构树叶总黄酮对人肝癌细胞HepG-2增殖和凋亡的作用及其机制研究

朱开梅; 姚丽新; 刘建楠; 桂劲松; 顾生玖

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构树叶总黄酮对人肝癌细胞HepG-2增殖和凋亡的作用及其机制研究

更新时间：2024-01-04

- 构树叶总黄酮对人肝癌细胞HepG-2增殖和凋亡的作用及其机制研究
- Effects of Total Flavonoids of on Proliferation and Apoptosis of Hepatocellular Carcinoma HepG-2 Cells
- 中国实验方剂学杂志 2013年19卷第6期页码：270-273
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西科技特派员专项(桂科攻09321056,桂科能129825-21);广西教育厅项目(201202ZD065);桂林市科技攻关项目(20110106-5,20120105-5,20120105-16,20120105-8)
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- 纸质出版日期：2013
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朱开梅, 姚丽新, 刘建楠, 等. 构树叶总黄酮对人肝癌细胞HepG-2增殖和凋亡的作用及其机制研究[J]. 中国实验方剂学杂志, 2013,19(6):270-273.

ZHU Kai-mei, YAO Li-xin, LIU Jian-nan, et al. Effects of Total Flavonoids of on Proliferation and Apoptosis of Hepatocellular Carcinoma HepG-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 270-273.
朱开梅, 姚丽新, 刘建楠, 等. 构树叶总黄酮对人肝癌细胞HepG-2增殖和凋亡的作用及其机制研究[J]. 中国实验方剂学杂志, 2013,19(6):270-273. DOI：

ZHU Kai-mei, YAO Li-xin, LIU Jian-nan, et al. Effects of Total Flavonoids of on Proliferation and Apoptosis of Hepatocellular Carcinoma HepG-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 270-273. DOI：

摘要

目的: 探讨构树叶总黄酮(TFBP)对人肝癌细胞(HepG-2)增殖和凋亡的作用。方法: 采用细胞增殖实验(MTT法)

取对数生长期HepG-2

随机分为药物组和对照组

用3

12 g·L-1不同质量浓度构树叶总黄酮作用HepG-2

分别干预24

72 h。通过流式细胞仪检测在以上质量浓度下作用细胞48 h后的细胞凋亡率

Western blont法检测以上质量浓度构树叶总黄酮作用细胞48 h后Bcl-2基因、促凋亡基因Bax表达的情况。结果: 各质量浓度构树叶总黄酮对HepG-2的生长有显著的抑制作用

呈明显的时间和剂量依赖性

经不同质量浓度的构树叶总黄酮作用48 h后HepG-2凋亡率显著增加

药物组与对照组比较为(0.478±0.085 vs 0.498±0.014; 0.354±0.004 vs 0.498±0.014;0.218±0.075 vs 0.498±0.014)有显著性差异

P<0.05

抑癌基因Bcl-2表达量呈逐渐下降的趋势

促凋亡基因Bax表达量随着质量浓度的升高表达量呈现上升趋势。结论: 构树叶总黄酮可以有效的抑制HepG-2的生长

诱导细胞凋亡

其诱导该细胞凋亡的作用可能是通过下调抑癌基因Bcl-2的表达

可为肝癌的生物学治疗提供新的方法。

Abstract

Objective: To investigate the effects of total flavonoids of Broussonetia papyrifera on growth and apoptosis of hepatocellular carcinoma HepG-2 cells. Method: The MTT assay was used

and the hepatocellular carcinoma HepG-2 cells in logarithmic phase of growth were randomLy divided into drug group and control group

with the concentration of 3

12 g·L-1 total flavonoids of B. papyrifera in hepatocellular carcinoma HepG-2 cells

intervention durations were 24

72 h accordingly.The percentage of apoptotic cell were detected by FCM.The expression of protein Bcl-2 was analyzed by Western blot. Result: Inhibition rate of the hepatocellular carcinoma HepG-2 was obviously enhanced along with increase of the total flavonoids of B. papyrifera concentration

the effect is also showed in time course.Flow cytometry study revealed

with the concentration increased

the percentage of apoptotic cells raised after 48h.The expression of protein Bcl-2 was down-regulated along with increase of the total flavonoids of B. papyrifera concentration. Conclusion: The quartering can effectively inhibit tumor cell growth and induced tumor cells into apoptosis.It may be associate with the low expression of protein Bcl-2 in hepatocellular carcinoma cell line HepG-2.

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