Objective: To find a quality control method of mangiferin

anemarrhena saponin A3

caffeoyl glyceride and ophiopogonin D

which are flavones effective fraction

steroidal saponins effective fraction from Zhimu (Anemarrhenae Rhizoma)

the total phenylic effective fraction and steroidal saponins effective fraction from Baihe (Lilium lancifolium) in Baihe Zhimu decoction respectively. Method: The procedure of HPLC-DAD was performed on the chromatographic column of Yi li-te Hypersil ODS (4.6 mm×250 mm

5 μm) at 25℃. The mobile phase was acetonitrile-0.1% phosphoric acid solution in gradient elution. The flow velocity was 1.0 mL·min-1 and the detection wavelength was set at 258 nm for mangiferin

191 nm for anemarrhena saponin A3

310 nm for caffeoyl glyceride and 200 nm for ophiopogonin D'. Result: Mangiferin showed a good linear relationship in a range from 0.228-1.14 g·L-1; caffeoyl glyceride from 0.072-0.576 g·L-1

anemarrhena saponin A3 from 0.069 25-0.554 g·L-1

ophiopogonin D' from 0.101-0.505 g·L-1. The average recovery was 98.97%

99.32%

97.65% and 96.67% respectively. The limit of quantitation and detection about mangiferin was 0.912 mg·L-1 and 0.456 mg·L-1. The LOQ and LOD about caffeoyl glyceride was 1.44

0.36 mg·L-1. The LOQ and LOD about anemarrhena saponin A3 was 5.54

1.85 mg·L-1. The LOQ and LOD about ophiopogonin D' was 3.16

1.06 mg·L-1. Conclusion: The method was simple and reliable

which can be used for the quantitative determination of mangiferin

caffeoyl glyceride; anemarrhena saponin A3 and ophiopogonin D' as well as quantity control of Baihe Zhimu decoction.