsule. The similarity between the fingerprint of twelve batches of Pushen capsule samples and reference fingerprint was determined. Conclusion: The established HPLC fingerprint has desirable precision
reproducibility and stability
and can be applied to the quality control of Pushen capsule.