Objective: To establish a HPLC method for determination of puerarin

daidzein-8-C-apiosyl-(1→6) glucoside and daidzin in Radix Pueraria thomsonii. Method: The mobile phrase was methanol-0.02% phosphoric acid (25: 75) with a Diamonsil C18 column (4.6 mm×250 mm

5 μm)

detection wavelength at 250 nm. Result: The accuracy

stability

and reproducibility of this method all met the requirements.The linearity range of puerarin

daidzein-8-C-apiosyl-(1→6) glucoside and daidzin is 0.123-0.737

0.024 9-0.150 μg and 0.021 7-0.130 μg.The average recovery of three components is 99.4%

99.0% and 99.1%.The method was applied to determine the content of four batches of Radix Pueraria thomsonii. Conclusion: The method is rapid

simple and accurate

which can be used in the quality control in Radix Pueraria thomsonii.